|本期目录/Table of Contents|

[1]吕金莹,胡欣,邓嘉胤.抗菌肽RISE-AP12誖对钛种植体周围炎主要致病菌 和生物膜生长的抑制作用研究[J].天津医科大学学报,2022,28(03):310-313.
　LYU Jin-ying,HU Xin,DENG Jia-yin.Study on the inhibitory effect of antibacterial peptide RISE-AP12誖on the main titanium peri-implantitis pathogens and the growth of biofilm[J].Journal of Tianjin Medical University,2022,28(03):310-313.
点击复制

抗菌肽RISE-AP12誖对钛种植体周围炎主要致病菌 和生物膜生长的抑制作用研究(PDF)
分享到：

《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
28卷
期数:
2022年03期
页码:
310-313
栏目:
临床医学
出版日期:
2022-05-20

文章信息/Info

Title:
Study on the inhibitory effect of antibacterial peptide RISE-AP12誖on the main titanium peri-implantitis pathogens and the growth of biofilm
文章编号:
1006-8147(2022)03-0310-04
作者:
吕金莹; 胡欣; 邓嘉胤
（天津医科大学口腔医学院牙周科，天津300070）
Author(s):
LYU Jin-ying; HU Xin; DENG Jia-yin
（Department of Periodontology，School and Hospital of Stomatology，Tianjin Medical University，Tianjin 300070，China）
关键词:
抗菌肽; RISE-AP12誖; 种植体周围炎; 致病菌; 生物膜
Keywords:
antimicrobial peptides; RISE-AP12誖; peri-implantitis; pathogenic bacteria; biofilm
分类号:
R781.05
DOI:
-
文献标志码:
A
摘要:
目的： 探讨抗菌肽RISE-AP12誖对钛种植体周围炎主要致病菌及其生物膜生长的抑制作用。方法： 调整抗菌肽RISEAP12 誖为不同的浓度，对种植体周围炎主要致病菌具梭核杆菌、血链球菌以及牙龈卟啉单胞菌的菌落进行监测，观察抗菌肽 RISE-AP12誖对主要致病菌的影响，确定最小抑菌浓度（MIC）与最小杀菌浓度（MBC），绘制抗菌肽RISE-AP12誖作用于3 种致 病菌的kill-time 曲线并观察其生物膜的变化。结果：抗菌肽RISE-AP12誖对血链球菌、牙龈卟啉单胞菌、具梭核杆菌的MIC 值 分别为20、10、5 mg/L，MBC 值分别为35、15、20 mg/L。根据kill-time 曲线发现， 在1 MIC 和2 MIC 时均具有较好的杀菌作 用，2 MIC 的抑菌作用显著强于1 MIC 与阳性对照组（具核梭杆菌：t=1.653、1.763，P=0.013、0.008；牙龈卟啉单细胞菌：t=0.984、 1.271，P=0.005、0.007；血链球菌：t=1.548、2.187，P=0.023、0.002）。当抗菌肽RISE-AP12誖浓度为40 mg/L 时，对生物膜的抑制作 用最强，清除率显著高于阳性对照组（具核梭杆菌：t=2.768，P=0.032；牙龈卟啉单细胞菌：t=0.676，P=0.014；血链球菌：t=0.794， P=0.025）。结论：抗菌肽RISE-AP12誖对钛种植体周围炎主要致病菌具梭核杆菌、血链球菌以及牙龈卟啉单胞菌均具有抑制作 用，在一定浓度范围内，可以破坏其生物膜生长。
Abstract:
Objective：To investigate the inhibitory effect of antibacterial peptide RISE-AP12誖on the main pathogens of titanium peri-implant inflammation and the growth of biofilm. Methods: The antimicrobial peptide RISE-AP12誖was adjusted to different concentrations， the colonies of Fusobacterium nucleatum，Streptococcus sanguinis and Porphyromonasgingivalis were monitored，the effect of antimicrobial peptide RISE-AP12誖on the main pathogens was observed，and the minimum inhibitory concentration（MIC）and minimum bactericidal concentration（MBC）were determined. The kill-time curves of antimicrobial peptide RISE-AP12誖on three pathogens were drawn，and the changes of biofilm were observed. Results: The MIC values of antimicrobial peptide RISE-AP12誖against Streptococcus sanguis，Porphyromonasgingivalis and Fusobacterium nucleatum were 20，10 and 5 mg/L，and the MBC values were 35，15 and 20 mg/L， respectively. According to the kill-time curve，it was found that both 1 MIC and 2 MIC concentrations had good bactericidal effect，and the bacteriostatic effect of 2 MIC concentration was significantly stronger than that of 1 MIC concentration and positive control group（Fusobac terium nucleatum：t=1.653，1.763，P=0.013，0.008；Porphyromonasgingivalis：t=0.984，1.271，P=0.005，0.007；Streptococcus sanguis： t =1.548，2.187，P=0.023，0.002）. When the concentration of antimicrobial peptide RISE-AP12誖was 40 mg/L，the inhibitory effect on biofilm was the strongest，and the clearance rate was significantly higher than that in the positive control group（Fusobacterium nuclea tum：t=2.768，P=0.032；Porphyromonasgingivalis: t=0.676，P=0.014；Streptococcus sanguis：t=0.794，P=0.025）. Conclusion: Antimicrobial peptide RISE-AP12誖can inhibit the main pathogenic bacteria of titanium periimplant inflammation，such as Fusobacterium nucleatum， Streptococcus sanguis and Porphyromonasgingivalis. In a certain concentration range，it can destroy the growth of biofilm.

参考文献/References:

[1] FRAGKIOUDAKIS I，TSELEKI G，DOUFEXI A E，et al. Current concepts on the pathogenesis of peri-implantitis: a narrative review[J]. Eur J Dent，2021，15（2）：379-387.
[2] NIE J，ZHANG Q，ZHENG H，et al. Pyrosequencing of the subgingival microbiome in peri -implantitis after non -surgical mechanical debridement therapy[J]. J Periodontal Res，2020，55（2）：238-246.
[3] PRANNO N，CRISTALLI M P，MENGONI F，et al. Comparison of the effects of air -powder abrasion，chemical decontamination，or their combination in open-flap surface decontamination of implants failed for peri-implantitis：an ex vivo study[J]. Clin Oral Investig， 2021，25（5）：2667-2676.
[4] GENG H，YUAN Y，ADAYI A，et al. Engineered chimeric peptides with antimicrobial and titanium-binding functions to inhibit biofilm formation on Ti implants[J]. Mater Sci Eng C Mater Biol Appl，2018， 82：141-154.
[5] GODOY-GALLARDO M，MAS-MORUNO C，YU K，et al. Antibacterial properties of hlf1-11 peptide onto titanium surfaces：a comparison study between silanization and surface initiated polymerization[ J]. Biomacromolecules，2015，16（2）：483-496.
[6] HOYOS-NOGUES M，BROSEL-OLIU S，ABRAMOVA N，et al. Impedimetric antimicrobial peptide-based sensor for the early detection of periodontopathogenic bacteria[J]. Biosens Bioelectron，2016， 86：377-385.
[7] 董颖韬，贾斌. 抗菌肽RISE-AP12 联合中药汤剂对种植体 周围炎龈沟液炎症因子的影响[J]. 临床研究，2017，26（11）：60-62.
[8] 谢丽敏，刘丹丹，宗菲. 不同浓度的抗RISE-AP12 对体外培养 成骨细胞生物活性的影响[J]. 口腔医学，2017，37（2）：115-120.
[9] 费涛，李冀寅，王一宇，等.富含色氨酸抗菌肽对种植体周围炎主要 致病菌生长抑制作用的研究[J]. 中国微生态学杂志，2016，28（2）： 156-158，168.
[10] IOANNIS K，CHANTAL P，ALESSANDRO P，et al.Peri-implant diseases：diagnosis，clinical，histological，microbiological character － istics and treatment strategies. a narrative review [J]. Antibiotics， 2020，9：835.
[11] FRANCESCO A，ANDREI C I，MARCO O，et al. Biofilm formation on dental implant surface treated by implantoplasty：an in situ study[J]. Dent J，2020，8：40.
[12] LIYM A，XIANG Q，ZHANG Q H，et al. Overview on the recent study of antimicrobial peptides：origins，functions，relative mechanisms and application[J]. Peptides，2012，37（2）：207-215．
[13] KRUNAL N，MUKTA K，OWEN A，et al. Adherence of oral streptococci to nanostructured titanium surfaces[J]. Dental，2015，9：11.
[14] KEISUKE N，MIDORI S，TAICH IT，et al. Hydroxyl radicals generated by hydrogen peroxide photolysis recondition biofilm-contaminated titaniumsurfaces for subsequent osteoblastic cell proliferation[J]. Sci Rep，2019，9：4688.
[15] CASEY C，PINGHUI F，J覫RGEN S，et al. Herpesvirus-bacteria synergistic interaction in periodontitis[J]. Periodontal，2000，2020，82： 42-64.
[16] FRANCESCO C，DORINA L，CARLO A B，et al. A new strategy against peri-implantitis：antibacterial internal coating[J]. Int J Mol Sci，2019，20：3897.
[17] VESTB Y L，TORSTEIN G，ROGER S，et al.Bacterial biofilm and its role in the pathogenesis of disease[J]. Antibiotics，2020，9（2）：59.
[18] BELIBASAKIS G N，MANOIL D. Microbial community-driven etiopathogenesis of peri-implantitis[J]. JDentRes，2021，100（1）：21-28.

相似文献/References:

备注/Memo

备注/Memo:
基金项目天津市自然科学基金重点项目（17JCYBJC26300）
作者简介:吕金莹（1989-），女，医师，硕士在读，研究方向：口腔医学；
通信作者:邓嘉胤，E-mail：jdeng@tmu.edu.cn。
更新日期/Last Update: 2022-06-01