|本期目录/Table of Contents|

[1]孙干成,侯永强,高 杰,等.PHF20相互作用蛋白质组学分析及其在肿瘤中的作用研究[J].天津医科大学学报,2019,25(06):563-566.
　SUN Gan-cheng,HOU Yong-qiang,GAO Jie,et al.A study of proteomic analysis of the PHF20 interactome and its role in tumorigenesis[J].Journal of Tianjin Medical University,2019,25(06):563-566.
点击复制

PHF20相互作用蛋白质组学分析及其在肿瘤中的作用研究(PDF)
分享到：

《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
25卷
期数:
2019年06期
页码:
563-566
栏目:
基础医学
出版日期:
2019-11-20

文章信息/Info

Title:
A study of proteomic analysis of the PHF20 interactome and its role in tumorigenesis
文章编号:
1006-8147(2019)06-0563-04
作者:
孙干成; 侯永强; 高 杰; 邱熔芳
（天津医科大学基础医学院生物化学与分子生物学系，天津300070）
Author(s):
SUN Gan-cheng; HOU Yong-qiang; GAO Jie; QIU Rong-fang
（1.Department of Biochemistry and Molecular Biology，School of Basic Medical Sciences, Tianjin Medical University，Tianjin 300070，China）
关键词:
PHF20; KDM4A; PRC2; 恶性肿瘤
Keywords:
PHF20; KDM4A; PRC2; tumorigenesis
分类号:
Q7+R730
DOI:
-
文献标志码:
A
摘要:
目的：探究PHF20相互作用蛋白，初步揭示PHF20促进肿瘤发生发展的分子机制。方法：通过免疫亲和纯化联合银染质谱的方法检测PHF20的相互作用蛋白。免疫共沉淀方法对质谱的结果进行验证。在干扰PHF20及对照乳腺癌细胞系中，用EdU和转移小室的实验检测PHF20在乳腺癌发生发展中的作用。结果：质谱结果显示，PHF20可以结合KDM4A和PRC2等复合物，免疫共沉淀验证了PHF20与各个复合物之间相互作用的可信性。EdU和转移小室实验表明，干扰PHF20后与对照细胞相比，其增殖和侵袭能力显著降低。结论：PHF20在体内与多种表观遗传调控相关的复合物相互作用，并促进肿瘤细胞增殖和侵袭的能力。
Abstract:
Objective:To investigate the proteins that are associated with PHF20, and to explore the mechanism of PHF20 promoting carcinogenesis. Methods:Affinity purification and mass spectrometry assays were used to identify the proteins that are associated with PHF20 in vivo. Co-IP was performed with antibodies to verify the mass spectrometry results. In the PHF20 knockdown cells and the control cells, we examined the cellular proliferation and invasion of the breast cancer cell lines using an EdU and transwell assays. Results:The results of mass spectrometry indicated that PHF20 co-purified with NuRD（MTA1），KDM4A and PRC2 complex in vivo, and the presence of these proteins in the PHF20-interacting complex was confirmed by coimmunoprecipitation experiments. Compared to control cells, EdU and transwell assays showed that knockdown of PHF20 led to a statistically significant decrease in the proliferation and invasive potential of MDA-MB-231cells. Conclusion:PHF20 interacts with multiple transcriptional regulation complexes in vivo and promotes the proliferation and invasive potential of tumorigenesis.

参考文献/References:

[1] Liu T, Zhang T, Zhou F, et al. Identification of genes and pathways potentially related to PHF20 by gene expression profile analysis of glioblastoma U87 cell line[J]. Cancer Cell Int, 2017, 17: 87
[2] Badeaux A I, Yang Y, Cardenas K, et al. Loss of the methyl lysine effector protein PHF20 impacts the expression of genes regulated by the lysine acetyltransferase MOF[J]. J Biol Chem, 2012, 287(1): 429
[3] Adams-Cioaba M A, Li Z, Tempel W, et al. Crystal structures of the Tudor domains of human PHF20 reveal novel structural variations on the Royal Family of proteins[J]. FEBS Lett, 2012, 586(6): 859
[4] Park S, Kim D, Dan H C, et al. Identification of Akt interaction protein PHF20/TZP that transcriptionally regulates p53[J]. J Biol Chem, 2016, 291(43): 22852
[5] Pallasch C P, Struss A K, Munnia A, et al. Autoantibodies against GLEA2 and PHF3 in glioblastoma: tumor-associated autoantibodies correlated with prolonged survival[J]. Int J Cancer, 2005, 117(3): 456
[6] Zhang T, Park K A, Li Y W, et al. PHF20 regulates NF-kappaB signalling by disrupting recruitment of PP2A to p65[J]. Nat Commun, 2013, 4: 2062
[7] Taniwaki M, Daigo Y, Ishikawa N, et al. Gene expression profiles of small-cell lung cancers: molecular signatures of lung cancer[J]. Int J Oncol, 2006, 29(3): 567
[8] Li Y W, Park J, Piao L Z, et al. PKB-mediated PHF20 phosphorylation on Ser291 is required for p53 function in DNA damage[J]. Cell Signall, 2013, 25(1): 74
[9] Chen W, Sun K, Zheng R, et al. Cancer incidence and mortality in China, 2014[J]. Chin J Cancer Res, 2018, 30(1): 1
[10] Margueron R, Reinberg D. The polycomb complex PRC2 and its mark in life[J]. Nature, 2011, 469(7330): 343
[11] Chase A, Cross N C. Aberrations of EZH2 in cancer[J]. Clin Cancer Res, 2011, 17(9): 2613
[12] Guerra-Calderas L, Gonzalez-Barrios R, Herrera L A, et al. The role of the histone demethylase KDM4A in cancer[J]. Cancer Genet, 2015, 208(5): 215

相似文献/References:

备注/Memo

备注/Memo:
作者简介 孙干成（1993-），男，硕士在读，研究方向：表观遗传与肿瘤；通信作者：邱熔芳，E-mail: rongfangqiu@tmu.edu.cn。
更新日期/Last Update: 2020-01-20