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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

25卷

期数:

2019年05期

页码:

455-458

栏目:

基础医学

出版日期:

2019-09-20

文章信息/Info

Title:

Insulin secretion levels in cells of different parts of mouse small intestine

文章编号:

1006-8147(2019)05-0455-04

作者:

周翰驰; 陈 皓; 张 瑞; 郭 刚

（天津医科大学代谢病医院内分泌研究所，卫生部激素与发育重点实验室，天津 300070）

Author(s):

ZHOU Han-chi;  CHEN Hao;  ZHANG Rui;  GUO Gang

（Institute of Endocrinology，Metabolic Disease Hospital，Tianjin Medical University，Tianjin 300070，China）

关键词:

小肠; 胰岛素; 小鼠; 胰外胰岛素

Keywords:

small intestine;  insulin;  mouse;  extra-pancreatic insulin

分类号:

R587.1

DOI:

-

文献标志码:

A

摘要:

目的：探究小鼠小肠细胞是否能够合成胰岛素以及十二指肠、空肠、回肠分泌胰岛素的水平是否有差异。方法：以健康C57BL/6J小鼠的小肠为研究对象，将小肠分为十二指肠、空肠、回肠3部分，通过电子扫描显微镜、免疫荧光、PCR和Western blot对小肠中的胰岛素进行检测。结果：免疫荧光检测结果显示，在小鼠小肠的肠绒毛及肠隐窝有胰岛素、PDX1及ICA-512的表达。电子扫描显微镜观察发现小肠隐窝附近的细胞内有类似胰岛素的分泌颗粒。RT-PCR和Western blot检测结果显示，小肠中有胰岛素mRNA及蛋白质表达。结论：小肠中确实存在胰岛素表达，其中空肠的胰岛素表达水平最高，十二指肠和回肠的表达水平稍低。

Abstract:

Objective: To investigate whether mouse intestine cells in mice can synthesize insulin and whether the levels of insulin secreted by duodenum, jejunum and ileum are different. Methods: We used the small intestine of healthy C57BL/6J mice as the research object. The small intestine was divided into three parts: duodenum, jejunum and ileum. The insulin was detected by scanning electron microscopy, immunofluorescence, PCR and western blot. Results: Immunofluorescence assay showed that insulin, PDX1 and ICA-512 were expressed in the intestinal villi and intestinal crypts of the small intestine. Electron scanning microscopy revealed that there were insulin-like secretory granules in the cells near the small intestine crypt. RT-PCR and western blot showed that insulin mRNA and protein were expressed in the small intestine. Conclusion: There is indeed insulin expression in the small intestine. Among them, the expression level of insulin in the jejunum is the highest, and the expression levels in the duodenum and ileum are slightly lower.

参考文献/References:

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备注/Memo

备注/Memo:

基金项目 天津市科委重点项目资助（14JCZDJC33700）
作者简介 周翰驰（1992-），男，硕士在读，研究方向：生物化学与分子生物学；通信作者：郭刚，E-mail:guogangtj@126.com。

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更新日期/Last Update: 2019-10-11