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[1]钟天佑,南阳,王乐.SOX5在胶质瘤组织中的表达及对胶质瘤细胞株U87生物学行为的影响[J].天津医科大学学报,2023,29(04):387-390,397.
　ZHONG Tian-you,NAN Yang,WANG Le.Expression of SOX5 in glioma tissue and its effect on biological behavior of glioma cell line U87[J].Journal of Tianjin Medical University,2023,29(04):387-390,397.
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SOX5在胶质瘤组织中的表达及对胶质瘤细胞株U87生物学行为的影响(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
29卷
期数:
2023年04期
页码:
387-390,397
栏目:
基础医学
出版日期:
2023-07-10

文章信息/Info

Title:
Expression of SOX5 in glioma tissue and its effect on biological behavior of glioma cell line U87
文章编号:
1006-8147（2023）04-0387-05
作者:
钟天佑1; 南阳2; 王乐2
1.天津医科大学总医院图书馆，天津300052;2.天津医科大学总医院神经外科，天津300052
Author(s):
ZHONG Tian-you1; NAN Yang2; WANG Le2
1.Department of Library，General Hospital，Tianjin Medical University，Tianjin 300052，China;2.Department of Neurosurgery，General Hospital，Tianjin Medical University，Tianjin 300052，China
关键词:
SOX5基因; U87细胞; 增殖; 侵袭; MMP-2; MMP-9; CyclinD1
Keywords:
SOX5; U87 cells; proliferation; invasion; MMP-2; MMP-9; CyclinD1
分类号:
R826.2
DOI:
-
文献标志码:
A
摘要:
目的：探究SBY-BOX转录因子5（SOX5）基因在胶质瘤组织中的表达及对胶质瘤细胞株U87增殖活性和侵袭能力的影响。方法：胶质瘤和正常脑组织标本各取10例，采用实时定量荧光PCR（RT-PCR）、蛋白质免疫印迹法（Westernblotting）检测SOX5基因的表达，培养胶质瘤细胞株U87，将其分为对照组、无义序列组和siRNASOX5转染组。利用CCK8实验和Transell实验检测胶质瘤细胞株U87增殖活性及侵袭能力。利用Western印迹检测敲低SOX5表达后对基质金属蛋白酶（MMP-2）、MMP-9以及细胞周期蛋白D1（CyclinD1）表达的影响。结果：与正常脑组织相比，胶质瘤组织中SOX5蛋白和mRNA水平均明显升高（t=23.38、18.36，均P＜0.01）。siRNASOX5可有效敲低SOX5基因的表达，与对照组、无义序列组比较，siRNASOX5转染组SOX5蛋白和mRNA水平均明显降低（F=89.31、123.34，均P＜0.01）；CCK8实验结果显示，敲低SOX5表达后，胶质瘤细胞的增殖活性受抑制，第120小时胶质瘤细胞增殖活性仅为对照组的31.8%（F=553.62，P＜0.05）；Traswell实验结果显示，siRNASOX5转染组穿过小室膜细胞数为9.22±0.91，与对照组（19.62±1.10）、无义序列组（18.56±1.21）相比明显降低，仅为对照组的46%（F=89.36，P＜0.01）。与对照组和无义序列组相比，siRNASOX5转染组MMP-2、MMP-9以及CyclinD1基因表达均明显下降（F=853.21、602.30、311.46，均P＜0.01）。结论：SOX5在胶质瘤组织中表达异常升高，敲低SOX5表达后能明显抑制胶质瘤细胞的增殖活性和侵袭能力，SOX5在胶质瘤中可能通过调控MMP-2、MMP-9以及CyclinD1基因表达发挥促癌基因的作用。
Abstract:
Objective：To explore the expression of SOX5（ SRY-box transcription factor 5） gene in glioma tissues and its effect on the proliferation activity and invasion of glioma cell line U87. Methods：Ten cases of glioma tissues and 10 cases of normal brain tissues were collected. The expression of SOX5 gene was detected by real-time quantitative fluorescent PCR （ RT PCR） and Western blotting. Glioma cell line U87 was cultured and divided into control group，nonsense sequence group and siRNA SOX5 transfection group （ n=3 for each group），CCK8 and transwell assay was used to detect the proliferation activity and invasion ability of glioma cell line U87. Western blotting was used to detect the effect of SOX5 knockdown on the expression of matrix metalloproteinase 2 （ MMP-2），matrix metalloproteinase 9（ MMP-9） and G1/S-specific cyclin-D1 （ CyclinD1）. Results：Compared with normal brain tissue，the expression of SOX5 in glioma tissue was significantly higher in protein and mRNA levels （ t=23.38 and 18.36，respectively，both P<0.01）. The siRNA SOX5 could effectively decrease the expression of SOX5 gene. Compared with the control group and the nonsense sequence group，the expression of SOX5 in the siRNA SOX5 transfection group was significantly reduced at the protein and mRNA levels（ F=89.31 and 123.34， respectively，both P<0.01）.The results of CCK8 experiment showed that the proliferation of glioma cells was inhibited after knocking down the expression of SOX5，and the proliferation of glioma cells at 120th hour was only 31.8% of the control group （ F=553.62，P＜0.05）. Transwell experiment results showed that the number of cells passing through the ventricular membrane in the siRNA SOX5 transfection group was（ 9.22 ± 0.91），which was significantly lower than that in the control group （ 19.62±1.10） and the nonsense sequence group （ 18.56 ±1.21），only 46% of the control group （ F=89.36，P<0.01）. Compared with control group and nonsense sequence group，the expression of MMP-2，MMP-9 and CyclinD1 were decreased in siRNA SOX5 transfection group（ F=853.21，602.30 and 311.46， respectively，all P<0.01）. Conclusion：The expression of SOX5 is abnormally increased in glioma tissue. Knocking down the expression of SOX5 can significantly inhibit the proliferation activity and invasion of glioma cells. SOX5 may play a role in cancer promoting genes by regulating the expression of MMP-2，MMP-9 and CyclinD1 genes in glioma.

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备注/Memo

备注/Memo:
作者简介钟天佑（1990-），男，实习研究员，学士，研究方向：胶质瘤的发生机制；
通信作者：王乐，E-mail：wangle1256bai@163.com。
更新日期/Last Update: 2023-07-10