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[1]杨传慧,李 萍,何 庆,等.不同时间持续缺氧对3T3-L1脂肪细胞脂肪因子基因表达的影响[J].天津医科大学学报,2015,21(03):1-5.
　YANG Chuan-hui,LI Ping,HE Qing,et al.Effect of incubation time of hypoxia on the levels of adipokines in the 3T3-L1 adipocytes[J].Journal of Tianjin Medical University,2015,21(03):1-5.
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不同时间持续缺氧对3T3-L1脂肪细胞脂肪因子基因表达的影响(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
21卷
期数:
2015年03期
页码:
1-5
栏目:
基础医学
出版日期:
2015-05-20

文章信息/Info

Title:
Effect of incubation time of hypoxia on the levels of adipokines in the 3T3-L1 adipocytes
文章编号:
1006-8147（2015）0-0001-05
作者:
杨传慧1; 李 萍1; 何 庆1; 刘金泉2; 牛文彦2
(1.天津医科大学总医院内分泌科,天津 300052;2 .天津医科大学免疫学系,天津 300070)
Author(s):
YANG Chuan-hui1;  LI Ping1;  HE Qing1;  LIU Jin-quan2;  NIU Wen-yan2
(1. Department of Endocrinology, General Hospital ,Tianjin Medical University,Tianjin 300052,China; 2. Department of Immunology, Tianjin Medical University, Tianjin 300070, China)
关键词:
脂肪细胞; 缺氧诱导因子1α; 葡萄糖转运子1; 缺氧; 脂联素; 瘦素; 肥胖; 胰岛素抵抗
Keywords:
adipocytes;  hypoxia;  hypoxia inducible factor-1;  glucose transporter 1; adiponectin;  leptin;  obesity;  insulin resistance
分类号:
R589.2
DOI:
-
文献标志码:
A
摘要:
  目的：探讨不同时间持续缺氧孵育后脂肪细胞脂联素和瘦素表达的变化。方法：将分化成熟的3T3-L1脂肪细胞随机分为6组，即常氧（21%O2）和缺氧（1%O2）4、12、24、48、72 h组，实时定量PCR（qRT-PCR）法测定各时间点脂肪细胞中HIF-1α、GLUT-1、脂联素和瘦素基因表达。结果：与常氧组相比，缺氧12 、24 、48 h组HIF-1α、GLUT-1 mRNA表达水平升高，差异有统计学意义（P＜0.05）。缺氧24 h时HIF-1α、GLUT-1 mRNA达到最高。各缺氧组脂联素mRNA在12、24、48、72 h缺氧组的表达下降，较常氧组有统计学差异（P＜0.05）。各缺氧组瘦素mRNA的表达高于常氧，并在24 h时达到最高，差异有统计学意义（P＜0.05）。结论：缺氧使脂肪细胞HIF-1α、GLUT-1、瘦素mRNA表达升高，脂联素mRNA表达下降，各反应在缺氧24 h时达到最大水平。
Abstract:
Objective: To explore the effect of different incubation time of hypoxia on the expression of adipokines in the 3T3-L1adipocytes. Methods: The differentiated 3T3-L1 adipocytes were randomly divided into 6 groups, normoxia（21%O2）and hypoxia（1%O2）conditions for 4, 12, 2, 48, 72 h respectively. The mRNA expressions of hypoxia inducible factor-1α (HIF-1α), glucose transporter 1 (GLUT1), adiponectin and leptin were detected by the qRT-PCR. Results: In 3T3-L1 adipocytes, the mRNA expressions of HIF-1α and GLUT1 in hypoxia conditions for 12 h , 24 h and 48 h were higher than normoxia-treated cells (P＜0.05); furthermore, they were highest in the group of hypoxia–treated cells for 24 h. Compared with the normoxia group, the adiponectin mRNA expressions of the hypoxia–treated 3T3-L1 adipocytes decreased in all groups (P＜0.05) except for the 4 h group (P＜0.05). Ttranscription level of leptin in the hypoxia-treated cells increased(P＜0.05), and it reached the highest level in the 24 h hypoxia group. Conclusion: The mRNA expressions of HIF-1α, GLUT1 and leptin of 3T3-L1 adipocytes increase in the condition of hypoxia incubation, while the level of adiponectin mRNA decreases under hypoxia treatment. All changes above reach the peaks within the 24 hours of hypoxia-treated 3T3-L1 adipocytes.

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[4]杨传慧,李 萍,何 庆,等.不同时间持续缺氧对3T3-L1脂肪细胞脂肪因子基因表达的影响[J].天津医科大学学报,2015,21(01):1.
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备注/Memo

备注/Memo:

基金项目 国家自然科学基金资助项目（81170740, 81270144）；天津市科委科技支撑项目（09ZCZDSF04500）

作者简介 杨传慧(1988-)，女，硕士在读，研究方向：内分泌与代谢；通信作者：何庆，E-mail：hech69@hotmail.com 。

更新日期/Last Update: 2015-01-22