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[1]赵莉逦,刘晓丹,PUREVDORJ Narangerel,等.系统性老年性淀粉样变患者血清转甲状腺素蛋白出现三种修饰基团减少[J].天津医科大学学报,2019,25(01):10-13.
　ZHAO Li-li,LIU Xiao-dan,PUREVDORJ Narangerel,et al.Three modified groups to decrease in serum transthyroxine protein in patients with systemic senile amyloidosis[J].Journal of Tianjin Medical University,2019,25(01):10-13.
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系统性老年性淀粉样变患者血清转甲状腺素蛋白出现三种修饰基团减少(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
25卷
期数:
2019年01期
页码:
10-13
栏目:
基础医学
出版日期:
2019-01-20

文章信息/Info

Title:
Three modified groups to decrease in serum transthyroxine protein in patients with systemic senile amyloidosis
文章编号:
1006-8147(2019)01-0010-05
作者:
赵莉逦; 刘晓丹; PUREVDORJ Narangerel; 王 然; 李 蕾; 孙续国
（天津医科大学医学检验学院临床检验和血液教研室，天津 300203）
Author(s):
ZHAO Li-li; LIU Xiao-dan; PUREVDORJ Narangerel; WANG Ran; LI Lei; SUN Xu-guo
（Department of Clinical Laboratory and Blood, School of Medical Laboratory, Tianjin Medical University, Tianjin 300203, China)
关键词:
甲状腺素运载蛋白; 化学修饰; 老年系统性淀粉样变; 飞行时间质谱
Keywords:
transthyretin;  chemical modification;  senile systemic amyloidosis;  SELDI-TOF-MS
分类号:
Q7
DOI:
-
文献标志码:
A
摘要:
目的：探讨转甲状腺素（TTR）蛋白代谢修饰与淀粉样变形成的分子机制。方法:本研究首先利用ProteomeLabPF-2D系统依据蛋白质等电点分选各个pH值范围蛋白质，然后利用抗人TTR抗体，点免疫印迹实现鉴定TTR蛋白存在于被收集的范围。利用硫黄素T（ThT）试剂，测定各个收集部分溶液中淀粉样变的含量，进一步利用飞行质谱的表面增强激光解吸/电离时间（SELDI-TOF-MS）分析各个部分TTR蛋白的修饰类型。结果:系统性老年性淀粉样变（SSA）患者和健康志愿者血清pH范围分别为 8.1～8.0，4.0～4.6及6.8～5.8范围时出现高丰度蛋白。并且SSA患者pH6.8~5.8范围淀粉样变含量明显高于对照组（P＜0.05），其中SSA组与健康组相比，血TTR 蛋白N端氨基酸修饰基团包括磷酰基（Phosphoryl）、羟基（Hydroxyl）、葡萄糖胺（O-GlcNac）基团修饰阴性。结论：SSA患者血TTR 蛋白N端氨基酸修饰基团Phosphoryl、Hydroxyl、O-GlcNac的减少可能与淀粉样蛋白的形成有关。
Abstract:
Objective: To explore TTR event protein metabolism and the molecular mechanism of amyloid formation. Methods: We first used ProteomeLabPF - 2D system to separate protein on the basis of protein isoelectric point(IP) in each pH range and then western blot was applied to identify the TTR range. We used sulfur flavin T (ThT) reagent quantitative to sort out the collection part of the content of amyloidosis, further flight mass spectrometry of surface-enhanced laser desorption/ionization- time (SELDI TOF MS) to analyze each part of TTR event protein modification type. Results: It was found that SSA patients and healthy volunteers whose serum pH were 8.0- 8.6, pH 4.0-4.6 and pH 5.8-6.8 had a high abundance of protein. Moreover, during pH 6.8 to 5.8, the amyloidosis content of SSA patient group had significantly higher pH than the control group. The TTR N-terminal amino acid modified groups: Phosphoryl, Hydroxyl, O - GlcNac were negative. Conclusion: The reduction of TTR event N-terminal amino acid modified protein was found in SSA patients and Phosphoryl (Phosphoryl), Hydroxyl (Hydroxyl), glucosamine (O - GlcNac) may be associated with the formation of amyloid protein.

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备注/Memo

备注/Memo:
基金项目 国家自然科学基金资助项目（30973157）；天津市科学技术基金国家资助（15JCYBJC268000）
作者简介 赵莉逦（1990-），女，初级检验师，硕士，研究方向：智能医学检测；通信作者：孙续国，E-mail:sunxuguo@tijmu.edu.cn。
更新日期/Last Update: 2019-03-01