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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

23卷

期数:

2017年03期

页码:

203-207

栏目:

基础医学

出版日期:

2017-05-18

文章信息/Info

Title:

Effect of morinda officinalis and estrogen on RANK and CAII expression in osteoclasts of osteoporosis rat

文章编号:

1006-8147(2017)03-0203-05

作者:

杨博辰 朱明喜 曹一维

（河南中医药大学第三附属医院骨科， 郑州 450000）

Author(s):

YANG Bo-chen; ZHU Ming-xi; CAO Yi-wei

(Department of Orthopaedics, The Third Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou 450000,China)

关键词:

巴戟天' target="_blank" rel="external">">巴戟天; 雌激素; 骨质疏松; 破骨细胞; 核激活因子kb受体; 碳酸酐酶II; 大鼠

Keywords:

morinda officinalisestradiol; osteoporosis;  osteoclasts; RANK; CAII; rat

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分类号:

R589.5

DOI:

-

文献标志码:

A

摘要:

目的： 探讨巴戟天、雌激素对骨质疏松大鼠破骨细胞核激活因子kb受体（RANK）和碳酸酐酶II（CAII）的表达水平间关系。方法： 选择月龄为4个月的SPF级成年健康雌性SD大鼠，建立去势大鼠骨质疏松模型，分离大鼠原代破骨细胞并分为6组：空白对照组、17b-雌二醇10^-6mmol/L组、0.1 g/d巴戟天组、0.5 g/d巴戟天组、1.0g/d巴戟天组、17b-雌二醇10^-6mmol/L和1.0g/d巴戟天组进行培养，观察比较各组 CAII、 RANK mRNA表达等指标差异。结果： 17b-雌二醇10^-6mmol/L组、0.5 g/d巴戟天组、1.0g/d巴戟天组、17b-雌二醇10^-6mmol/L联合1.0g/d巴戟天组破骨细胞数量均较空白对照组明显减少，而17b-雌二醇10^-6mmol/L联合1.0g/d巴戟天组减少最明显（P<0.05），0.1 g/d巴戟天组与对照组无显著差异（P＞0.05）；17b-雌二醇10^-6mmol/L组、0.5 g/d巴戟天组、1.0g/d巴戟天组、17b-雌二醇10^-6mmol/L联合1.0g/d巴戟天组骨吸收陷窝面积相均低于空白对照组（P<0.05），而17b-雌二醇10^-6mmol/L联合1.0g/d巴戟天组骨吸收陷窝面积明显低于其他组（P<0.05），0.1 g/d巴戟天组与对照组无显著差异（P＞0.05）；17b-雌二醇10^-6mmol/L组、0.5 g/d巴戟天组、1.0g/d巴戟天组、17b-雌二醇10^-6mmol/L联合1.0g/d巴戟天组CAII、RANK mRNA基因表达水平明显低于空白对照组（P<0.05），0.1 g/d巴戟天组与对照组无显著差异（P＞0.05），17b-雌二醇10^-6mmol/L组和0.5 g/d巴戟天组RANK mRNA基因、CAII mRNA基因表达水平差异无统计学意义（P＞0.05），17b-雌二醇10^-6mmol/L联合1.0g/d巴戟天组CAII、RANK mRNA基因表达水平最低（P<0.05）。结论： 巴戟天联合雌激素能有效诱导骨质疏松大鼠破骨细胞内RANK和CAII处于低表达状态，从而有效预防骨质疏松的发生。

Abstract:

Objective ： To investigate the function of morinda officinalis and estrogen on RANK and CAII expression in osteoclasts of osteoporosis rat. Methods： Four-months old SFP healthy female rats were chosen for osteoporosis rat model construction. Primary osteoclast cells were isolated and divided into 6 groups, then they were treated with control, 10^-6mmol/L 17- estradiol, 0.5 g/d morinda officinalis，1.0g/d morinda officinalis, 10^-6mmol/L 17-estradiol combined with1.0g/d morinda officinalis respectively. Then osteoclast number, the area of bone resorption, the mRNA expression of carbonic anhydrase II and beta receptor activator of nuclear factor were calculated. Results： Osteoclast numbers for 10^-6mmol/L 17 - estradiol, 0.5 g/d morinda officinalis,1.0g/d morinda officinalis, and 10^-6mmol/L 17-estradiol combine with 1.0g/d morinda officinalis were less than the control（P<0.05）. The group of 0.1 g/d morinda officinalis had no significance with the control（P＞0.05）. The group of 10^-6mmol/L 17-estradiol combined with 1.0g/d morinda officinalis was the least（P<0.05）. The expressions of carbonic anhydrase II and beta receptor activator of nuclear factor in 10_-6mmol/L 17 - estradiol, 0.5g/d morinda officinalis, 1.0g/dmorinda officinalis,10^-6mmol/L 17-estradiol combine with 1.0g/d morinda officinalis groups lower lower than the control（P<0.05）. The group of 0.1 g/d morinda officinalis had no significance compared with the control（P＞0.05）. The group of 10^-6mmol/L 17β-estradiol combined with 1.0g/d morinda officinalis had the lowest expression（P<0.05）. But the expressions of carbonic anhydrase II and beta receptor activator of nuclear factor between 10^-6mmol/L 17 - estradiol group and 0.5 g/d morinda officinalis group had no significant difference（P＞0.05）. Conclusion：Estradiol combined with morinda officinalis could decrease the expression of RNAK and CAII to prevent osteoporosis in osteoporosis rat.

参考文献/References:

[1]郑素玉，陈健，何剑全，等．巴戟天含药血清对成骨-破骨细胞共育体系CAII、NFAT2mRNA表达的影响[J]．中国骨质疏松杂志，2013，19（2）：120
[2]王建伟，郭杨，周信，等．金匮肾气丸联合葡萄糖酸钙对去势大鼠骨微结构的影响[J]．中国老年学杂志，2012，32（2）：313
[3]刘雷，郭元晖，辛海量，等．仙茅苯甲酸酯类酚苷对去卵巢骨质疏松大鼠的作用[J]．中西医结合学报，2012，10（12）：1419
[4]赵雪芳，李惠玲，赵银卿，等．低剂量雌激素在绝经后骨质疏松症防治中的作用[J]．河北医学，2011，17（6）：735
[5]Fuentes E, Palomo I. Mechanisms of endothelial cell protection by hydroxycinnamic acids[J]. Vascul Pharmacol, 2014, 63(3): 155
[6]王啸，刘禄林，高超，等．雌激素与铁在骨吸收中的拮抗效应及其机制[J]．中华医学杂志，2014，94（33）：2613
[7]王汝杰，刘复州，沈伟伟，等．Jagged1活化Notch通路促进RAW 264.7向破骨分化但抑制增殖[J]．中国免疫学杂志，2014，01（7）：865
[8]Daniel M, Tollefsbol T. Epigenetic linkage of aging, cancer and nutrition[J]. J Exp Biol, 2015, 218(Pt 1): 59
[9]范金柱，杨柳，罗卓荆，等．雌激素对绝经后骨质疏松患者骨髓间充质干细胞Notch信号通路的影响[J]．中华骨质疏松和骨矿盐疾病杂志，2013，6（3）：232
[10]Li Y, Lü S, Tang Gui, et al. Effect of morinda officinalis capsule on osteoporosis in ovariectomized rats[J]. Chin J Nat Med, 2014, 12(3): 204

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备注/Memo

备注/Memo:

作者简介 杨博辰（1983-），男，主治医师，学士，研究方向：骨科; E-mail:460769744@qq.com。

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更新日期/Last Update: 2017-05-18