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[1]程 帅,赵淑荣,崔彭飞,等.聚乙烯基萘纳米微球制备及在胶乳增强免疫比浊法检测中的应用[J].天津医科大学学报,2014,20(06):441-444.
 CHENG Shuai,ZHAO Shu-rong,CUI Peng-fei,et al.Preparation of polyvinyl naphthalene nanopaticles and its application in LETIA[J].Journal of Tianjin Medical University,2014,20(06):441-444.
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聚乙烯基萘纳米微球制备及在胶乳增强免疫比浊法检测中的应用

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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
20卷
期数:
2014年06期
页码:
441-444
栏目:
基础医学
出版日期:
2014-11-20

文章信息/Info

Title:
Preparation of polyvinyl naphthalene nanopaticles  and its application in LETIA
文章编号:
1006-8147(2014)06-0441-04
作者:
 程 帅1赵淑荣2崔彭飞1王冬梅1徐 亮1杨志民1
(1.天津医科大学药学院, 天津市临床药物关键技术重点实验室,天津300070 ; 2.巴彦淖尔市医院护理部,巴彦淖尔 015100)
Author(s):
 CHENG Shuai1ZHAO Shu-rong2CUI Peng-fei1WANG Dong-mei1XU Liang1YANG Zhi-min1
(1. School of Pharmacy, Tianjin Medical University, Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics) , Tianjin 300070, China; 2. Department of Nursing , Bayan’nao’er Hospital, Bayan’nao’er 015100, China)
关键词:
聚乙烯基萘纳米微球胶乳增强免疫比浊法β2微球蛋白物理吸附
Keywords:
polyvinyl naphthalene nanoparticles latex-enhanced turbidimetric immunoassay β2-macroglobulin physical absorbance
分类号:
R914+R446
DOI:
-
文献标志码:
A
摘要:
 目的: 采用乳液聚合法制备聚乙烯基萘(PVN)纳米微球,将其用作β2微球蛋白(β2-M)胶乳增强免疫比浊(LETIA)检测试剂的新型载体。方法: 以乙烯基萘(VN)为聚合单体,十二烷基硫酸钠(SDS)为乳化剂,碳酸盐缓冲液为水相,制备了PVN纳米微球,并对微球的理化性能进行了表征。以物理吸附的方式分别将β2-M的抗体(IgG)连接到PVN及聚苯乙烯(PS)微球表面,制备得到用于β2-M检测的两种LETIA试剂,并在生化分析仪上对试剂的性能进行了评价。结果: 制备的PVN纳米微球具有均一的粒径分布。利用自制的以PVN纳米微球为载体的LETIA免疫检测试剂在一定范围内有较好线性,同时与自制的以同等粒径PS纳米微球为载体的LETIA检测试剂相比具有更高灵敏度。结论: PVN纳米微球作为LETIA检测试剂的载体具有很好应用前景。
Abstract:
 Objective: To prepare polyvinyl naphthalene (PVN) nanospheres and apply it in latex-enhanced turbidimetric immunoassay (LETIA) of beta 2 microglobin (β2-M). Methods: PVN nanospheres were prepared by emulsion polymerization. Vinylnaphthalene, sodium dodecyl sulfate and carbonate buffer were applied as polymerizable monomer, emulsifier and aqueous phase, respectively. The physicochemical properties of PVN nanoparticles were identified. In addition, the anti-β2-M antibodies were immobilized on the surface of the PVN and polystyrene (PS) nanopariticles by physical absorption. The reagents were then evaluated by a biochemical analyzer. Results: The PVN nanoparticles were in identical size distribution. Compared with the LETIA reagent prepared with PS nanoparticles, the LETIA reagent with self-manufactured PVN nanoparticls manifested broader linear range and higher sensitivity. Conclusion: PVN nanoparticles can function efficiently as novel carriers of LETIA reagent.

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备注/Memo

备注/Memo:
基金项目 天津市应用基础与前沿技术研究计划项目(14JCYBJC24300)
作者简介 程帅(1990-),女,硕士在读,研究方向:有机高分子微球的研制;通信作者:徐亮,E-mail: xuliang@tijmu.edu.cn
更新日期/Last Update: 2014-12-02