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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

27卷

期数:

2021年03期

页码:

252-255

栏目:

临床医学

出版日期:

2021-05-30

文章信息/Info

Title:

Disrupted gut microbiota affects the formation of carotid atherosclerosis by promoting inflammatory responses

文章编号:

1006-8147(2021)03-0252-04

作者:

韩晋¹; 2; 陈淑媛²; 毓青¹

1.天津医科大学总医院神经内科，天津 300052；2.天津港口医院神经内科，天津 300456

Author(s):

HAN Jin¹; 2; CHEN Shu-yuan²; YU Qing¹

1.Department of Neurology, General Hospital, Tianjin Medical University, Tianjin 300052，China；2.Department of Neurology, Port Hospital of Tianjin, Tianjin 300456，China

关键词:

肠道菌群失调; 颈动脉粥样硬化; 炎性反应

Keywords:

disrupted gut microbiota; carotid atherosclerosis; inflammatory response

分类号:

R543.5

DOI:

-

文献标志码:

A

摘要:

目的：观察肠道菌群失调对颈动脉的影响，探讨肠道菌群失调对颈动脉粥样硬化形成的机制。方法： C57BL/6小鼠随机分为对照组（n=15）和肠道菌群失调组（n=15）。肠道菌群失调组小鼠予头孢曲松钠（0.1 g/mL，2 mL）灌胃，对照组小鼠予同体积生理盐水灌胃，2次/d，共持续6周。灌胃结束后禁食12 h，行肠道细菌培养及计数，观察颈动脉苏木素伊红（HE）染色图片，测颈动脉内膜横截面积和狭窄率，采用逆转录-聚合酶链反应（RT-PCR）检测颈动脉白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）和转化生长因子-β1（TGF-β1）mRNA的表达，使用Western印迹法观察颈动脉核因子κB p65（NF-κB p65）的表达情况。结果： 两组在肠道菌群组成（肠球菌、乳酸杆菌及双歧杆菌）上存在差异（t=2.72、2.27、2.56， 均P<0.05）。与对照组比较，肠道菌群失调组小鼠颈动脉内膜增厚[（0.14±0.01）mm2 vs. （0.45±0.08）mm2，P<0.05]，管腔狭窄率升高[（2.15±0.92）% vs.（18.31±6.82）%，P<0.05]，炎症细胞浸润较多，IL-6、TNF-α、TGF-β1 mRNA以及NF-κB p65蛋白含量明显增多，差异均有统计学意义（t=2.15、2.67、3.16、6.95，均P<0.05）。结论： 肠道菌群调节炎症反应，影响颈动脉粥样硬化的形成。

Abstract:

Objective: To observe the effect of disrupted gut microbiota on carotid artery，and explore the mechanism of carotid atherosclerosis caused by disrupted gut microbiota. Methods： C57BL/6 mice were divided into two groups randomly，the control group （n=15） and the disrupted gut microbiota group（n=15）. The disrupted gut microbiota group was given ceftriaxone by gavage（0.1 g/mL，2 mL），and the control group was given the same volume of saline，twice a day for 6 weeks. Fasted for 12 hours at the end of gavage，gut microbiota was cultured and counted，the images of hematoxylin eosin（HE） staining of carotid artery were observed，and the cross sectional area and stenosis rate of carotid arterial intima were measured. The expression of interleukin-6（IL-6），tumor necrosis factor-α（TNF-α） and transforming growth factor-β1（TGF-β1）mRNA in carotid artery was assessed by reverse transcription-polymerase chain reaction（RT-PCR） method. The expression of nuclear factor kappa-B p65（NF-κB p65） was detected by Western blotting method. Results： The composition of intestinal flora （enterococcus，lactic acid bacteria and bifidobacterium） was different between the two groups（t=2.72，2.27，2.56，all P<0.05）. Compare with the control group，carotid intima thickness[（0.14±0.01） mm2 vs.（0.45±0.08） mm2，P<0.05]，lumen stenosis rate[（2.15±0.92）% vs.（18.31±6.82）%，P<0.05]，infiltrated inflammatory cells，and the expression of IL-6,TNF-α，TGF-β1 mRNA and NF-κB p65 protein（t=2.15，2.67，3.16，6.95，all P<0.05） were significantly increased in the disrupted gut microbiota group. The results showed there were significant differences between two groups. Conclusion： Disrupted gut microbiota affectes the formation of carotid atherosclerosis by regulating the inflammatory response.

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相似文献/References:

备注/Memo

备注/Memo:

作者简介 韩晋（1980-），女，主治医师，硕士在读，研究方向：癫痫；
通信作者：毓青，E-mail：1433428114@qq.com。

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更新日期/Last Update: 2021-05-30