[1]程 帅,赵淑荣,崔彭飞,等.聚乙烯基萘纳米微球制备及在胶乳增强免疫比浊法检测中的应用[J].天津医科大学学报,2014,20(06):441-444.
CHENG Shuai,ZHAO Shu-rong,CUI Peng-fei,et al.Preparation of polyvinyl naphthalene nanopaticles and its application in LETIA[J].Journal of Tianjin Medical University,2014,20(06):441-444.
点击复制
聚乙烯基萘纳米微球制备及在胶乳增强免疫比浊法检测中的应用
(PDF)
《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]
- 卷:
-
20卷
- 期数:
-
2014年06期
- 页码:
-
441-444
- 栏目:
-
基础医学
- 出版日期:
-
2014-11-20
文章信息/Info
- Title:
-
Preparation of polyvinyl naphthalene nanopaticles and its application in LETIA
- 文章编号:
-
1006-8147(2014)06-0441-04
- 作者:
-
程 帅1; 赵淑荣2; 崔彭飞1; 王冬梅1; 徐 亮1; 杨志民1
-
(1.天津医科大学药学院, 天津市临床药物关键技术重点实验室,天津300070 ; 2.巴彦淖尔市医院护理部,巴彦淖尔 015100)
- Author(s):
-
CHENG Shuai1; ZHAO Shu-rong2; CUI Peng-fei1; WANG Dong-mei1; XU Liang1; YANG Zhi-min1
-
(1. School of Pharmacy, Tianjin Medical University, Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics) , Tianjin 300070, China; 2. Department of Nursing , Bayan’nao’er Hospital, Bayan’nao’er 015100, China)
-
- 关键词:
-
聚乙烯基萘纳米微球; 胶乳增强免疫比浊法; β2微球蛋白; 物理吸附
- Keywords:
-
polyvinyl naphthalene nanoparticles; latex-enhanced turbidimetric immunoassay; β2-macroglobulin; physical absorbance
- 分类号:
-
R914+R446
- DOI:
-
-
- 文献标志码:
-
A
- 摘要:
-
目的: 采用乳液聚合法制备聚乙烯基萘(PVN)纳米微球,将其用作β2微球蛋白(β2-M)胶乳增强免疫比浊(LETIA)检测试剂的新型载体。方法: 以乙烯基萘(VN)为聚合单体,十二烷基硫酸钠(SDS)为乳化剂,碳酸盐缓冲液为水相,制备了PVN纳米微球,并对微球的理化性能进行了表征。以物理吸附的方式分别将β2-M的抗体(IgG)连接到PVN及聚苯乙烯(PS)微球表面,制备得到用于β2-M检测的两种LETIA试剂,并在生化分析仪上对试剂的性能进行了评价。结果: 制备的PVN纳米微球具有均一的粒径分布。利用自制的以PVN纳米微球为载体的LETIA免疫检测试剂在一定范围内有较好线性,同时与自制的以同等粒径PS纳米微球为载体的LETIA检测试剂相比具有更高灵敏度。结论: PVN纳米微球作为LETIA检测试剂的载体具有很好应用前景。
- Abstract:
-
Objective: To prepare polyvinyl naphthalene (PVN) nanospheres and apply it in latex-enhanced turbidimetric immunoassay (LETIA) of beta 2 microglobin (β2-M). Methods: PVN nanospheres were prepared by emulsion polymerization. Vinylnaphthalene, sodium dodecyl sulfate and carbonate buffer were applied as polymerizable monomer, emulsifier and aqueous phase, respectively. The physicochemical properties of PVN nanoparticles were identified. In addition, the anti-β2-M antibodies were immobilized on the surface of the PVN and polystyrene (PS) nanopariticles by physical absorption. The reagents were then evaluated by a biochemical analyzer. Results: The PVN nanoparticles were in identical size distribution. Compared with the LETIA reagent prepared with PS nanoparticles, the LETIA reagent with self-manufactured PVN nanoparticls manifested broader linear range and higher sensitivity. Conclusion: PVN nanoparticles can function efficiently as novel carriers of LETIA reagent.
参考文献/References:
[1]池继敏,张峰,张静.不同类型关节炎患者血液及关节液中尿酸、抗链球菌溶血素O和类风湿因子含量比较[J].中华临床医师杂志:电子版,2013,7(21):9557
[2]黄志伟,沈均南,陈文.胶乳增强免疫比浊法试剂测定β2-微球蛋白的临床使用评价[J].标记免疫分析与临床,2007,14(2):117
[3]李新星,杨泽松,罗效梅.血清及尿液β2微球蛋白检测在糖尿病肾病分期中的诊断价值[J].国际检验医学杂志,2013,34(24):3431
[4]瓯阳涓.姜傥.肾的损伤性诊断[J].中华检验医学杂志,2005,28(8):877
[5]Spadaro A, Tortorella V, Morace C, et al. High circulating D-dimers are associated with ascites and hepatocellular carcinoma in liver cirrhosis[J]. World J Gastroenterol, 2008, 14(10): 1549
[6]亓春玲.D-二聚体与CEA、CA15-3在乳腺癌新辅助化疗中的临床检测分析[J].社区医学杂志,2013,11(18):20
[7]张利亚,李岩,汤继宏.多发性抽动症患儿血清抗DNA酶B抗体、抗链球菌溶血素O和C反应蛋白的测定及其临床意义[J].苏州大学学报:医学版,2010,30(2):438
[8]吴劲松.胶乳增强免疫透射比浊法测定CRP的方法学评价[J].放射免疫学杂志,2010,23(6):686
[9]Le Bricon T, Thervet E, Froissart M, et al. Plasma cystatin C is superior to 24-h creatinine clearance and plasma creatinine for estimation of glomerular filtration rate 3 months after kidney transplantation[J]. Clin Chem, 2000, 46(8 Pt 1): 1206
[10]王定保,秦娜.两种液相免疫比浊法测定胱抑素C的试剂性能平行比对[J].中国卫生检验杂志,2013,23(14):2888
[11]陈建文,张凡,刘玉侠,等.乳胶增强免疫比浊法测定血清肌钙蛋白I的实验探讨[J].中华全科医学,2013,11(11):1777
[12]林天浩,莫恒勤.免疫比浊法检测糖化血红蛋白的性能评价[J].现代医院,2013,13(11):60
[13]吴宁梅.胶乳增强免疫比浊法测定血清IgE[J].中国当代医药,2010,17(17):84
[14]邱志琦,温少磊,谭智毅.胃蛋白酶原Ⅰ、Ⅱ及其比值在胃癌筛查中的应用[J].检验医学与临床,2013,10(14):1806
[15]曹同玉,刘庆普,胡金生.聚合物乳液合成原理性能及应用[M].第2版.北京:化学工业出版社,2007:120-120
相似文献/References:
备注/Memo
- 备注/Memo:
-
基金项目 天津市应用基础与前沿技术研究计划项目(14JCYBJC24300)
作者简介 程帅(1990-),女,硕士在读,研究方向:有机高分子微球的研制;通信作者:徐亮,E-mail: xuliang@tijmu.edu.cn。
更新日期/Last Update:
2014-12-02