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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

22卷

期数:

2016年01期

页码:

17-20

栏目:

基础医学

出版日期:

2016-01-20

文章信息/Info

Title:

Effect of laser micropore porcine acellular demal matrix on vascularization of wounds with full-thickness burn

文章编号:

1006-8147（2016）01-0017-04

作者:

盛小辉¹; 朱宝昌¹ ; 冯世海² ; 刘群²; 于维陆³

(1.天津医科大学研究生院,天津 300070; 2.天津市第四医院烧伤科,天津 300222;3.天津市托福医用原子能科技有限公司,天津 300192)

Author(s):

SHENG Xiao-hui¹ ; ZHU Bao-chang¹; FENG Shi-hai²; LIU Qun² ;  Yu Wei-lu³

(1.Graduate School, Tianjin Medical University, Tianjin 300070,China;2.Department of Burn, The Fourth Hospital of Tianjin,Tianjin 300222,China;3.Tianjin Toefl Medical Atomic Energy Technology Co.,Ltd,Tianjin 300192,China)

关键词:

真皮替代物; 脱细胞真皮; 猪; 微孔; 皮肤移植; 烧伤; 大鼠

Keywords:

dermal substitutes;  acellular dermal matrix;  microporous; skin transplantation;  burns; rat

分类号:

R644

DOI:

-

文献标志码:

A

摘要:

目的： 探讨新的脱细胞方法配合激光打孔制备的激光微孔脱细胞猪真皮支架对大鼠Ⅲ度皮肤烧伤创面血管化的影响。方法： 将激光微孔脱细胞猪真皮(A组)、普通打孔脱细胞猪真皮（B组）两种真皮支架分别移植至Ⅲ度皮肤烧伤清创后创面，另设无真皮支架对照创面（C组），1周后加植表皮，大体观察植入真皮支架1、2、3周创面的修复情况，并通过HE染色和免疫组化标记创面中CD31、α-SMA表达阳性血管并计数。结果： 制备的脱细胞猪真皮呈瓷白色，有光泽，柔软；显微镜下未见细胞残留。加植表皮后，A组较B组存活率高。两组支架植入后及无支架对照组1～3周创面中CD31、α-SMA表达阳性血管数持续增加，其中两组真皮支架均较无支架组多，差异有统计学意义(P<0.05)； A组显著高于B、C组(P<0.05)。结论：该方法制备的激光微孔脱细胞猪真皮基质能快速血管化，提高表皮存活率，为一种较理想的真皮替代物。

Abstract:

Objective ： To explore the influence of the new method—laser micropore porcine acellular demal matrix in vascularization of wounds in rats with full-thickness burn. Methods ：Laser micropore porcine acellular demal matrix (A group) and porcine acellular demal matrix (B group) were respectively transplanted on the rats with full-thickness burn after wound debridement, and there were no dermal in the control group(C group). Then skin was transplanted into the rats of the three groups one week later. The reparation of dermal implant wound was recorded after 1,2,and 3 weeks respectively. At the same time, CD31, α-SMA positive signals detected by immunohistochemical staining and HE staining were used to observe the effect of repairation. Results：The well-prepared laser microspore porcine acellular dermal matrix was characterized by its porcelain white color, softness and good elasticity. Histological examination revealed that laser microspore porcine acellular dermal matrix was totally devoid of epidermis and cellular components. Group A has higher survival rate than group B. From 1th to 3th week, the number of vessels with CD31 and α-SMA expression were continuously increased in the three groups. The number of vessels with CD31 and α-SMA expression in group A and B were significantly higher than in group C (P<0.05). The difference was statistically significant (P<0.05). Conclusion Laser microspore porcine acellular demal matrix can be used as an ideal dermal substitute due to its ability of initiating revascularization rapidly that(改为which) may increase the survival rate of the grafted skin.

参考文献/References:

[1]柴家科，杨红明，李利根，等.去细胞异体真皮、去细胞猪真皮和自体刃厚皮移植在临床中的应用[J].中华外科杂志,2000,38(10):790

[2] 吴志谷，耿淼，付小兵，等．小面积标准烫伤仪的实验应用[J]．中国临床康复，2002,6(20):3033

[3]陈晓东，施彦，杨荣华，等.异种(猪)脱细胞真皮基质对大鼠深Ⅱ度烧伤创面肿瘤坏死因子-α、白细胞介素-8、白细胞介素-1表达的影响[J].中华损伤与修复杂志（电子版）,2014，9(2):137

[4]Boyce S T．Design principles for composition and pedormance of cultured skin substitutes[J]．Bums，2001,27(5)：523

[5]周牮，李影学，迟云飞，等.早期应用异种脱细胞真皮基质修复小儿Ⅱ度烫伤创面的疗效观察[J].中华临床医师杂志,2015,9(8):1472

[6]宁勇,刘明锁,刘文文,等.异种脱细胞真皮基质覆盖自体微粒皮治疗大面积深度烧伤疗效观察[J].山东医药,2014,54(44):91

[7]梁黎明,柴家科,杨红明,等.激光微孔猪脱细胞真皮基质制备及创面移植的观察[J].中华烧伤杂志,2007,23(2):122

[8]Metcalfe A D，Fergnson M W．Harnessing wound healing and regeneration for tissue engineering[J]．Biochem Soc Trans,2005,33(Pt 2):413

[9]Wang Y, Mithieux S M, kong Y ，et a1.Tropoelastin incorporation into a dermal regeneration template promotes wound angiogenesis[J]. Adv Healthc Mater 2015,4(4):577

[10]辛国华,曾逃,方罗旭,等.微孔结构对脱细胞真皮基质胶原网架功能重建的影响[J]. 广东医学,2012,33(3):310

[11] Valarmathi M T, Davis J M, Yost M J, et a1. A three-dimensional model of vasculogenesis[J]．Biomaterials，2009,30(6):1098

[12] Nillesen S T, Geujes P J, Wismans R, et a1. Increased angiogenesis and blood vessel maturation in acellular collagen-heparin scarfolds containing both FGF2 and VEGF[J]. Biomatefials, 2007,28(6):1123

[13]徐少骏,马列,滕建英,等. 不同材料人工真皮支架修复猪Ⅲ度烧伤创面比较[J]. 中华整形外科杂志, 2010,26(5):360

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