|本期目录/Table of Contents|

[1]董莉真,吴志敏,朱 泽,等. 人参皂苷Rg3调控ERK通路抑制Lewis小鼠肺癌生长的研究[J].天津医科大学学报,2014,20(04):271-274.
 DONG Li-zhen,WU Zhi-min,ZHU Ze,et al.Ginsenoside Rg3 inhibitions Lewis lung carcinoma growth by mediating ERK pathway[J].Journal of Tianjin Medical University,2014,20(04):271-274.
点击复制

 人参皂苷Rg3调控ERK通路抑制Lewis小鼠肺癌生长的研究(PDF)
分享到:

《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
20卷
期数:
2014年04期
页码:
271-274
栏目:
基础医学
出版日期:
2014-07-08

文章信息/Info

Title:
Ginsenoside Rg3 inhibitions Lewis lung carcinoma growth by mediating ERK pathway
文章编号:
1006-8147(2014)04-0271-04
作者:
董莉真吴志敏朱 泽李光明
(天津医科大学病原生物学教研室,天津 300070)
Author(s):
DONG Li-zhen WU Zhi-min ZHU Ze LI Guang-ming
(Department of Microbiology, Tianjin Medical University, Tianjin 300070, China)
关键词:
人参皂苷Rg3ERKDUSP6 Lewis 肺癌小鼠
Keywords:
GS-Rg3 ERKDusp6 Lewis lung carcinoma mice
分类号:
R734.2
DOI:
-
文献标志码:
A
摘要:
目的:通过人参皂苷Rg3对Lewis肺癌小鼠模型及其细胞模型的抑制研究,探讨ERK信号通路及相关基因在抗肿瘤中的作用。方法:建立Lewis肺癌小鼠荷瘤动物模型,通过口饲法给予定量人参皂苷,连续3周,处死动物后,比较各组荷瘤小鼠肿瘤体积。含药动物血清作用于Lewis肺癌细胞,MTT法检测各组细胞抑制效率。Realtime-PCR法检测荷瘤鼠Dusp6,Bax,Bcl-2基因表达变化,Western blot法检测荷瘤鼠ERK,p-ERK蛋白表达变化。结果:人参皂苷Rg3对Lewis肺癌细胞及荷瘤小鼠的肿瘤生长均有明显抑制作用(P <0.01)。Western blot显示ERK在Rg3处理组中的表达明显比对照组低,Realtime-PCR结果显示,在动物肿瘤标本中,Dusp6,Bax基因药物处理组比对照组高表达(P <0.01),Bcl-2基因药物处理组比对照组低表达(P <0.01)。结论:人参皂苷Rg3在Lewis肺癌体内外模型中具有一定的肿瘤抑制效果,ERK信号通路及Dusp6基因与Rg3的抑制作用有关,Rg3诱导的细胞凋亡也参与了抗肿瘤的作用。
Abstract:
Objective: To discuss the ERK pathway and its relative geneticfunction in Lewis lung carcinoma animal and cell model through Ginsenoside Rg3 treatment. Methods: The Lewis lung carcinoma mice model was established after feeding Rg3 for 3 weeks, and then the volumes of the tumor were calculated. The inhibition effect on Lewis lung carcinoma cell line was detected by MTT assay. The changes in Dusp6, Bax, Bcl-2 expressions in tumor model were detected by Realtime-PCR.The changes in ERK and p-ERK protein expression were determined by western blot. Results: Ginsenoside Rg3 could inhibit Lewis lung carcinoma cell and animal model (P<0.01).ERK protein expression was significantly decreased in Rg3 group compared with control group by western blot assay. Dusp6 and Bax were over expressed in Rg3 group compared with control(P <0.01)while the Bcl-2 was under expressed(P <0.01). Conclusion: Ginsenoside Rg3 exhibits certain inhibition ability in both invivo and invitro model. ERK pathway and Dusp6 gene are associated with the Rg3 inhibition effect, and the apoptosis effect induced by rg3 is also involved in the inhibition.

参考文献/References:

[1]Liu T G, Huang Y, Cui D D, et al. Inhibitory effect of ginsenoside Rg3 combined with gemcitabine on angiogenesis and growth of lung Cancer in mice[J]. BMC Cancer, 2009,9(1):250
[2]Kim B J, Nah S Y, Jeon J H, et al. Transient receptor potential melastatin 7 channels are involved in ginsenoside Rg3-induced apoptosis in gastric Cancer cells[J]. Basic Clin Pharmacol Toxicol, 2011,109(4):233
[3]Jiang J W, Chen X M, Chen X H, et al. Ginsenoside Rg3 inhibit hepatocellular carcinoma growth via intrinsic apoptotic pathway[J]. World J Gastroenterol, 2011,17(31):3605 [4]Hu S S, Zhou L K, Ba Y, et al. A meta-analysis of ginsenoside Rg3 for non-small cell lung Cancer[J]. Clinical Oncology and Cancer Research, 2011,8(3):175
[5]Chen X P, Qian L L, Jiang H, et al. Ginsenoside Rg3 inhibits CXCR4 expression and related migrations in a breast Cancer cell line[J]. Int J Clin Oncol, 2011,16(5):519
[6]Gao J L, Lv G Y, He B C, et al. Ginseng saponin metabolite 20(S)-protopanaxadiol inhibits tumor growth by targeting multiple Cancer signaling pathways[J]. Oncol Rep, 2013,30(1):292
[7]Lu P, Su W, Miao Z H, et al. Effect and mechanism of ginsenoside Rg3 on postoperative Life span of patients with non-small cell lung Cancer[J]. Chin J Integr Med, 2008,14(1):33
[8]Chen Q J, Zhang M Z, Wang L X. Gensenoside Rg3 inhibits hypoxia-induced VEGF expression in human Cancer cells[J]. Cell Physiol Biochem, 2010,26(6):849
[9]Choi Y J, Lee H J, Kang D W, et al. Ginsenoside Rg3 induces apoptosis in the U87MG human glioblastoma cell line through the MEK signaling pathway and reactive Oxygen species[J]. Oncol Rep, 2013,30(3):1362
[10]Jeffrey K L, Camps M, Rommel C, et al. Targeting dual-specificity phosphatases: manipulating MAP kinase signalling and immune responses[J]. Nat Rev Drug Discov, 2007,6(5):391
[11]Wu J J, Zhang L, Bennett A M. The noncatalytic amino terminus of mitogen-activated protein kinase phosphatase 1 directs nuclear targeting and serum response element transcriptional regulation[J]. Mol Cell Biol, 2005,25(11):4792
[12]Masuda K, Shima H, Watanabe M, et al. MKP-7, a novel mitogen-activated protein kinase phosphatase, functions as a shuttle protein[J]. J Biol Chem, 2001,276(42):39002 [13]Chan D W, Liu V W, Tsao G S, et al. Loss of MKP3 mediated by oxidative stress enhances tumorigenicity and chemoresistance of ovarian Cancer cells[J]. Carcinogenesis, 2008,29(9):1742
[14]Zhang Z, Kobayashi S, Borczuk A C, et al. Dual specificity phosphatase 6 (DUSP6) is an ETS-regulated negative feedback mediator of oncogenic ERK signaling in lung Cancer cells[J]. Carcinogenesis, 2010,31(4):577
[15]Ma J, Yu X, Guo L, et al. DUSP6, a tumor suppressor, is involved in differentiation and apoptosis in esophageal squamous cell carcinoma[J]. Oncol Lett, 2013,6(6):1624

相似文献/References:

[1]王超坤,刘婷婷,帅一尘,等.基于RNA-seq探讨扶肾降浊方及蚓激酶对肾间质纤维化的作用机制[J].天津医科大学学报,2023,29(02):153.
 WANG Chao-kun,LIU Ting-ting,SHUAI Yi-chen,et al.Mechanism of FushenJiangzhuo recipe and lumbrokinase on renal interstitial fibrosis based on RNA-seq[J].Journal of Tianjin Medical University,2023,29(04):153.
[2]吴疆,赵斌,陈啸,等.关节镜联合胫骨高位截骨术促进膝关节内侧间室骨性关节炎软骨再生的机制研究[J].天津医科大学学报,2023,29(03):317.
 WU Jiang,ZHAO Bin,CHEN Xiao,et al.Mechanism of arthroscopy combined with high tibial osteotomy inpromoting cartilage regeneration in medial compartment of knee osteoarthritis[J].Journal of Tianjin Medical University,2023,29(04):317.

备注/Memo

备注/Memo:
基金项目 天津医科大学基金资助项目(2013KYQ13)
作者简介 董莉真(1988-),女,硕士在读,研究方向:肺癌发生的分子机制;通信作者:朱泽,E-mail:zhuze@tijmu.edu.cn,李光明,E-mail: li-guangming@hotmail.com 。
更新日期/Last Update: 2014-06-06