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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

20卷

期数:

2014年04期

页码:

289-292

栏目:

临床医学

出版日期:

2014-07-08

文章信息/Info

Title:

Quantity chang and function of Th17 cells  in patients with immune thrombocytopenia

文章编号:

1006-8147（2014）04-0289-04

作者:

王丽静; 瞿文; 邵宗鸿; 阮二宝; 王晓明; 王国锦; 刘 鸿; 吴玉红; 宋 嘉; 邢莉民; 关 晶; 李丽娟; 王化泉

(天津医科大学总医院血液科，天津 300052)

Author(s):

WANG Li-jing;  QU Wen;  SHAO Zong-hong;  RUAN Er-bao;  WANG Xiao-ming;  WANG Guo-jin;  LIU Hong;  WU Yu-hong;  SONG Jia;  XING Li-min;  GUAN Jing;  LI Li-juan;  WANG Hua-quan

(Department of Hematology, General Hospital, Tianjin Medical University, Tianjin 300052, China)

关键词:

Th17细胞; 免疫性血小板减少症; 白介素17; 白介素23; 白介素17 mRNA

Keywords:

Th17 cells;  immune thrombocytopenia;  interleukin 17;  interleukin 23;  interleukin17 mRNA

分类号:

R558+.2

DOI:

-

文献标志码:

A

摘要:

目的：研究免疫性血小板减少症(ITP)患者Th17细胞数量、相关功能分子水平及其与临床指标的相关性，探讨Th17细胞在ITP发病机制中的作用。 方法：应用流式细胞术（FCM）检测骨髓IL-23R+CD4+/CD4+细胞比例；应用ELISA检测骨髓上清液IL-17、IL-23水平；RT-PCR测定骨髓单个核细胞中IL-17 mRNA的相对表达水平，并与临床指标进行相关性分析。 结果： ITP患者40例，正常对照组14名，ITP患者骨髓中IL-23R+ CD4+/CD4+细胞比例[(4.933±6.162)%]、 IL-17水平[(13.434±7.189)ng/L]、IL-23水平[(56.930±58.512)ng/L]、IL-17mRNA相对表达水平(0.921±0.196)均明显高于正常对照组[(0.897±0.803)%]、[(4.134±2.879 ng/L] 、[(17.841±11.033)ng/L] 、(0.749±0.136)(P<0.05)；活动期ITP患者IL-23R+ CD4+ /CD4+细胞比例[(6.699±7.008)%]、IL-17水平[(16.021±7.114) ng/L]、IL-23水平[(72.474±64.835) ng/L]、IL-17mRNA相对表达水平[0.967±0.186]均明显高于缓解期患者[(1.656±1.286)%]、[(7.972±3.326) ng/L]、[(24.114±17.654) ng/L)]、(0.801±0.163)(P<0.05)。ITP患者上述指标与其临床指标存在相关性。 结论：Th17细胞在ITP患者免疫发病机制中发挥重要作用，在ITP患者中不仅数量增加，而且功能活跃。

Abstract:

Objective: To study the quantity, related functional molecule level of Th17 cells and its correlation with clinical parameters in patients with immune thrombocytopenia, and to explore the role of Th17 cells in the immune pathogenesis. Methods: Forty patients were divided into two groups, including 26 ITP  patients in activity,14  patients in remission.There were 14 normal controls.The ratio of IL-23R⁺CD4⁺/ CD4⁺ cells in   bone marrow of patients were measured with flow cytometry , and the level of IL-17, IL-23 in bone marrow supernatant was obtained using enzyme-linked immunosorbent assay while the relative expression levels of IL-17 mRNA in bone marrow with reverse transcription-polymerase chain reaction technique. The correlation analysis on clinical indicators of ITP patients was made. Results: The ratio of IL-23R⁺CD4⁺/CD4⁺cells[(4.933±6.162)%], the level of IL-17 [(13.434±7.189)ng/L], IL-23[(56.930±58.512)ng/L] and IL-17 mRNA[0.921±0.196] in bone marrow with ITP patients were significantly higher than those of normal controls[(0.897±0.803)%],[(4.134±2.879 ng/L],[(17.841±11.033)ng/L],[0.749±0.136] (P<0.05). These four indicators in active ITP patients[(6.699±7.008)%], [(16.021±7.114) ng/L],[(72.474±64.835) ng/L],[0.967±0.186] were significantly higher than those in remission[(1.656±1.286)%],[(7.972±3.326) ng/L],[(24.114±17.654) ng/L)],[0.801±0.163] (P<0.05). The above parameters of ITP patients are correlated with clinical indicators. Conclusion: Th17 cells are very active with increased numbers in ITP patients , which plays an important role in the immune pathogenesis of ITP.

参考文献/References:

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备注/Memo

备注/Memo:

基金项目  天津市自然科学基金重点项目（12JCZDJC21500），卫生行业科研专项项目（201202017）

作者简介 王丽静(1987-),女，硕士在读，研究方向：血液病学；通信作者：瞿文，E-mail: quwentj923@ sina.com; 邵宗鸿，E-mail: shaozonghong@sina.com。

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更新日期/Last Update: 2014-06-06