|本期目录/Table of Contents|

[1]张荣格,张瑞霞,张 弋,等.高效液相色谱法测定替考拉宁血药浓度[J].天津医科大学学报,2019,25(03):296-299.
　ZHANG Rong-ge,ZHANG Rui-xia,Zhang Yi,et al.Determination of concentration of teicoplanin in plasma by HPLC[J].Journal of Tianjin Medical University,2019,25(03):296-299.
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
25卷
期数:
2019年03期
页码:
296-299
栏目:
技术与方法
出版日期:
2019-05-20

文章信息/Info

Title:
Determination of concentration of teicoplanin in plasma by HPLC
文章编号:
1006-8147(2019)03-0296-04
作者:
张荣格1; 张瑞霞2; 张 弋2; 张彦文3
（1.天津医科大学药学院药物化学教研室，天津 300070；2天津市第一中心医院药学部，天津 300192；3.天津医学高等专科学校，天津300222）
Author(s):
ZHANG Rong-ge１;  ZHANG Rui-xia2;  Zhang Yi2;  ZHANG Yan-wen3
（1.Department of Pharmaceutical Chemistry, School of Pharmacy，Tianjin Medical University, Tianjin 300070, China; 2.Department of Pharmacy，Tianjin First Center Hospital，Tianjin 300192, China; 3.Tianjin Medical college, Tianjin 300222, china）
关键词:
替考拉宁; 高效液相色谱法; 血药浓度
Keywords:
teicoplanin; HPLC; plasma concentration
分类号:
R9
DOI:
-
文献标志码:
A
摘要:
目的：建立高效液相色谱（HPLC）法检测人血浆中替考拉宁浓度的方法。方法：采用Waters System-C18色谱柱，以乙腈：0.01mol/L磷酸二氢钠缓冲液（25:75，V/V，H3PO4调pH值为2.53）为流动相，柱温40 ℃，流速1.2 mL/min；进样量20 L；检测波长215nm。结果：本方法得到标准曲线方程为y = 0.053x - 0.021（R2= 0.998），人血浆中替考拉宁浓度在3.125～100 mg/L范围内具有良好的线性关系。回收率大于92%，精密度和稳定性的RSD均小于8%。结论：本研究建立的替考拉宁血药浓度HPLC测定法专属性好、操作简单、准确度高、重现性好、回收率和灵敏度高，适用于生物样本分析研究，可满足替考拉宁药动学研究的需要。
Abstract:
Objective: To establish a HPLC method to determine teicoplanin in human plasma. Methods：Chromatographic conditions included Waters C18 column, acetonitrile-0.01 mol/L NaH2PO4（25:75，V/V，pH adjusted to 2.53 with H3PO4）as mobile phase, 40 ℃ column temperature, 215 nm detective wavelength, and 1.2 mL/min flow rate. Results：This method generated a standard curve of teicoplanin with good linearty in the concentration of 3.125～100 mg/L（R2= 0.998）,the recovery was over 92%, and the precision and the stability were also qualified as their RSD were below 8%. Conclusion：We believe this method is sensitive, simple, accurate, precisely, and stable. It could be used for the determination of teicoplanin in plasma and fulfill the needs of clinical pharmacokinetics for teicoplanin.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介 张荣格（1993-），女，硕士在读，研究方向：临床药学研究；通信作者：张弋，E-mail：wing_zh1821@sina.com；张彦文，E-mail：zhangyanwen@tijmu.edu.cn。
更新日期/Last Update: 2019-07-03