|本期目录/Table of Contents|

[1]李 丹.31例多发性硬化患者T淋巴细胞亚群及相关细胞因子 的检测[J].天津医科大学学报,2019,25(01):80-83.
 LI Dan.Detection of Tlymphocyte subsets and related cytokines in 31 cases of multiple sclerosis[J].Journal of Tianjin Medical University,2019,25(01):80-83.
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31例多发性硬化患者T淋巴细胞亚群及相关细胞因子 的检测(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
25卷
期数:
2019年01期
页码:
80-83
栏目:
临床医学
出版日期:
2019-01-20

文章信息/Info

Title:
Detection of Tlymphocyte subsets and related cytokines in 31 cases of multiple sclerosis
文章编号:
1006-8147(2019)01-0080-04
作者:
李 丹
(威海市立医院南院区检验科,威海 264200)
Author(s):
LI Dan
(Department of Laboratory, Southern Hospital, Weihai Municipal Hospital, Weihai 264200, China)
关键词:
多发性硬化T淋巴细胞亚群Th17细胞Treg细胞细胞因子
Keywords:
multiple sclerosis T lymphocyte subsetsT help 17 cell Treg cellscytokines
分类号:
R744.5
DOI:
-
文献标志码:
A
摘要:
目的:探讨多发性硬化(MS)患者静脉血T淋巴细胞亚群及相关细胞因子的意义。方法:采用流式细胞术(FCM)检测31例MS患者治疗前(MS组)、泼尼松治疗4周后(泼尼松组)及20例健康者(正常组)外周血Th1(CD4+IFN-γ+)、Th2(CD4+IL-4+)、Th17(CD4+IL-17+)、Treg(CD4+FoxP3+)、Tc1(CD4-CD8+IFN-γ+)和Tc2(CD4-CD8+IL-4+)T淋巴细胞亚群变化;流式微球芯片捕获技术(CBA)检测血浆细胞因子IFN-γ、TNF-α、IL-17A、IL-4和IL-10的含量。结果:(1)FCM分析结果显示,与正常组比较,MS组Th1细胞和Th17细胞均明显升高(P<0.05);Treg细胞和Tc1细胞显著下降(P<0.01),Th17/Treg比例明显升高(P<0.01);与MS组比较,泼尼松组Tc1、Tc2和Treg细胞百分率均明显升高(P<0.01),而Th1和Th17细胞显著下降(P<0.05),Th17/Treg比值降低(P<0.01);(2)CBA法检测结果显示,与正常组比较,MS组IFN-γ和IL-17A含量升高(P<0.05;P<0.01),IL-4和IL-10含量明显降低(P<0.01);与MS组比较,泼尼松组IL-17A含量明显降低(P<0.01),IL-4和IL-10均显著升高(P<0.01)。结论:MS的发生可能主要与Th17上调和Treg下调有关,提示临床上针对MS靶向下调Th17和上调Treg,纠正Th17/ Treg比例失衡,具有潜在应用价值。
Abstract:
Objective: To investigate the levels of T lymphocyte subsets and related cytokines in 31 cases of the patients with multiple sclerosis(MS)and their significance to clinic. Methods: The lymphocyte subsets including Th1(CD4+IFN-γ+),Th17(CD4+IL-17+),Treg(CD4+FoxP3+),Tc1(CD4-CD8+IFN-γ+) and Tc2(CD4-CD8+IL-4+)in peripheral blood mononuclear cells were determined by flow cytometry in three different groups;concentrations of plasma cytokines including IFN-γ,TNF-α,、IL-17A,IL-4 and IL-10 were determined by cytometric bead array. Results: Compared with normal control group,the percentages of Th1 and Th17 cells wereincreased significantly(P<0.05),while Treg and Tc1 cells were decreased significantly(P<0.01),the proportion of Th17/Treg was increased significantly in MS group (P<0.01); compared with MS group, the percentages of Tc1,Tc2 andTreg cells were increased significantly(P<0.05),while Th17 and Th1 cells were reduced significantly(P<0.01),and the proportion of Th17/Treg was reduced significantly (P<0.01) in Prednisone group; Compared with normal control group ,the concentration of IFN-γ and IL-17A were increased significantly(P<0.05;P<0.01), while the concentration of IL-4 and IL-10 were decreased significantly in MS group(P<0.01); compared with MS group, the concentration of IL-17 A were decreased obviously(P<0.05;P<0.01), while the concentration of IL-4 and IL-10 were increased significantly in prednisone group(P<0.01).Conclusion: The mechanism of multiple sclerosis might relate to Treg down -regulation,and Th17 cells up-regulation. Experiments in this paper show that it has potential application value forTreg up-regulation,and Th17 cells down-regulation,which could be used asan immunomdulatory therapy in the patients with clinical multiple sclerosis.

参考文献/References:


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备注/Memo

备注/Memo:

作者简介 李丹(1986-),女,检验医师,硕士,研究方向:临床化学与免疫学;E-mail:yiran7519@foxmail.com。
更新日期/Last Update: 2019-03-01