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[1]闫墨,刘帅兵,王楷斌,等.基于生物信息学方法筛选精原细胞瘤进展中的脂代谢关键基因[J].天津医科大学学报,2022,28(06):585-590,597.
　YAN Mo,LIU Shuai-bing,WANG Kai-bin,et al.Identification of key lipid metabolism genes in the progress of seminoma by integrated bioinformatics analysis[J].Journal of Tianjin Medical University,2022,28(06):585-590,597.
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
28卷
期数:
2022年06期
页码:
585-590,597
栏目:
生物信息学专题
出版日期:
2022-11-20

文章信息/Info

Title:
Identification of key lipid metabolism genes in the progress of seminoma by integrated bioinformatics analysis
文章编号:
1006-8147(2022)06-0585-06
作者:
闫墨; 刘帅兵; 王楷斌; 陈铭哲; 杨阔
天津医科大学第二医院泌尿外科，天津市泌尿外科研究所，天津 300211
Author(s):
YAN Mo; LIU Shuai-bing; WANG Kai-bin; CHEN Ming-zhe; YANG Kuo
（Department of Urology，Tianjin Institute of Urology，The Second Hospital，Tianjin Medical University，Tianjin 300211，China）
关键词:
精原细胞瘤; TCGA; 脂质代谢; FABP1; 免疫浸润
Keywords:
seminomas; TCGA; lipometabolism; Fatty Acid Binding Protein 1; immune infiltration
分类号:
R737.21
DOI:
-
文献标志码:
A
摘要:
目的：通过生物信息学方法筛选精原细胞瘤进展中的与脂代谢相关的关键基因，分析脂肪酸结合蛋白1（FABP1）在其中可能发挥的作用机制。方法：于TCGA下载精原细胞瘤测序及临床数据，同GSEA网站下载脂代谢相关基因集共同分析，筛选出Ⅱ期、Ⅲ期和Ⅰ期之间的与脂代谢相关的差异基因（MRGs）及关键基因FABP1，利用免疫组化对其表达进行验证，分析其与MRGs表达的相关性、甲基化水平、免疫浸润程度及对预后的影响。结果：Ⅱ期、Ⅲ期精原细胞瘤存在61个上调、4个下调的MRGs。FABP1与差异显著的MRGs有较强的相关性（P＜0.01），GK2、AWAT2、DGAT2L6、ELOVL3在多个甲基化探针均表现明显的甲基化（r=-0.744~-0.489，均P＜0.001）。FABP1与自然杀伤细胞（NK细胞）、中央记忆型T细胞（Tcm细胞）、辅助性T细胞（Th细胞）均存在显著的相关关系（均P＜0.01）；不同FABP1表达的样本中具有明显的免疫浸润（P＜0.001）及基质成分（P＜0.01）差异。高表达FABP1则能显著降低患者无进展生存期。结论：FABP1作为脂代谢关键基因，可能与其他MRGs通过共表达，改变自身甲基化及组织免疫浸润水平，共同促进精原细胞瘤的进展。
Abstract:
Objective： Using bioinformatics method to screen the key lipid metabolism genes in the progress of seminoma and analyze the potential mechanism of fatty acid binding protein 1（FABP1）. Methods: The sequencing and clinical data of seminoma were downloaded from TCGA，and the gene set related to lipid metabolism downloaded from GSEA was used for analysis. The differential genes related to lipid metabolism（MRGs） and the key gene FABP1 between stage Ⅱ/Ⅲ and stage Ⅰ were screened out，and the expression of FABP1 was verified by immunohistochemistry. Then，the correlation between FABP1 and MRGs co-expression，methylation level，immune infiltration degree and its influence on prognosis were analyzed. Results: A total of 61 differentially expressed genes related to lipometabolism were up-regulated and 4 down-regulated in stage Ⅱ/ Ⅲ seminomas. FABP1 was strongly correlated（P＜0.01） with some MRGs，among which，down-regulated genes GK2，AWAT2，DGAT2L6，and ELOVL3 showed significant（r=-0.744--0.489，all P＜0.001） methylation in multiple methylation probes. In seminoma samples，the FABP1 was correlated with natural killer cells（NK cells），central memory T cells（Tcm cells），and helper T cells（Th cells）（all P<0.01），and different FABP1 expression samples showed significant differences in immune infiltration（P＜0.001） and matrix composition（P＜0.01）. Meanwhile，high expression of FABP1 significantly reduced progression-free survival of patients. Conclusion: As a key gene for lipid metabolism，FABP1 may co-express with other MRGs，change auto methylation and tissue immune infiltration level，and jointly promote the progress of seminoma.

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备注/Memo

备注/Memo:
基金项目 天津市科技计划项目（18ZXDBSY00020）
作者简介 闫墨（1997-），男，硕士在读，研究方向：泌尿系肿瘤；
通信作者：杨阔，E-mail：ykuoster@126.com。
更新日期/Last Update: 2022-11-20