子查询返回的值不止一个。当子查询跟随在 =、!=、<、<=、>、>= 之后，或子查询用作表达式时，这种情况是不允许的。 B7.1/GM-CSF修饰的PC3-DC融合疫苗在体外抗肿瘤免疫效应-《天津医科大学学报》
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[1].B7.1/GM-CSF修饰的PC3-DC融合疫苗在体外抗肿瘤免疫效应[J].天津医科大学学报,2017,23(03):194-198.
　HAO Xiao-dong,LI Chang-ying,BO Zhi-qiang,et al. Antitumor effect of PC3-DC fusion vaccines modified by B7.1 / GM- CSF in vitro[J].Journal of Tianjin Medical University,2017,23(03):194-198.
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B7.1/GM-CSF修饰的PC3-DC融合疫苗在体外抗肿瘤免疫效应(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
23卷
期数:
2017年03期
页码:
194-198
栏目:
基础医学
出版日期:
2017-05-18

文章信息/Info

Title:
 Antitumor effect of PC3-DC fusion vaccines modified by B7.1 / GM- CSF in vitro
文章编号:
1006-8147(2017)03-0194-05
作者:
-
(1天津医科大学第二医院泌尿外科，天津 300211;2.天津市泌尿外科研究所,天津 300211)
Author(s):
HAO Xiao-dong1; 2; LI Chang-ying2; BO Zhi-qiang1; 2; DING Hao1; 2; ZHANG Chang-wen1; 2; ZHANG Zhi-hong1; 2
(1 . Department of Urology, The Second Hospital, Tianjin Medical University, Tianjin 300211,China; 2: Tianjin Institute of Urology, Tianjin 300211,China )
关键词:
ＤＣ细胞; 前列腺癌细胞; 融合疫苗; Ｂ７.１; ＧＭ－ＣＳＦ; 免疫治疗
Keywords:
dendritic ; cell;  prostate cancer;  fusion vaccine;  B7.1;  GM-CSF;  Immunotherapy
分类号:
R737.25
DOI:
-
文献标志码:
A
摘要:
 目的：制作B7.1/GM-CSF修饰的DC融合疫苗，并观察其在体外的特异性抗肿瘤免疫效应。方法：制备完整表达GM-CSF的PC3细胞，从前列腺癌患者外周血中分离DC，应用电融合法融合DC和GM-CSF-PC3，构建B7.1/GPI蛋白并锚定于融合细胞表面；应用免疫荧光观察融合细胞形态及锚定稳定性；流式细胞进行融合细胞表形测定；ELISA试剂盒检测IFN-γ分泌情况；MTT及LDH试剂盒测定融合细胞刺激同源异体T淋巴细胞增殖和细胞毒性效应。结果：成功制备GM-CSF-PC3细胞及B7.1/GPI蛋白， 成功分离DC并表达DC分子标记；B7.1/GM-CSF融合疫苗高表达DC分子标记及前列腺癌标记；T细胞增值实验证明融合疫苗具有强烈的免疫刺激活性；细胞毒性实验证明了融合细胞具有比对照组更有效的肿瘤杀伤作用。 结论：电融合技术可成功诱导DC与GM-CSF-PC3融合，体外试验中特定修饰的融合疫苗可显著的提升抗肿瘤免疫效应。
Abstract:
 Objective: To construct the fusion vaccine with dendritic cells and prostate cancer cells (PC3) and modify it by B7.1/GM-CSF, and test the anti-tumor immunological reaction of the fusion vaccine in vitro. Methods: Fusion cells was electrofusioned by DC and GM-CSF-PC3, and B7.1/GPI protein was constructed and anchored to the fusion cell surface; Cell morphology and anchoring stability were determined by immunofluorescence, and fusion cell phenotypic were determined by flow cytometry; fusion vaccines stimulated homologous proliferation and cytotoxic effect of allogeneic T lymphocytes were determined by MTT and LDH kit. Results: B7.1/GM-CSF fusion vaccine highly expressed DC molecular markers and prostate cancer markers; B7.1/GM-CSF fusion vaccines strongly promoted the proliferation of T cells and induced a significantly tumor-specific cytotoxic T lymphocyte (CTL) response and killed the PC3 cells effectively in vitro. Conclusion: the DC and GM-CSF-PC3 can be successfully fused by electrical fusion technique, and the specific modified fusion vaccine can significantly enhance the anti-tumor immune effect in vitro.

参考文献/References:

[1] Siegel R L, Miller K D, Jemal A. Cancer statistics, 2016[J]. CA Cancer J Clin, 2016,66(1):7
[2] de Bono J S, Logothetis C J, Molina A, et al. Abiraterone and increased survival in metastatic prostate cancer[J]. N Engl J Med, 2011,364(21):1995
[3] Scher H I, Fizazi K, Saad F, et al. Increased survival with enzalutamide in prostate cancer after chemotherapy[J]. N Engl J Med, 2012,367(13):1187
[4] Buttigliero C, Tucci M, Bertaglia V, et al. Understanding and overcoming the mechanisms of primary and acquired resistance to abiraterone and enzalutamide in castration resistant prostate cancer[J]. Cancer Treat Rev, 2015,41(10):884
[5] Saad F, Miller K. Current and Emerging Immunotherapies for Castration-resistant Prostate Cancer[J]. Urology, 2015,85(5):976
[6] Vuk-Pavlovic S, Bulur P A, Lin Y, et al. Immunosuppressive CD14+HLA-DRlow/- monocytes in prostate cancer[J]. Prostate, 2010,70(4):443
[7] Zheng Q, Long J, Jia B, et al. Transforming growth factor-beta1 deteriorates microrheological characteristics and motility of mature dendritic cells in concentration-dependent fashion[J]. Clin Hemorheol Microcirc, 2014,56(1):25
[8] Schreiber R D, Old L J, Smyth M J. Cancer immunoediting: integrating immunity’s roles in cancer suppression and promotion[J]. Science, 2011,331(6024):1565
[9] Neves A R, Ensina L F, Anselmo L B, et al. Dendritic cells derived from metastatic cancer patients vaccinated with allogeneic dendritic cell-autologous tumor cell hybrids express more CD86 and induce higher levels of interferon-gamma in mixed lymphocyte reactions[J]. Cancer Immunol Immunother, 2005,54(1):61
[10] Kichler-Lakomy C, Budinsky A C, Wolfram R, et al. Deficiences in phenotype expression and function of dentritic cells from patients with early breast cancer[J]. Eur J Med Res, 2006,11(1):7

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备注/Memo

备注/Memo:

基金项目 国家自然科学基金资助项目（81301949）；天津市自然科学基金资助项目（16jcybjc26500）

作者简介 郝晓东( 1991-)， 男 ，硕士在读，研究方向： 泌尿外科疾病 ；

通信作者：张志宏 ，E-mail：zhihongzhang1967@163.com。



 

更新日期/Last Update: 2017-05-18