|本期目录/Table of Contents|

[1]岳莹莹,穆红,牛文彦.Axin1调节骨骼肌GLUT4蛋白表达的作用研究[J].天津医科大学学报,2023,29(01):36-40.
　YUE Ying-ying,MU Hong,NIU Wen-yan.Study the effect of Axin1 on regulating GLUT4 protein in skeletal muscle[J].Journal of Tianjin Medical University,2023,29(01):36-40.
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Axin1调节骨骼肌GLUT4蛋白表达的作用研究(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
29卷
期数:
2023年01期
页码:
36-40
栏目:
基础医学
出版日期:
2023-01-20

文章信息/Info

Title:
Study the effect of Axin1 on regulating GLUT4 protein in skeletal muscle
文章编号:
1006-8147(2023)01-0036-05
作者:
岳莹莹1; 穆红1; 牛文彦2
（1.天津市第一中心医院检验科，天津300192；2.天津医科大学基础医学院免疫学系，天津300070）
Author(s):
YUE Ying-ying1; MU Hong1; NIU Wen-yan2
（1.Department of Clinical Laboratory，Tianjin First Central Hospital，Tianjin 300192，China; 2.Department of Immunology，School of Basic Medical Sciences，Tianjin Medical University，Tianjin 300070，China）
关键词:
Axin1; TNKS; GLUT4; 骨骼肌; 葡萄糖摄取
Keywords:
Axin1;  TNKS;  GLUT4;  skeletal muscle;  glucose uptake
分类号:
R392.1
DOI:
-
文献标志码:
A
摘要:
目的：探讨体轴发育抑制因子（Axin1）调节骨骼肌葡萄糖转运蛋白4（GLUT4）蛋白表达的作用及其机制。方法：利用RNAi干扰的Axin1腺病毒（Ad-siAxin1）感染C2C12小鼠骨骼肌细胞敲低Axin1，荧光显微镜和MTS实验明确Ad-siAxin1最佳感染浓度和时间。分别用携带绿色荧光蛋白的腺病毒（Ad-GFP）、Ad-siAxin1、载体质粒（vector）和Axin1质粒转染C2C12小鼠骨骼肌细胞敲低或过表达Axin1蛋白，Western印迹检测Axin1、端锚聚合酶蛋白（TNKS）和GLUT4蛋白水平。结果：荧光显微镜和MTS实验结果显示，在C2C12小鼠骨骼肌细胞中Ad-siAxin1作用的最佳浓度和时间分别为160 μL和48 h。Western 印迹结果显示，与Ad-GFP组相比，Ad-siAxin1组中Axin1蛋白降低（t=6.746，P＜0.01）。Ad-siAxin1组TNKS蛋白水平降低（t=4.019，P＜0.05），GLUT4蛋白水平下调（t=3.248，P＜0.05）。与vector组相比，转染Axin1质粒后，Axin1蛋白水平上调（t=4.868，P＜0.01），TNKS 和GLUT4蛋白水平上调（t=4.897、4.789，均P＜0.01）。结论：Axin1可能通过上调TNKS蛋白表达，调节GLUT4蛋白水平。
Abstract:
Objective： To investigate the role and molecular mechanism of Axin formation inhibitor（Axis1） in regulating glucose transporter 4（GLUT4） protein in skeletal muscle cells. Methods：C2C12 mouse skeletal muscle cells were infected with adenovirus expressing siAxin1（Ad-siAxin1） to knock down Axin1. Fluorescence microscopy and MTS experiments were measured to clarify the optimal infection concentration and time of Ad-siAxin1. C2C12 cells were infected with Ad-GFP（adenovirus expressing green fluorescent protein） or Ad-siAxin1 to knock down Axin1，and transfected with vector plasmid or Axin1 plasmid to overexpress Axin1. The expression of Axin1，Tankyrase（TNKS） and GLUT4 proteins were detected by Western blotting. Results：The results of fluorescence microscopy and MTS experiments showed that the optimal concentration and time of Ad-siAxin1 in C2C12 skeletal muscle cells were 160 μL and 48 h. Western blotting results showed that compared with the Ad-GFP group，Axin1 protein in Ad-siAxin1 group was decreased（t=6.746，P<0.01）. Ad-siAxin1 significantly decreased TNKS protein（t=4.019，P<0.05），and down-regulated GLUT4 protein（t=3.248，P<0.05）.The expression of Axin1 protein was up-regulated after transfection with Axin1 plasmid（t=4.868，P<0.01）.The protein of TNKS and GLUT4 were significantly up-regulated（t=4.897，4.789，both P<0.01）. Conclusion：Axin1 may regulate the level of GLUT4 protein through TNKS.

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金面上项目（81870547）
作者简介: 岳莹莹（1990-），女，博士，研究方向：糖尿病改善机制研究；通信作者：牛文彦，E-mail：wniu@tmu.edu.cn。
更新日期/Last Update: 2023-02-01