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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

27卷

期数:

2021年06期

页码:

618-622

栏目:

临床医学

出版日期:

2021-11-15

文章信息/Info

Title:

Added rational antibiotics in umbilical cord preservation fluid

文章编号:

1006-8147（2021）06-0618-05

作者:

史丽¹; 2; 郁春艳¹; 李岩¹; 许现辉³; 杨栋林³; 沈洋洋¹; 邓为民¹

（1.天津医科大学基础医学院免疫学系，国家教育部免疫微环境与疾病重点实验室，天津300070；2.协和干细胞基因工程有限公司，天津300384；3.中国医学科学院血液学研究所血液病医院，天津300020）

Author(s):

SHI Li¹; 2; YU Chun-yan¹; LI Yan¹; XU Xian-hui³; YANG Dong-lin³; SHEN Yang-yang¹; DENG Wei-min¹

（1.Department of Immunology，School of Basic Medical Science，Tianjin Medical University，Key Laboratory of Diseases and Microenvironment of Ministry of Education of China，Tianjin 300070，China；2. Union Stem Cell & Gene Engineering Co.，Ltd.，Tianjin 300384，China；3.Institute of Hematology and Blood Diseases Hospital，Chinese Academy of Medical Sciences & Peking Union Medical College，Tianjin 300020，China）

关键词:

脐带采集污染; 菌群分布; 抗生素; 人脐带间充质干细胞

Keywords:

umbilical cord collection contamination; microbial species distribution; antibiotics; human umbilical cord mesenchymal stem cells

分类号:

R378+R379

DOI:

-

文献标志码:

A

摘要:

目的：了解脐带采集过程中脐带污染菌的种类，为脐带采集液中合理添加抗生素提供实验依据。方法：采用BacT/Alert 3D240培养系统进行微生物培养；采用梅里埃API鉴定系统鉴定菌种；采用ATB药敏系统进行药敏试验；采用油红O染色和茜素红染色，测定人脐带组织间充质干细胞（HUCMSCs）的成脂分化和成骨分化潜能。结果：采集1 562份脐带，微生物培养阳性的386份脐带采集液共检出细菌及真菌438株，其中革兰阴性菌193株（44.1%），主要为大肠埃希氏菌，其对头孢西丁的敏感率为90.3%；革兰阳性菌210株（48.0%），主要为肠球菌，其次是葡萄球菌和链球菌，三者对青霉素的敏感率分别为93.1%、19.7%、92.9%；真菌35株（8.0%），主要为白假丝酵母菌，真菌对两性霉素B的敏感率为100%。进一步的结果显示，与对照组比较，头孢西丁、青霉素、两性霉素B和三者混合使用对HUCMSCs生长、增殖和多向分化均无影响（P>0.05）。结论：脐带采集污染优势菌株依次为大肠埃希菌、粪肠球菌、白假丝酵母菌；脐带采集液中加入头孢西丁、青霉素和两性霉素B可以显著减少细菌和真菌污染。

Abstract:

Objective： To identify the microbial species contaminated during collection of umbilical cord and to provide experimental basis for adding rational antibiotics in umbilical cord preservation fluid. Methods: The microbia were cultivated by BacT/Alert 3D240 culture system，identified by API identification system of bioMerieux，and tested by ATB drug sensitivity system for antibiotic sensitivity. Oil red O staining and alizarin red staining were used respectively to detect the pluripotency of human umbilical cord mesenchymal stem cells（HUCMSCs） to adipogenic and osteogenic differentiation. Results:A total of 1 562 human umbilical cords were collected. There were 438 strains of bacteria and fungi detected from 386 specimens of umbilical cord preservation fluid，which were positive in microbial culture. There were 193 strains of gram-negative bacteria，mainly e.coli. The sensitivity rate of e.coli to cefoxitin was 90.3%. There were 210 strains of gram-positive bacteria detected，mainly enterococcus，followed by staphylococcus and streptococcus.The sensitivity rates of enterococcus，staphylococcus and streptococcus to penicillin were 93.1%，19.7% and 92.9%. And there were 35 strains of fungi，mainly candida alba.The sensitivity rate of fungi to amphotericin B were 100.0%. Besides，it made no difference for either cefoxitin，penicillin and amphotericin B applied separately or combinedly to the growth，proliferation and differentiation of HUCMSCs，compared with control group（P>0.05）. Conclusion: The dominant bacteria during collection of umbilical cord are e.coli，enterococcusand candida alba orderly. Cefoxitin，penicillin and amphtericin B can be added into the umbilical cord preservation fluid to reduce contamination.

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相似文献/References:

[1]杨玲,胡志东,田彬,等.2011-2016年临床常见血培养分离病原菌的菌群分布及耐药性变迁[J].天津医科大学学报,2018,24(03):245.
　YANG Ling,HU Zhi-dong,TIAN Bin,et al.Distribution and antibiotic resistance changes of common pathogenic bacteria separated from clinical blood cultures from 2011 to 2016[J].Journal of Tianjin Medical University,2018,24(06):245.

备注/Memo

备注/Memo:

基金项目 天津市教委科研计划项目（2016YD01） 作者简介 史丽（1978-），女，副研究员，硕士在读，研究方向：干细胞研究；通信作者：邓为民，E-mail:dengweimin@tmu.edu.cn。

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更新日期/Last Update: 2021-11-15