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[1]王芮琦,张云龙,方彦雯,等.静磁场通过促进骨重建和血管生成治疗股骨头坏死[J].天津医科大学学报,2024,30(01):29-34.[doi:10.20135/j.issn.1006-8147.2024.01.0029]
　WANG Ruiqi,ZHANG Yunlong,FANG Yanwen,et al.A static magnetic field protects against osteonecrosis of the femoral head by promoting bone remodeling and angiogenesis[J].Journal of Tianjin Medical University,2024,30(01):29-34.[doi:10.20135/j.issn.1006-8147.2024.01.0029]
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静磁场通过促进骨重建和血管生成治疗股骨头坏死(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
30卷
期数:
2024年01期
页码:
29-34
栏目:
基础医学
出版日期:
2024-01-01

文章信息/Info

Title:
A static magnetic field protects against osteonecrosis of the femoral head by promoting bone remodeling and angiogenesis
文章编号:
1006-8147(2024)01-0029-06
作者:
王芮琦1; 张云龙2; 方彦雯3; 李心乐1; 4; 卫敏3; 廖钟财3; 张平1; 4; 5
（1.天津医科大学基础医学院人体解剖与组织胚胎学系，天津 300070；2.天津医科大学口腔医院口腔综合科，天津 300070；3. 和也健康科技有限公司，安吉 313399；4.国家卫生健康委员会激素与发育重点实验室，天津市代谢性疾病重点实验室，朱宪彝纪念医院，天津医科大学，天津 300134；5.天津市脊柱脊髓重点实验室，天津 300052）
Author(s):
WANG Ruiqi1; ZHANG Yunlong2; FANG Yanwen3; LI Xinle1; 2; WEI Min3; LIAO Zhongcai3; ZHANG Ping1; 4; 5
关键词:
静磁场; 股骨头坏死; 骨形成; 骨吸收; 血管生成
Keywords:
static magnetic field; osteonecrosis of the femoral head; bone formation; bone absorption; angiogenesis
分类号:
R681.8
DOI:
10.20135/j.issn.1006-8147.2024.01.0029
文献标志码:
A
摘要:
目的：探究静磁场对股骨头坏死大鼠骨重建和血管生成的影响。方法：45只雄性Sprague-Dawley（SD）大鼠按照随机数字表法分为假手术组（Sham组，n=15）、股骨头坏死组（ON组，n=15）和股骨头坏死静磁场治疗组（ONS组，n=15）。手术切断大鼠股骨头圆韧带、结扎股骨颈，制造股骨头缺血性坏死模型，使用本课题组研发的200 mT静磁场装置对大鼠进行为期4周的治疗。骨密度仪检测大鼠ON组股骨头组织的骨密度（BMD）和骨矿含量（BMC）。HE染色、MacNeal′s染色、抗酒石酸酸性磷酸酶（TRAP）染色评估组织学改变。Western印迹方法检测Runt相关转录因子2（RUNX2）和活化T-细胞核因子1（NFATc1）重组蛋白的表达水平。墨汁灌注实验分析股骨头血管生成情况。结果：与 ON 组相比， ONS 组大鼠股骨头 BMD 与 BMC 均增加（t=-5.372 、 -4.584，均P<0.001 ）。静磁场抑制了ON组大鼠骨量减少（t=-4.038， P<0.001）、成骨细胞数/骨表面下降（t=-8.227， P<0.001）、破骨细胞数/骨表面增加（t=4.116， P<0.05）和血管生成减少（t=-3.613， P<0.01）。此外，静磁场使RUNX2表达增加（t=-5.850， P<0.01），NFATc1表达降低（t=3.618，P<0.05）。结论：静磁场通过改善骨重建和血管生成，达到治疗股骨头坏死的目的。
Abstract:
Objective： To investigate the role of the static magnetic field on promoting the bone remodeling and angiogenesis in rats with osteonecrosis （ON） of the femoral head. Methods： Forty-five male Sprague-Dawley（SD） rats were divided into three groups by random number table method： the sham control group（Sham group，n=15）， the osteonecrosis group（ON group， n=15）， and the static magnetic field treated osteonecrosis group（ONS group， n=15）. A model of ischemic necrosis of the femoral head was established by surgically cutting the round ligament of the femoral head and ligating the femoral neck，and the 200 mT static magnetic field equipment which de-signed by our research group was used to treat the rats for four weeks. Bone mineral density（BMD） and bone mineral content（BMC） of the femoral head tissue was evaluated by X-ray absorptiometry. HE staining，MacNeal′s staining and anti-alcoholic acidic phosphate chromatin（TRAP） staining were used to examine the histological changes. Western blotting was used to detect the expression of Runt-related transcription factor 2（RUNX2） and the nuclear factor of activated T cells 1 （NFATc1）. Ink perfusion was used to analyze the angiogenesis of femoral head. Results： Compared with ON group， the BMD and BMC of the femoral head in the ONS group were increased（t= -5.372， -4.584， both P<0.001）. Static magnetic field ameliorated the reduction of alleviated the decrease of the bone mass（t= -4.038， P<0.001）， the reduction of osteoblast number/bone surface（t=-8.227， P<0.001）， the increase of osteoclast number/bone surface（t=4.116， P<0.05） and the reduction of bone angiogenesis in the ON group（t=-3.613， P<0.01）. In addition， the static magnetic field promoted the expression of RUNX2（t=-5.850， P<0.01） and reduced the expression of NFATc1（t=3.618， P<0.05）. Conclusion： Static magnetic field can treat osteonecrosis of the femoral head by promoting the bone remodeling and angiogenesis.

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相似文献/References:

备注/Memo

备注/Memo:
基金项目 国家自然科学基金（81772405，81572100）；和也健康科技重明鸟计划（HYCMP-2022016）
作者简介 王芮琦（1997-），女，硕士在读，研究方向：股骨头坏死治疗的机制研究；张云龙（1990-），男，医师，硕士，研究方向：股骨头坏死治疗的机制研究；通信作者：张平， E-mail：pizhang2008@163.com。
更新日期/Last Update: 2024-01-01