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[1]梁静静,刘铠瑞,王佳文,等.Ezrin影响PDCs侵袭和迁移的初步研究[J].天津医科大学学报,2026,32(04):371-377.[doi:10.20135/j.issn.1006-8147.2026.04.0371]
　LIANG Jingjing,LIU Kairui,WANG Jiawen,et al.Preliminary study on the influence of Ezrin on the invasion and migration of PDCs[J].Journal of Tianjin Medical University,2026,32(04):371-377.[doi:10.20135/j.issn.1006-8147.2026.04.0371]
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Ezrin影响PDCs侵袭和迁移的初步研究(PDF)

《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
32卷
期数:
2026年04期
页码:
371-377
栏目:
论著
出版日期:
2026-07-10

文章信息/Info

Title:
Preliminary study on the influence of Ezrin on the invasion and migration of PDCs
文章编号:
1006-8147（2026）04-0371-07
作者:
梁静静1; 刘铠瑞1; 王佳文1; 2; 王婧1; 张诗武1; 2
（1.天津医科大学研究生院，天津300070；2.天津市人民医院病理科，天津300121）
Author(s):
LIANG Jingjing1; LIU Kairui1; WANG Jiawen1; 2; WANG Jing1; ZHANG Shiwu1; 2
（1.Graduate School， Tianjin Medical University， Tianjin 300070， China； 2.Department of Pathology， Tianjin Union Medical Center， Tianjin 300121， China）
关键词:
Ezrin; 多倍体肿瘤巨细胞; 上皮-间充质转化; 侵袭; 迁移
Keywords:
Ezrin; polyploid giant cancer cells; epithelial-mesenchymal transition; invasion; migration
分类号:
R737.9
DOI:
10.20135/j.issn.1006-8147.2026.04.0371
文献标志码:
A
摘要:
目的：研究埃兹蛋白（Ezrin）的表达对多倍体肿瘤巨细胞（PGCCs）及其子代细胞（PDCs）侵袭、迁移的影响。方法：利用高浓度氯化钴（CoCl2）诱导MDA-MB-231和HEY细胞中PGCCs的形成，将细胞分为对照组（未经CoCl2处理）及PDCs组（经CoCl2处理），通过蛋白免疫印迹实验检测两组Ezrin和上皮-间充质转化（EMT）相关蛋白的表达。通过免疫荧光实验明确Ezrin在PDCs组中的亚细胞定位。利用小干扰RNA瞬时转染技术敲低Ezrin表达，将细胞分为3组：对照组、PDCs组和敲低Ezrin的PDCs组。利用Transwell侵袭实验观察Ezrin 对PDCs侵袭能力的影响。利用Transwell迁移实验和划痕实验探究Ezrin对PDCs迁移能力的影响。结果：蛋白免疫印迹结果显示，与对照组相比，PDCs组EMT相关蛋白E-钙黏蛋白（E-cadherin）表达降低（t=7.662、6.780，均P<0.05），而N-钙黏蛋白（N-cadherin）和波形蛋白（Vimentin）表达升高（t=9.822、9.274、15.64、9.834，均P<0.05），同时EMT相关转录因子Snail和Twist表达也升高（t=6.534、10.80，7.394、12.86，均P<0.05）。免疫荧光结果显示，Ezrin在PDCs胞核中的表达显著增加。Transwell侵袭实验显示，敲低Ezrin表达后PDCs的侵袭能力降低（t=9.101、5.992，均P<0.05），且Transwell迁移实验和划痕实验均显示，敲低Ezrin表达后PDCs的迁移能力降低（t=16.10、5.990、4.443、5.139，均P<0.05）。结论：Ezrin的表达能够影响PDCs的侵袭、迁移能力。
Abstract:
Objective： To investigate the effects of Ezrin expression on the invasion and migration of polyploid giant cancer cells （PGCCs） and their daughter cells （PDCs）. Methods：PGCCs formation was induced in MDA-MB-231 and HEY cells using high-concentration cobalt chloride（CoCl2）. The cells were divided into a control group （untreated with CoCl2） and a PDCs group （treated with CoCl2）. Protein expression of Ezrin and epithelial mesenchymal transition （EMT）-related proteins was assessed via Western blotting analysis in the two groups. Immunofluorescence staining was used to clarify the subcellular localization of Ezrin in the PDCs group. Small interfering RNA （siRNA）-mediated transient transfection was employed to knockdown Ezrin. Cells were divided into three groups： a control group， a PDCs group， and an Ezrin-knockdown PDCs group. Transwell invasion assays were used to investigate the influence of Ezrin on PDCs′ invasive capacity. The effects of Ezrin on the migratory capacity of PDCs were examined using Transwell migration assays and wound-healing assays. Results： Western blotting results showed that， compared with the control group， the PDCs group exhibited reduced expression of the EMT-associated protein E-cadherin （t=7.662， 6.780， both P<0.05）， while the expression of N-cadherin and vimentin was elevated （t=9.822， 9.274， 15.64， 9.834， all P<0.05）. Concurrently， EMT-associated transcription factors Snail and Twist also exhibited elevated expression （t=6.534， 10.80， 7.394， 12.86， all P<0.05）. Immunofluore-scence staining revealed significantly increased nuclear expression of Ezrin in PDCs. Transwell invasion assays demonstrated reduced the invasive capacity of PDCs following Ezrin knockdown（t=9.101， 5.992， both P<0.05）. Transwell migration assays and wound-healing assays indicated that knocking down Ezrin expression suppressed the migratory ability of PDCs （t=16.10， 5.990， 4.443， 5.139， all P<0.05）. Conclusions： The expression of Ezrin can influence the invasive and migratory capabilities of PDCs.

参考文献/References:

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备注/Memo

备注/Memo:
（2025-12-30收稿）
基金项目 天津市卫健委重点项目（TJWJ2025ZK008）
作者简介 梁静静（1999-），女，硕士在读，研究方向：病理学；通信作者：张诗武，E-mail： zhangshiwu666@aliyun.com。
更新日期/Last Update: 2026-07-15