|本期目录/Table of Contents|

[1]李长娥,于丹妮,韩育植,等.纳米银的制备及其对变异链球菌抗菌性的研究[J].天津医科大学学报,2014,20(03):220-223.
 LI Chang- e,YU Dan-ni,HAN Yu-zhi,et al.Research on the preparation of silver nanoparticles and its antibacterial property on Streptococcus mutans[J].Journal of Tianjin Medical University,2014,20(03):220-223.
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纳米银的制备及其对变异链球菌抗菌性的研究(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
20
期数:
2014年03期
页码:
220-223
栏目:
临床医学
出版日期:
2014-05-20

文章信息/Info

Title:
Research on the preparation of silver nanoparticles and its antibacterial property on Streptococcus mutans
文章编号:
1006-8147(2014)03-0220-04
作者:
李长娥于丹妮韩育植李 娜
(天津医科大学第二医院口腔科,天津300211)
Author(s):
LI Chang- e YU Dan-ni HAN Yu-zhi LI Na
(Department of Stomatology, The Second Hospital ,Tianjin Medical University, Tianjin 300211, China)
关键词:
葡萄糖纳米银变异链球菌抗菌性
Keywords:
glucosesilver nanoparticlesStreptococcus mutansantibacterial  property
分类号:
-
DOI:
-
文献标志码:
A
摘要:
目的:对纳米银的制备及其对变异链球菌标准株和耐氟株的抗菌性进行研究。方法:采用葡萄糖还原硝酸银制备纳米银;采用纸片扩散法和微稀释法测定该纳米银对标准株及耐氟株的抗菌效果。结果:制备的纳米银为分散性好、均粒径约为13.04 nm的类球形粒子,它对标准株及耐氟株的抑菌环直径分别为(10.87±0.67)mm、(9.68±0.86)mm,MIC为(2.08±0.90)?g/mL、(2.60±0.90) ?g/mL,MBC 为(4.16±1.81)?g/mL、(5.21±1.81)?g/mL。该纳米银对标准株和耐氟株均有抗菌性(P <0.05),且对两菌株抗菌性的差异无统计学意义(P >0.05)。结论:葡萄糖还原硝酸银可制备出对变异链球菌标准株及耐氟株有较好抗菌性的纳米银。
Abstract:
Objective:To explore the preparation of silver nanoparticles and its antibacterial property on Streptococcus mutans. Methods:Silver nanoparticles were prepared by reducing silver nitrate with glucose. Disk diffusion method and microdilution method were used to explore the antimicrobial properties of the silver nanoparticles on standard strain and fluoride–resistant strain of Streptococcus mutans. Results:The silver nanoparticles were spherical with 13.04 nm mean size and very well dispersed. The mean diameters of inhibition zone of the nanoparticles on test bacteria were(10.87±0.67) mm, (9.68±0.86) mm respectively while minimum inhibitory concentration were (2.08±0.90) ?g/mL, (2.60±0.90) ?g/mL and minimum bactericidal concentration were (4.16±1.81) ?g/mL, (5.21±1.81) ?g/mL. Silver nanoparticles had excellent antibacterial effect on the test bacteria, showing significant statistical differences(P <0.05). While the antibacterial property of silver nanoparticles on standard strain and fluoride–resistant strain revealed no significant difference(P >0.05). Conclusion:Glucose -reduced silver nitrate can be used prepare silver nanoparticles which has marked antibacterial on standard strain and fluoride–resistant strain of Streptococcus mutans.

参考文献/References:

[1] Chansley P E, Kral T A. Transformation of fluoride resistance genes in Streptococcus mutans[J]. Infect Immun, 1989,57(7):1968
[2] Bo L, Yang W, Chen M, et al. A simple and ‘green’ synthesis of polymer-based silver colloids and their antibacterial properties[J]. Chem Biodivers, 2009 ,6(1):111
[3] Rastogi S K, Rutledge V J, Gibson C, et al. Ag colloids and Ag clusters over EDAPTMS-coated silica nanoparticles: synthesis, characterization, and antibacterial activity against Escherichia coli[J]. Nanomedicine,2011 ,7(3):305
[4] 殷晓萍,于丹妮,陈锦英.变形链球菌耐氟株体外诱导分离方法的研究[J]. 天津医药, 2004,32(6):321
[5] Dasanayake A P, Caufield P W, Cutter G R,et al. Differences in the detection and enumeration of mutans streptococci due to differences in methods[J]. Arch Oral Biol, 1995,40(4):345
[6] Martínez-Castañón G A, Niño-Martínez N, Martínez-Gutierrez F, et al. Synthesis and antibacterial activity of silver nanoparticles with different sizes[J]. J Nanopart Res, 2008, 10(8):1343
[7] Espinosa-Cristóbal L F, Martínez-Castañón G A, Téllez-Déctor E J, et al. Adherence inhibition of Streptococcus mutans on dental enamel surface using silver nanoparticles[J].Mater Sci Eng C Mater Biol Appl, 2013 ,33(4):2197
[8] 熊金钰,徐国财. 纳米银的制备及表征[J]. 金属功能材料,2004,11(2):38
[9] Shameli K, Ahmad M B, Jazayeri S D, et al. Synthesis and characterization of polyethylene glycol mediated silver nanoparticles by the green method[J]. Int J Mol Sci, 2012,13(6):6639
[10] Jung W K, Koo H C, Kim K W, et al. Antibacterial activity and mechanism of action of the silver ion in Staphylococcus aureus and Escherichia coli [J]. Appl Env Microbiol, 2008,74 (7):2171
[11] Hernández-Sierra J F, Ruiz F, Pena D C, et al. The antimicrobial sensitivity of Streptococcus mutans to nanoparticles of silver, zinc oxide, and gold[J]. Nanomedicine, 2008,4(3):237
[12] Kim J S, Kuk E, Yu K N, et al. Antimicrobial effects of silver nanoparticles[J]. Nanomedicine,2007,3(1):95
[13] Chen M, Yang Z, Wu H, et al. Antimicrobial activity and the mechanism of silver nanoparticle thermosensitive gel[J]. Int J Nanomedicine, 2011,6:2873
[14] Cheng L, Zhang K, Weir M D, et al. Effects of antibacterial primers with quaternary ammonium and nano-silver on Streptococcus mutans impregnated in human dentin blocks[J].Dent Mater, 2013 ,29(4):462
[15] Fayaz A M, Balaji K, Girilal M, et al. Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: a study against gram-positive and gram-negative bacteria[J]. Nanomedicine, 2010 ,6(1):103 

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备注/Memo

备注/Memo:
作者简介 李长娥(1987-),女,硕士在读,研究方向:口腔内科学;通信作者:于丹妮,E-mail:danni2468@eyou.com。
更新日期/Last Update: 2014-05-12