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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

28卷

期数:

2022年02期

页码:

195-199

栏目:

技术与方法

出版日期:

2022-03-20

文章信息/Info

Title:

Simultaneous determination of plasma concentrations of posaconazole，fluconazole and voriconazole in human plasma by UPLC-MS/MS

文章编号:

1006-8147(2022)02-0195-05

作者:

张飞雨¹; 张瑞霞²; 高慧儿²; 张弋²

（1.天津医科大学一中心临床学院，天津300192；2.天津市第一中心医院药学部，南开大学医学院，天津300192）

Author(s):

ZHANG Fei-yu¹; ZHANG Rui-xia²; GAO Hui-er²; ZHANG Yi²

（1. First Central Clinical College of Tianjin Medical University，Tianjin 300192，China；2.Department of Pharmacy，Tianjin First Central Hospital，School of Medicine，Nankai University，Tianjin 300192，China）

关键词:

泊沙康唑; 氟康唑; 伏立康唑; 超高效液相色谱-串联质谱法; 药物监测; 血药浓度

Keywords:

posaconazole; fluconazole; voriconazole; UPLC-MS/MS; drug monitoring; plasma concentration

分类号:

R9

DOI:

-

文献标志码:

A

摘要:

目的：建立UPLC-MS/MS法同时测定血浆中3个三唑类抗真菌药物（泊沙康唑、氟康唑、伏立康唑）的浓度。方法：采用WatersBEHC18（2.1 mm×50 mm，1.7 μm）色谱柱，流速0.5 mL/min，流动相为甲醇（含2 mmol/L乙酸铵和0.1%甲酸）和2 mmol/L乙酸铵水溶液（含0.1%甲酸），梯度洗脱，柱温40°C。质谱条件采用电喷雾离子源，正离子多反应监测模式进行扫描分析。结果：泊沙康唑、氟康唑、伏立康唑的专属性良好，线性范围分别为0.083~20.680、0.328~82.075、0.080~20.085 μg/mL，范围内线性关系良好，r均大于0.99。批内、批间精密度（RSD）均小于7.60%，提取回收率和基质效应RSD值均小于14.2%，不同储存条件下稳定性良好，RSD值均小于10.69%。结论：该方法准确度高，重复性好，简便快捷，可同时测定3种血浆中三唑类抗真菌药的浓度，适用于临床对氟康唑、泊沙康唑及伏立康唑的治疗药物监测。

Abstract:

Objective：To establish an UPLC-MS/MS method for the simultaneous determination of three triazole antifungal drugs （posaconazole，fluconazole，voriconazole） in plasma. Methods: The chromatographic separation was performed on BEH C18（2.1 mm × 50 mm，1.7 μm） chromatographic column with methanol（containing 2 mmol/L ammonium acetate and 0.1% formic acid） and 2 mmol/L ammonium acetate Aqueous solution（containing 0.1% formic acid）. The column temperature was 40°C. The flow rate was 0.5 mL/min with gradient elution.The mass spectrometry conditions adopt positive electrospray ionization together with multi-reaction monitoring mode for scanning analysis. Results: The specificity of posaconazole，fluconazole and voriconazole was good. The linear ranges were 0.083- 20.680，0.328-82.075，0.080-20.085 μg/mL，respectively. The linear relationship is good within the range，and r was greater than 0.99. The RSD of intra-batch and inter-batch precision were all less than 7.60%. The RSD of extraction recovery rate and matrix effect were all less than 14.2%.The stability was good under different storage conditions，and the RSD were all less than 10.69%. Conclusion: The method is accurate，repeatableand simple. It can simultaneously determine the concentration of three triazole antifungals in plasma. It is suitable for clinical monitoring of fluconazole，posaconazole and voriconazole.

参考文献/References:

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相似文献/References:

备注/Memo

备注/Memo:

作者简介 张飞雨（1996-）,硕士在读，研究方向：临床药学；通信作者：张弋，E-mail：wing_zh1821@sina.com。

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更新日期/Last Update: 2022-03-20