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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

25卷

期数:

2019年03期

页码:

205-209224

栏目:

基础医学

出版日期:

2019-05-20

文章信息/Info

Title:

Triptolide affects epithelial-mesenchymal transition of renal tubular epithelial cells by regulating TGF-β1 and Hippo signaling pathways

文章编号:

1006-8147(2019)03-0205-06

作者:

臧 勇1; 田 园1; 2;  李卫东1

（1.天津医科大学基础医学院遗传学系，天津 300070；2. 贵阳市第一人民医院输血科，贵阳 550002）

Author(s):

ZANG Yong1;  TIAN Yuan1; 2;  LI Wei-dong1

（1.Department of Genetics, School of Basic Medical Sciences, Tianjin Medical University,Tianjin 300070, China; 2. Department of Blood Transfusion, The First People’s Hospital of Guiyang, Guiyang 550002, China）

关键词:

雷公藤甲素; 上皮间充质转化; 纤维化; 肾小管上皮细胞

Keywords:

triptolide; epithelial mesenchymal transformation; fibrosis; renal tubular epithelial cells

分类号:

R915

DOI:

-

文献标志码:

A

摘要:

目的：研究雷公藤甲素（TP）对肾小管纤维化抑制作用的机制。方法：将HK-2分成3组，阴性对照组不做处理，TGF-β1组用 TGF-β1处理，TGF-β1+TP组用TGF-β1和TP处理，培养48 h观察细胞的形态，利用RNA 测序技术检测各组中mRNA的表达，通过KEGG基因富集筛选相关通路，用Western blot检测EMT相关蛋白及富集所得信号通路中重要蛋白的表达。结果：TP处理可致HK-2形态改变。RNA测序和KEGG富集分析显示，TP显著影响TGF-β信号通路和Hippo信号通路相关基因的表达。Western blot结果表明TP可影响HK-2细胞EMT相关蛋白、TGF-β1信号通路和Hippo信号通路中的重要指标蛋白的表达。结论：TP可通过调节TGF-β1信号通路和Hippo信号通路抑制肾小管上皮细胞发生上皮-间充质转化。

Abstract:

Objective: To investigate the mechanism of the inhibition of triptolide(TP) on renal tubular fibrosis, by using renal tubular epithelial cells(HK-2) as a model system to identify TP regulated signaling pathways through RNA sequencing and Western blot. Methods: HK-2 cells were divided into 3 groups: the untreated normal control group, the TGF-β1 group(cells were stimulated by 20 ng/mL TGF-β1), and the TGF-β1+TP group（cells were stimulated by 20 ng/mL TGF-β1 and 10 nmol/L TP）. Cells were observed by microscope after cultured for 48 h. RNA sequencing was performed to detected mRNA expressions in different groups. KEGG pathway enrichment was performed to analyze the differential expressed genes. Western blot was used to detected the various proteins of EMT, TGF-β1, and Hippo pathway markers. Results: NK-2 cells morphology changed significantly when stimulated by TP. RNA sequencing and KEGG pathway enrichment analyses showed that TP had impacts on TGF-β and Hippo signaling pathways. Expressions of marker proteins of EMT, TGF-β1, and Hippo signaling pathways, such as E-cadherin, N-cadherin, Vimentin, Smad2/3, TGF-βR1, and TAZ were changed significantly when HK-2 cells were stimulated by TP（P<0.05）. Conclusion: TP may affect the expressions of TGF-β1 and Hippo signaling pathway hallmark genes, and inhibited the occurrence of EMT that induced by TGF-β1.

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相似文献/References:

[1]姚晶瑞,姜埃利,王立华,等.雷公藤甲素上调PRDX2抑制2型糖尿病肾病的氧化应激状态[J].天津医科大学学报,2019,25(05):466.
　YAO Jing-rui,JIANG Ai-li,WANG Li-hua,et al.Inhibition of Triptolide on oxidase stress via PRDX2 expression in diabetic nephropathy[J].Journal of Tianjin Medical University,2019,25(03):466.

备注/Memo

备注/Memo:

基金项目 国家重点研发计划项目资助（2017YFC1001900）
作者简介 臧勇（1992-），男，硕士在读，研究方向：病理与病理生理学；通信作者：李卫东，E-mail:liweidong98@tmu.edu.cn；田园，E-mail:1129424744@ qq.com。

常用功能

更新日期/Last Update: 2019-07-03