|本期目录/Table of Contents|

[1]郑燕,高伟,孟庆红,等.自体脂肪间充质干细胞对大鼠肝切除术后早期肝再生的促进作用[J].天津医科大学学报,2024,30(02):152-156.[doi:10.20135/j.issn.1006-8147.2024.02.0152]
　ZHENG Yan,GAO Wei,MENG Qinghong,et al.The promotion of autologous adipose derived mesenchymal stem cells on the early liver regeneration following partial hepatectomy in rats[J].Journal of Tianjin Medical University,2024,30(02):152-156.[doi:10.20135/j.issn.1006-8147.2024.02.0152]
点击复制

自体脂肪间充质干细胞对大鼠肝切除术后早期肝再生的促进作用(PDF)
分享到：

《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
30卷
期数:
2024年02期
页码:
152-156
栏目:
基础医学
出版日期:
2024-03-20

文章信息/Info

Title:
The promotion of autologous adipose derived mesenchymal stem cells on the early liver regeneration following partial hepatectomy in rats
文章编号:
1006-8147（2024）02-0152-05
作者:
郑燕1; 高伟2; 孟庆红3; 龙艺尹1; 韩洪起4; 王玉亮1
（1.天津医科大学第二医院检验科，天津市泌尿外科研究所，天津 300211；2.天津市第一中心医院器官移植中心 ，天津 300192；3.天津市第五中心医院生态城医院检验科，天津 300467；4.优赛生命科学发展有限公司 ，天津 300000）
Author(s):
ZHENG Yan1; GAO Wei2; MENG Qinghong3; LONG Yiyin1; HAN Hongqi4; WANG Yuliang1
（1.Clinical Laboratory，The Second Hospital of Tianjin Medical University，Tianjin Institute of Urology，Tianjin 300121，China； 2.Department of Organ Transplantation Center，Tianjin First Central Hospital，Tianjin 300192，China； 3.Department of Clinical Laboratory Medicine，Eco-City Hospital of Tianjin Fifth Central Hospital，Tianjin 300467，China； 4.Unicell Life Science Development Co.，Ltd，Tianjin 300000，China）
关键词:
间充质干细胞; 脂肪组织; 大鼠; 肝再生
Keywords:
hepatectomy; adipose tissue; mesenchymal stem cell; liver regeneration
分类号:
R392
DOI:
10.20135/j.issn.1006-8147.2024.02.0152
文献标志码:
A
摘要:
目的：观察自体脂肪间充质干细胞（ADSCs）对大鼠70 %肝部分切除术（PH）后早期残肝再生的促进作用。方法：健康雄性Wistar大鼠24只随机分为两组，大鼠70 % PH组（模型组，n=12）、大鼠70% PH后自体ADSCs移植组（移植组，n=12）。比较模型组和移植组术后24、72 h血清谷氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST），获取肝组织，比较形态学改变及增殖细胞核抗原（PCNA）水平，RT-PCR检测肝细胞生长因子（HGF） mRNA表达水平。结果：与模型组比较，移植组术后24 h血清ALT水平显著降低，差异有统计学意义（t=2.925，P<0.05）；与模型组比较，移植组术后24 、72 h血清AST水平显著降低，差异有统计学意义（t=4.139、3.323，均P<0.01）。光镜下观察移植组术后24、72 h肝窦轻度扩张，肝细胞结构排列紊乱程度较模型组轻。与模型组比较，移植组术后24、72 h PCNA指数显著增高，差异有统计学意义（t=3.001、2.825，均P<0.05）。与模型组比较，移植组术后24、72 h HGF mRNA表达水平显著升高，差异有统计学意义（t=2.291、3.263，均P<0.05）。结论：自体ADSCs移植可促进70 % PH后早期残余肝脏再生能力。
Abstract:
Objective：To observe the effect of autologous adipose derived mesenchymal stem cells（ADSCs） on the early liver regeneration after 70% partial hepatectomy（PH） in rats. Methods：Twenty-four healthy male Wistar rats were randomly divided into two groups：rat 70% PH（model group，n=12） and autologous ADSCs transplantation following rat 70% PH group（transplant group，n=12）. Serum glutamic aminotransferase（ALT） and aspartate aminotransferase（AST） were compared between model group and transplant group at 24 and 72 h after operation. The morphological changes and proliferating cell nuclear antigen（PCNA） levels of liver tissues were compared. The mRNA expression levels of hepatocyte growth factor（HGF） were measured by RT-PCR. Results：Compared with model group，the serum ALT level in transplant group at 24 h after operation was significantly decreased，and the difference was statistically significant（t=2.925，P<0.05）. Compared with the model group，the serum AST level in the transplant group was significantly decreased at 24 and 72 h after operation，and the difference was statistically significant（t=4.139，3.323，both P<0.01）. Under light microscope，the hepatic sinuses in transplant group at 24 and 72 h after operation were slightly dilated，and the disorder of hepatocyte structure was significantly reduced than that in model group. Compared with the model group，PCNA index in the transplant group was significantly increased 24 and 72 h after operation，and the difference was statistically significant（t=3.001，2.825，both P<0.05）. Compared with the model group，the mRNA expression level of HGF in the transplantation group was significantly increased at 24 and 72 h after operation，and the difference was statistically significant（t=2.291，3.263，both P<0.01）. Conclusion：Autologous ADSCs transplantation can promote early residual liver regeneration after 70% PH.

参考文献/References:

[1] KIMURA M，MOTEKI H，OGIHARA M. Role of hepatocyte growth regulators in liver regeneration[J]. Cells，2023，12（2）：208.
[2] YAGI S，HIRATA M，MIYACHI Y，et al. Liver regeneration after hepatectomy and partial liver transplantation[J]. Int J Mol Sci，2020， 21（21）：8414.
[3] QIN Y，GE G，YANG P，et al. An update on adipose-derived stem cells for regenerative medicine：where challenge meets opportunity[J]. Adv Sci（Weinh），2023，10（20）：e2207334.
[4] NEVENS F，VAN DER MERWE S. Mesenchymal stem cell transplantation in liver diseases[J]. Semin Liver Dis，2022，42（3）：283-292.
[5] SAITO S，TOGO S，MORIOKA D，et al. A rat model of a repeat 70% major hepatectomy[J]. J Surg Res，2006，134（2）：322-326.
[6] 师帅南，李光，莫春柏，等. 自体脂肪间充质干细胞对大鼠肾冷缺血再灌注损伤的保护作用[J].中华实验外科杂志，2018，35（9）：1616-1618.
[7] SATILMIS B，CICEK G S，CICEK E，et al. Adipose-derived stem cells in the treatment of hepatobiliary diseases and sepsis[J]. World J Clin Cases，2022，10（14）：4348-4356.
[8] 杜鹏，王峰，陈晓波，等. 年龄对人脂肪来源间充质干细胞生物学特性的影响[J]. 天津医药，2021，49（10）：1009-1013.
[9] JIN Y P，SHI R F，QI T K，et al. Adipose-derived stem cells show hepatic differentiation potential and therapeutic effect in rats with acute liver failure[J]. Acta Biochim Biophys Sin（Shanghai），2023，55（4）：601-612.
[10] LALA V，ZUBAIR M，MINTER D A. Liver function tests[M]. In：StatPearls [Internet]. Treasure Island（FL）：StatPearls Publishing，2023：https：//www.ncbi.nlm.nih.gov/books/NBK482489/
[11] GONZ?魣LEZ-MAGA?譙A A，BLANCO F J. Human PCNA structure，function and interactions[J]. Biomolecules，2020，10（4）：570.
[12] PATEL A，ASLAM R，JAMIL M，et al. The effects of growth factors and cytokines on hepatic regeneration：a systematic review[J]. Cureus，2022，14（4）：e24539.
[13] MENG H F，JIN J D，WANG H，et al. Recent advances in the the-rapeutic efficacy of hepatocyte growth factor gene-modified mesenchymal stem cells in multiple disease settings[J]. J Cell Mol Med，2022，26（18）：4745-4755.
[14] WANG P，CUI Y，WANG J，et al. Mesenchymal stem cells protect against acetaminophen hepatotoxicity by secreting regenerative cytokine hepatocyte growth factor[J]. Stem Cell Res Ther，2022，13（1）：94.
[15] CHOI J S，RYU H A，CHEON S H，et al. Human adipose derived stem cells exhibit enhanced liver regeneration in acute liver injury by controlled releasing hepatocyte growth factor[J]. Cell Physiol Bio-chem，2019，52（4）：935-950.
[16] HU C X，WU Z W，LI L J. Mesenchymal stromal cells promote liver regeneration through regulation of immune cells[J]. Int J Biol Sci，2020，16（5）：893-903.

相似文献/References:

[1]袁 晴,杜 雪,袁碧波,等.贴壁法和密度梯度离心法分离经血源性子宫内膜间充质干细胞的比较研究[J].天津医科大学学报,2015,21(06):525.
　YUAN Qing,DU Xue,YUAN Bi-bo,et al. ?Comparison of adherence culture method and density gradient centrifugation method in isolating endometrical mensenchymal stem cells isolated from menstrual blood[J].Journal of Tianjin Medical University,2015,21(02):525.
[2]索睿,郝天旭,茹仙古丽·吾买尔 综述,等.间充质干细胞来源的外泌体治疗哮喘的研究进展[J].天津医科大学学报,2023,29(06):673.
[3]王彪,陈国强,赵清乾,等.N钙黏蛋白融合蛋白改性表面增强细胞活性与细胞募集能力的研究[J].天津医科大学学报,2024,30(06):509.[doi:10.20135/j.issn.1006-8147.2024.06.0509]
　WANG Biao,CHEN Guoqiang,ZHAO Qingqian,et al.Study on surface modification with N-cadherin fusion protein to enhance cell activity and cell recruitment capability[J].Journal of Tianjin Medical University,2024,30(02):509.[doi:10.20135/j.issn.1006-8147.2024.06.0509]

备注/Memo

备注/Memo:
基金项目 国家自然科学基金资助项目（81470982）
作者简介 郑燕（1999-），女，硕士在读，研究方向：医学技术；通信作者：王玉亮，E-mail：wang_yu_l@163.com。
更新日期/Last Update: 2024-03-20