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[1]杨传慧,李 萍,何 庆,等.不同时间持续缺氧对3T3-L1脂肪细胞脂肪因子基因表达的影响[J].天津医科大学学报,2015,21(03):1-5.
 YANG Chuan-hui,LI Ping,HE Qing,et al.Effect of incubation time of hypoxia on the levels of adipokines in the 3T3-L1 adipocytes[J].Journal of Tianjin Medical University,2015,21(03):1-5.
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不同时间持续缺氧对3T3-L1脂肪细胞脂肪因子基因表达的影响(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
21
期数:
2015年03期
页码:
1-5
栏目:
基础医学
出版日期:
2015-05-20

文章信息/Info

Title:
Effect of incubation time of hypoxia on the levels of adipokines in the 3T3-L1 adipocytes
文章编号:
1006-8147(2015)0-0001-05
作者:
杨传慧1李 萍1何 庆1刘金泉2牛文彦2
(1.天津医科大学总医院内分泌科,天津 300052;2 .天津医科大学免疫学系,天津 300070)
Author(s):
YANG Chuan-hui1 LI Ping1 HE Qing1 LIU Jin-quan2 NIU Wen-yan2
(1. Department of Endocrinology, General Hospital ,Tianjin Medical University,Tianjin 300052,China; 2. Department of Immunology, Tianjin Medical University, Tianjin 300070, China)
关键词:
脂肪细胞缺氧诱导因子1α葡萄糖转运子1缺氧脂联素瘦素肥胖胰岛素抵抗
Keywords:
adipocytes hypoxia hypoxia inducible factor-1 glucose transporter 1adiponectin leptin obesity insulin resistance
分类号:
R589.2
DOI:
-
文献标志码:
A
摘要:
  目的:探讨不同时间持续缺氧孵育后脂肪细胞脂联素和瘦素表达的变化。方法:将分化成熟的3T3-L1脂肪细胞随机分为6组,即常氧(21%O2)和缺氧1%O2)4、12、24、48、72 h组,实时定量PCR(qRT-PCR)法测定各时间点脂肪细胞中HIF-1α、GLUT-1、脂联素和瘦素基因表达。结果:与常氧组相比,缺氧12 、24 、48 h组HIF-1α、GLUT-1 mRNA表达水平升高,差异有统计学意义(P<0.05)。缺氧24 h时HIF-1α、GLUT-1 mRNA达到最高。各缺氧组脂联素mRNA在12、24、48、72 h缺氧组的表达下降,较常氧组有统计学差异(P<0.05)。各缺氧组瘦素mRNA的表达高于常氧,并在24 h时达到最高,差异有统计学意义(P<0.05)。结论:缺氧使脂肪细胞HIF-1α、GLUT-1、瘦素mRNA表达升高,脂联素mRNA表达下降,各反应在缺氧24 h时达到最大水平。
Abstract:
Objective: To explore the effect of different incubation time of hypoxia on the expression of adipokines in the 3T3-L1adipocytes. Methods: The differentiated 3T3-L1 adipocytes were randomly divided into 6 groups, normoxia(21%O2)and hypoxia(1%O2)conditions for 4, 12, 2, 48, 72 h respectively. The mRNA expressions of hypoxia inducible factor-1α (HIF-1α), glucose transporter 1 (GLUT1), adiponectin and leptin were detected by the qRT-PCR. Results: In 3T3-L1 adipocytes, the mRNA expressions of HIF-1α and GLUT1 in hypoxia conditions for 12 h , 24 h and 48 h were higher than normoxia-treated cells (P<0.05); furthermore, they were highest in the group of hypoxia–treated cells for 24 h. Compared with the normoxia group, the adiponectin mRNA expressions of the hypoxia–treated 3T3-L1 adipocytes decreased in all groups (P<0.05) except for the 4 h group (P<0.05). Ttranscription level of leptin in the hypoxia-treated cells increased(P<0.05), and it reached the highest level in the 24 h hypoxia group. Conclusion: The mRNA expressions of HIF-1α, GLUT1 and leptin of 3T3-L1 adipocytes increase in the condition of hypoxia incubation, while the level of adiponectin mRNA decreases under hypoxia treatment. All changes above reach the peaks within the 24 hours of hypoxia-treated 3T3-L1 adipocytes.

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[2]杨传慧,李 萍,何 庆,等.不同时间持续缺氧对3T3-L1脂肪细胞脂肪因子基因表达的影响[J].天津医科大学学报,2015,21(01):1.
 YANG Chuan-hui,LI Ping,HE Qing,et al. Effect of incubation time of hypoxia on the expression of adipokines in the 3T3-L1 adipocytes[J].Journal of Tianjin Medical University,2015,21(03):1.

备注/Memo

备注/Memo:

基金项目 国家自然科学基金资助项目(81170740, 81270144);天津市科委科技支撑项目(09ZCZDSF04500)

作者简介 杨传慧(1988-),女,硕士在读,研究方向:内分泌与代谢;通信作者:何庆,E-mail:hech69@hotmail.com

更新日期/Last Update: 2015-01-22