|本期目录/Table of Contents|

[1]张子豪,王艺茹,吴佳佳,等.基于非靶向代谢组学探讨五苓散抗痛风作用机制[J].天津医科大学学报,2025,31(03):262-267.[doi:10.20135/j.issn.1006-8147.2025.03.0262]
　ZHANG Zihao,WANG Yiru,WU Jiajia,et al.Investigation of the anti-gout mechanism of WulingSan based on untargeted metabolomics[J].Journal of Tianjin Medical University,2025,31(03):262-267.[doi:10.20135/j.issn.1006-8147.2025.03.0262]
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基于非靶向代谢组学探讨五苓散抗痛风作用机制(PDF)

《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
31卷
期数:
2025年03期
页码:
262-267
栏目:
基础医学
出版日期:
2025-05-20

文章信息/Info

Title:
Investigation of the anti-gout mechanism of WulingSan based on untargeted metabolomics
文章编号:
1006-8147(2025)03-0262-06
作者:
张子豪; 王艺茹; 吴佳佳; 宋佳音; 吴晓辉
（天津医科大学药学院临床药学教研室，天津300070）
Author(s):
ZHANG Zihao; WANG Yiru; WU Jiajia; SONG Jiayin; WU Xiaohui
（Department of Clinical Pharmacy，School of Pharmacy，Tianjin Medical University，Tianjin 300070，China）
关键词:
痛风; 中药; 五苓散; 代谢组学; 炎症
Keywords:
gout; traditional Chinese medicine; WulingSan; metabolomics; inflammation
分类号:
R9
DOI:
10.20135/j.issn.1006-8147.2025.03.0262
文献标志码:
A
摘要:
目的：研究五苓散（WLS）在痛风大鼠中的作用机制。方法：将30只大鼠随机分为对比组、模型组、西药组、WLS低剂量组（wls_l）和高剂量组（wls_h），每组6只。wls_l和wls_h组分别给予WLS提取物0.06和0.12 g/kg，西药组给予0.1 mg/kg秋水仙碱，对比组和模型组给予生理盐水。每天灌胃两次，持续6 d。第3天，对比组踝关节注射生理盐水，其余组注射尿酸钠晶体以建立痛风模型。实验结束后，检测血清中的炎症因子水平判断药效学结果，并使用液相色谱-质谱/质谱联用进行血清代谢组学分析。根据主成分分析、偏最小二乘法判别分析及正交偏最小二乘判别结果筛选出差异性代谢物并进行富集分析。结果：与模型组相比，各治疗组炎症因子水平均明显降低（P< 0.05或P< 0.01）。代谢组学结果显示痛风大鼠经WLS治疗后，回调了27种代谢物，涉及11条代谢通路，其中最重要的4条代谢通路是花生四烯酸代谢、氨基赖氨酸降解、甘氨酸/丝氨酸/苏氨酸代谢、酪氨酸代谢通路。结论：WLS通过多靶点多通路发挥抗痛风作用。
Abstract:
Objective： To investigate the mechanism of action of WulingSan （WLS） in gouty rats. Methods：Thirty rats were randomly divided into five groups：control，model，Western medicine，low-dose WLS （wls_l），and high-dose WLS （wls_h），with six rats per group. The wls_l and wls_h groups were administered WLS extract at doses of 0.06 g/kg and 0.12 g/kg，respectively，while the Western medicine group received 0.1 mg/kg colchicine. The control and model groups received normal saline. All groups underwent intragastric administration twice daily for six days. On the 3rd day，the control group received an intra-articular injection of normal saline，while the other groups were injected with monosodium urate crystals to establish a gout model. At the end of the experiment，serum inflammatory factor levels were measured to evaluate pharmacological dynamic effects，and serum metabolomics analysis was performed using liquid chro-matography-tandem mass spectrometry （LC-MS/MS）. Differential metabolites were identified through principal component analysis （PCA），partial least squares discriminant analysis （PLS-DA），and orthogonal PLS-DA，followed by enrichment analysis. Results：Compared with the model group，all treatment groups exhibited a significant reduction in inflammatory factor levels （P<0.05 or P<0.01）. Metabolomics analysis revealed that WLS treatment for gout rats restored 27 metabolites associated with 11 metabolic pathways，with the most critical 4 metabolic pathways being arachidonic acid metabolism，amino lysine degradation，glycine/serine/threonine metabolism，and tyrosine metabolism. Conclusion：WLS exerts anti-gout effects through multi-target and multi-pathway mechanisms.

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备注/Memo

备注/Memo:
作者简介: 张子豪（1996-），男，硕士在读，研究方向：临床药理学；
通信作者：吴晓辉，E-mail：xiaohuiwu@tmu.edu.cn。
更新日期/Last Update: 2025-06-01