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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

20卷

期数:

2014年03期

页码:

188-191

栏目:

基础医学

出版日期:

2014-05-20

文章信息/Info

Title:

Bioinformation study of capsid protein Vpl high conservative region parts from Chlamydiaphage

文章编号:

1006-8147(2014)03-0188-04

作者:

姚卫锋¹; 卢桂玲¹; 谢艳秋¹; 于 旺¹; 宋蒙蒙¹; 李士颖²

(1. 天津中医药研究院附属医院皮肤科,天津 300120;2. 天津公安医院内三科,天津 300042)

Author(s):

YAO Wei-feng¹ ; LU Gui-ling¹; XIE Yan-qiu¹; YU Wang¹; SONG Meng-meng¹; LI Shi-ying²

(1. Department of Dermatology，Tianjin Academy of Traditional Chinese Medicine Affiliated Hospital,Tianjin 300120,China;2. Internal Medicine3, Tianjin Gongan Hospital,Tianjin 300042,China)

关键词:

微病毒; 蛋白; 结构; 分析

Keywords:

microvirus;  protein;  structure;  analysis

分类号:

-

DOI:

-

文献标志码:

A

摘要:

 目的：分析衣原体噬菌体Vpl蛋白及其高保守区的二级结构并预测其B细胞表位。方法：以ClustalX程序比对各株衣原体噬菌体衣壳蛋白Vp1序列获得高保守区序列。以Vp1氨基酸序列为基础，采用Gamier-Robson法、Chou-Fasman法、Eisenberg法和Karplus-Schulz法分析蛋白二级结构；按Kyte-Doolittle法、Emini法和Jameson-Wolf法预测蛋白的抗原表位。结果：衣原体噬菌体Vpl蛋白的二级结构以β折叠为主，有少量α螺旋；其高保守区含多个抗原位点，预测其N端1-8,103-110,158-164, 189-196,322-332, 427-434,478-488为优势表位。结论：衣原体噬菌体Vpl蛋白及其高保守区存在复杂的蛋白结构，可形成多个可选表位。

Abstract:

Objective: To predict the secondary structure and B cell epitope of capsid protein Vp l in high conservative region． Methods: The conservative region was acquired by comparing the Vp1 protein sequences. The secondary structure of these regions were predicted by the method of Gamier-Robson，Chou-Fasman, Eisenberg and Karplus-Schulz while its cell epitope was predicted by the method of Kyte-Doolittle，Emini and Jameson-Wolf．Results: The main constructions of Chlamydiaphage Vp1 were β regions, including several α regions. And the sections of1-8,103-110,158-164, 189-196,322-332, 427-434,478-488 in the N-terminal of the conservative region could be the epitopes of B cel1．Conclusion: The conservative region of Vpl protein has the complicated structures and can form into multiple epitopes．

参考文献/References:

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[7] Larsen J E, Lund O, Nielsen M.Improved method for predieting linear B-cell epitopes[J].Immunome Res,2006,2(2):1
[8] Beaver J E, Bourne P E, Ponomarenko J V. EpitopeViewer: a Java application for the visualization and analysis of immune epitopes in the Immune Epitope Database and Analysis Resource [ J ] . Immunome Res, 2007,21(2): 3
[9] Yoo S Y, Merzlyak A, Chung W J,et al.Facile growth factor immobilization platform based on engineered phage matrices[J]. Soft Matter,2011,14(7):1660
[10] 郑小莉,张艺, 谢晓东,等.鼠Minele蛋白B细胞抗原表位预测及多克隆抗体制备[J].免疫学杂志,2011,27(8):683
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备注/Memo

备注/Memo:

作者简介 姚卫锋（1977-），男，主治医师，博士，研究方向：皮肤病学；通信作者：李士颖，E-mail:shiying7803@163.com。

常用功能

更新日期/Last Update: 2014-05-12