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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

24卷

期数:

2018年01期

页码:

7-9

栏目:

基础医学

出版日期:

2018-01-20

文章信息/Info

Title:

Construction of ovarian cancer OVCAR3 cell line for the stable expression of SND1

文章编号:

1006-8147(2018)01-0007-03

作者:

杨 扬¹; 2; 白 虹²; 辛灵彪³

(1.天津市血液中心,天津 300110;2.天津医科大学免疫学系,天津 300070； 3.天津医科大学生物化学与分子生物学系,天津 300070)

Author(s):

YANG Yang¹; 2;  BAI Hong²;  XIN Ling-biao³

（1. Tianjin Blood Centre, Tianjin 300110, China;2. Department of Immunology ,Tianjin Medical University, Tianjin 300070, China; 3.Department of Biochemistry and Molecular Biology, Tianjin Medical University, Tianjin 300070, China)

关键词:

SND1' target="_blank" rel="external">">SND1; 慢病毒载体; OVCAR3细胞; 卵巢癌

Keywords:

staphylococcal nuclease domain containing 1' target="_blank" rel="external">">staphylococcal nuclease domain containing 1; lentiviral vector;  OVCAR3 cells;  ovarion cancer

分类号:

R737.31

DOI:

-

文献标志码:

A

摘要:

目的： 通过慢病毒载体构建过表达SND1的OVCAR3稳定的表达株。方法：重组的pLVX-FLAG-SND1表达载体与包装质粒共同转染293T细胞，获得携带FLAG-SND1的重组慢病毒。病毒感染的OVCAR3细胞经Hygromycin B筛选出稳定表达株。用Western blot方法检测SND1蛋白表达。结果：用病毒感染OVCAR3细胞，药物筛选后获得的过表达稳定株中SND1蛋白表达水平明显增高。结论：利用慢病毒表达载体pLVX-FLAG-SND1成功感染并筛选出稳定表达SND1的OVCAR3细胞株，为进一步研究SND1在卵巢癌中的作用提供了体外细胞系模型。

Abstract:

Objective: To screen the human ovarian cancer cell line OVCAR3 which can stably expresses SND1 protein. Methods：The recombinant plasmids pLVX-FLAG-SND1 was co-transfected into 293T cell line with packaging plasmids, and the culture supermatant containing the lentiviral particles was collected to infect OVCAR3 cell. Hygromycin B was used to screen the infected cells, so as to obtain a cell line for stable expression. The expression level of SND1 was detected by Western-blot. Results：OVCAR3 cells were infected by the lentivirus and the expression of SND1 was significantly up-regulated following screening with Hygromycin B. Conclusion：The cell line OVCAR3 stably overexpressing SND1 could be established successfully, thus providing an in vitro model for further study of molecular function and mechanism of SND1 in human ovarion cancer.

参考文献/References:

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备注/Memo

备注/Memo:

基金项目 国家自然科学基金资助项目（31370749、31571380）；天津市应用基础与前沿技术研究计划（青年基金项目）（15JCQNJC09900）

作者简介 杨扬（1982-），女，硕士 ，研究方向：免疫学；通信作者：辛灵彪，E-mail：xinlingbiao@tmu.edu.cn。

常用功能

更新日期/Last Update: 2018-01-19