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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

21卷

期数:

2015年03期

页码:

229-232

栏目:

基础医学

出版日期:

2015-05-20

文章信息/Info

Title:

 Effects of pioglitazone on the expression of TNF- α in kidney of obese mice

文章编号:

1006-8147（2015）03-0229-04

作者:

王安凤¹; 夏 天¹; 张秋平¹; 王永明²

(1．天津医科大学第二医院肾内科 ,天津300211 ; 2．天津动物实验中心 ,天津300192)

Author(s):

 WANG An-feng¹; XIA Tian ¹; ZHANG Qiu-ping ¹; WANG Yong-ming²

 (1．Department of Nephrology, The Second Hospital, Tianjin Medical University,Tianjin 300211, China；2. Animal Experiment Center of Tianjin，Tianjin 300192，China)

关键词:

肥胖相关性肾病; 肿瘤坏死因子-α; 吡格列酮; 尿微量白蛋白; 小鼠

Keywords:

				
				
				obesity related glomerulopathy; tumor necrosis factor-a; pioglitazone; uninary microalbumin; mice

分类号:

R692.9

DOI:

-

文献标志码:

A

摘要:

 目的：研究吡格列酮对肥胖小鼠肾脏中肿瘤坏死因子-α（TNF-α）表达的影响，探讨肥胖相关性肾病的防治意义。方法：选用OB小鼠和C57小鼠（作为对照组）进行对比观察，并将OB小鼠分为模型组和治疗组，治疗组给予吡格列酮灌胃，连续10周，实验前后测定小鼠血糖，尿微量白蛋白（mALB）。10周末处死小鼠，摘取双侧肾脏，免疫组化方法测定TNF-α表达水平，比较3组小鼠之间测定指标的差异，并进行相关性分析。结果：OB小鼠的mALB、TNF-α阳性百分比均明显高于C57小鼠，差异有统计学意义(P<0.01)；给予吡格列酮干预后，治疗组尿mALB、TNF-α阳性百分比均低于模型组，差异有统计学意义(P<0.01 )。结论：TNF-α在肥胖小鼠肾小管上皮细胞、肾小球系膜细胞的表达水平较普通小鼠明显升高，吡格列酮可以下调TNF-α在肥胖小鼠肾脏上的表达水平，从而减少尿mALB排泄。

Abstract:

				Objective: To study the effects of pioglitazone on the expression of tumor necrosis factor-a(TNF- α ) in the kidney of obese mice and to explore the prevention of obesity related glomerulopathy. Methods: The OB mice and C57 mice (as control group) were observed, and OB mice were divided into model group and treatment group, and the latter was given  pioglitazone orally for 10 weeks. before and after the experiment, blood glucose and uninary microalbumin (mALB) in mice were measured. Mice were sacrificed 1 at week 10, both kidneys were removed, and TNF-α expression level was determined by immunohistochemical method. The indicators were compared among 3 groups of mice, and the correlations were calculated. Results: OB mice urinary mALB and TNF- α positive percentage were significantly higher than those of C57 mice, (P<0.01); pioglitazone intervention treatment group, urinary mALB and TNF- α positive percentages were lower than those in the model group, and the difference was statistically significant (P<0.01). Conclusion: The expression level of TNF- alpha in obese mice renal tubular epithelial cells, mesangial cells significantly increased than normal mice and pioglitazone can lower the expression level of TNF- alpha in kidney of obese mice, thus reducing urinary albumin excretion.

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相似文献/References:

[1]戴一,姜铁民,高超,等.瑞舒伐他汀预处理影响小鼠心肌缺血再灌注炎症因子和细胞凋亡[J].天津医科大学学报,2013,19(05):380.
[2]李 颖 综述,夏 天 审校. 肥胖相关性肾病的研究进展[J].天津医科大学学报,2014,20(02):165.

备注/Memo

备注/Memo:

作者简介 王安凤（1989-），女，硕士在读，研究方向：内科学肾病；通信作者：夏天，E-mail:xiatian01@medmail.com.cn。

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更新日期/Last Update: 2015-05-27