|本期目录/Table of Contents|

[1]徐雷倩,曹 洁.FSTL1促进间歇性低氧诱导的肺成纤维细胞活化[J].天津医科大学学报,2019,25(05):446-449+454.
　XU Lei-qian,CAO Jie.FSTL1 promotes intermittent hypoxia-induced lung fibroblast activation[J].Journal of Tianjin Medical University,2019,25(05):446-449+454.
点击复制

FSTL1促进间歇性低氧诱导的肺成纤维细胞活化(PDF)
分享到：

《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
25卷
期数:
2019年05期
页码:
446-449+454
栏目:
基础医学
出版日期:
2019-09-20

文章信息/Info

Title:
FSTL1 promotes intermittent hypoxia-induced lung fibroblast activation
文章编号:
1006-8147(2019)05-0446-04
作者:
徐雷倩; 曹 洁
（天津医科大学总医院呼吸科，天津300052）
Author(s):
XU Lei-qian; CAO Jie
（Department of Respiratory， General Hospital, Tianjin Medical University ，Tianjin 300052，China）
关键词:
睡眠呼吸暂停综合征; 卵泡抑素样蛋白1; 肺纤维化; 间歇低氧; 氧化应激
Keywords:
sleep apnea syndrome;  follistatin-like protein 1;  pulmonary fibrosis;  intermittent hypoxia;  oxidative stress
分类号:
R563
DOI:
-
文献标志码:
A
摘要:
目的：探究FSTL1与模拟睡眠呼吸暂停间歇低氧模式下氧化应激反应诱导肺成纤维细胞活化的关系。方法：将小鼠原代肺成纤维细胞分为1、3、6、8 h间歇低氧组，8 h间歇低氧加抗氧化剂Temple组和常规对照组。提取细胞内蛋白，检测氧化应激指标MDA、CAT。提取细胞内蛋白和培养基上清中蛋白，做Western blot分析。沉默肺成纤维细胞中FSTL1的表达，重复上述低氧处理，再次检测氧化应激指标并做Western blot分析。结果：间歇低氧处理后，氧化应激指标MDA和CAT随时间增加而上升，Temple可抑制氧化应激反应。Western blot显示α-SMA和FSTL1的表达与氧化应激指标趋势一致。沉默FSTL1表达后，氧化应激指标呈下降趋势，Collagen 1和α-SMA表达降低。结论：间歇低氧能够造成氧化应激反应，成纤维细胞的活化，这种影响可能与FSTL1的表达相关。
Abstract:
Objective: To investigate the relationship between FSTL1 and oxidative stress response induced by intermittent sleep hypoapnea in lung fibroblasts. Methods：（1） Mouse lung fibroblasts were divided into 1, 3, 6 and 8 hours intermittent hypoxia group, 8 hours intermittent hypoxia plus antioxidant Temple group and conventional control group. （2） Intracellular proteins and detect oxidative stress indicators MDA, CAT were extracted. （3） The protein in the intracellular protein and the culture supernatant were extracted and western blot analysis was performed. （4） We also did the following：Silencing the expression of FSTL1 in lung fibroblasts, repeating the above hypoxia treatment, re-detecting the oxidative stress index and performing western blot analysis. Results： After intermittent hypoxia treatment, oxidative stress indicators MDA and CAT increased with time, and Temple inhibited oxidative stress. Western blot showed that the expressions of α-SMA and FSTL1 were consistent with the trend of oxidative stress. After silencing FSTL1 expression, the oxidative stress index showed a downward trend, and Collagen 1 and α-SMA expression decreased. Conclusion： Intermittent hypoxia can cause oxidative stress and activation of fibroblasts, which may be related to the expression of FSTL1.

参考文献/References:

[1] Arbeitsgruppe für pdiatrische Schlafmedizin und Schlafforschung der sterreichischen Gesellschaft für Kinder-und Jugendheilkunde（GKJ）.Schnarchen und obstruktives Schlafapnoesyndrom(OSAS)[J]. Mona-tsschrift Kinderheilkunde，2011, 159(7):667
[2] Nural S, Gunay E, Halici B, et al. Inflammatory processes and effects of continuous positive airway pressure (CPAP) in overlap syndrome[J]. Inflammation， 2013,36(1): 66
[3] Yamakawa H, Shiomi T, Sasanabe R, et al. Pulmonary hypertension in patients with severe obstructive sleep apnea[J]. Psych Clin Neurosciences， 2002, 56(3): 311
[4] Mermigkis C, Bouloukaki I, Antoniou K M, et al. CPAP therapy in patients with idiopathic pulmonary fibrosis and obstructive sleep apnea: does it offer a better quality of life and sleep[J]. Sleep Breath Schlaf Atmung，2013,17(4):1137
[5] Dong Y, Geng Y, Li L, et al. Blocking follistatin-like 1 attenuates bleomycin-induced pulmonary fibrosis in mice[J]. J Exper Med，2015, 212(2): 235
[6] Li M, Krishnaveni M S, Li C, et al. Epithelium-specific deletion of TGF-beta receptor type II protects mice from bleomycin-induced pulmonary fibrosis[J]. Clin Invest，2011,121(1): 277
[7] Nair D, Dayyat E A, Zhang S X, et al. Intermittent hypoxia-induced cognitive deficits are mediated by NADPH oxidase activity in a murine model of sleep apnea[J]. PloS One，2011, 6(5): e19847
[8] Passali D, Corallo G, Petti A, et al. A comparative study on oxidative stress role in nasal breathing impairment and obstructive sleep apnoea syndrome[J]. Acta otorhinolaryngologica Italica : organo ufficiale della Societa italiana di otorinolaringologia e chirurgia cervico-facciale，2016,36(6): 490
[9] Franklin K A, Lindberg E. Obstructive sleep apnea is a common disorder in the population - a review on the epidemiology of sleep apnea[J]. J Thorac Dis，2015,7(8):1311
[10] Drager L F, Brunoni A R, Jenner R, et al. Effects of CPAP on body weight in patients with obstructive sleep apnoea: a meta-analysis of randomised trials[J]. Thorax，2015,70(3): 258
[11] Hayakawa S, Ohashi K, Shibata R, et al. Association of Circulating Follistatin-Like 1 Levels with Inflammatory and Oxidative Stress Markers in Healthy Men[J]. PloS One，2016,11(5): e0153619
[12] Sylva M, Li V S, Buffing A A, et al. The BMP antagonist follistatin-like 1 is required for skeletal and lung organogenesis[J]. PloS one，2011,6(8):e22616
[13] Liu X, Liu Y, Yang Z, et al. Cell type specific expression of Follistatin-like 1 (Fstl1) in mouse embryonic lung development[J]. J Mol Histol，2018
[14] Mattiotti A, Prakash S, Barnett P, et al. Follistatin-like 1 in development and human diseases[J]. CMLS，2018,75(13): 2339
[15] Fang Y, Zhang S, Li X, et al. Follistatin like-1 aggravates silica-induced mouse lung injury[J]. Sci Reports，2017,7(1): 399

相似文献/References:

备注/Memo

备注/Memo:
基金项目 国家自然科学基金资助项目（81670084，81600067）
作者简介 徐雷倩（1993-），女，硕士在读，研究方向：睡眠低氧性疾病和肺间质病；通信作者：曹洁，E-mail：tjcaojie@sina.com。
更新日期/Last Update: 2019-10-11