|本期目录/Table of Contents|

[1]王 娅,庄布军,吴伯岳,等.Mn-ZnS量子点室温磷光检测尿液中硫酸庆大霉素[J].天津医科大学学报,2018,24(01):68-72.
　WANG Ya,ZHUANG Bu-jun,WU Bo-yue,et al.Determination of gentamycin sulfat in urine by room-temperature phosphorescence sensor based on Mn-ZnS quantum dots[J].Journal of Tianjin Medical University,2018,24(01):68-72.
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
24卷
期数:
2018年01期
页码:
68-72
栏目:
技术与方法
出版日期:
2018-01-20

文章信息/Info

Title:
Determination of gentamycin sulfat in urine by room-temperature phosphorescence sensor based on Mn-ZnS quantum dots
文章编号:
1006-8147(2018)01-0068-05
作者:
(1. 天津医科大学第二医院药学部，天津 300211；2.天津医科大学药理学系，天津 300070；3.天津医科大学药学院，天津 300070）
Author(s):
WANG Ya1; 2; ZHUANG Bu-jun2; WU Bo-yue3; GAO Wei-zhen2; 3

(1.Department of Pharmacy, TheSecond Hospital, Tianjin Medical University,Tianjin 300211,China;2.Department of Pharmacology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China; 3. College of Pharmacy,, Tianjin Medical University, Tianjin 300070, China)

关键词:

量子点; 磷光法检测; 硫酸庆大霉素

Keywords:

quantum dots;  phosphorescencedetection; gentamicin sulfate

分类号:
R927.2
DOI:
-
文献标志码:
A
摘要:

目的：基于硫酸庆大霉素对Mn掺杂ZnS量子点的磷光增敏作用，建立了一种简便且灵敏测定硫酸庆大霉素浓度的新方法。方法：在水相中合成巯基丙酸（MPA）包裹的Mn掺杂ZnS量子点，实验中考察了缓冲溶液pH和类型、反应时间及量子点浓度等因素对室温磷光检测的影响。利用荧光分光光度计通过测定Mn掺杂ZnS量子点磷光强度的变化，实现对尿液中硫酸庆大霉素以及硫酸庆大霉素注射液浓度的测定。结果：成功合成了浓度为2×10-2 mg/L的Mn掺杂ZnS量子点。在pH=7.4的三羟甲基氨基甲烷-盐酸缓冲体系中，静置反应时间为30min条件下，硫酸庆大霉素浓度与Mn掺杂ZnS量子点磷光强度线性关系良好且相关系数R2=0.998 5。结论：该方法灵敏、快捷，可以用于测定尿液中硫酸庆大霉素的浓度。

Abstract:

Objective:Toestablish a simple and sensitive method for the determination of gentamicin sulfate based on the photosensitization of Mn-ZnS quantum dots.Methods:Mercator propionic acid (MPA) modifiedMn-ZnS quantum dots were synthesized in aqueous phase. The effects of pH，type of buffer solution, reaction time and concentration of quantum dots on the room-temperature phosphorescent detection were investigated.The concentrations of gentamicin sulfate and gentamicin sulfate in urine were determined by measuring the phosphorescence intensity of Mn-ZnS quantum dots with a fluorescence spectrophotometer. Results: The Mn-ZnS quantum dots were successfully synthesized. The linear relationship between gentamicin sulfate and phosphorescence intensity of the Mn-ZnS quantum dots (2×10-2 mg/L) was obtained with a correlation of 0.998 5 after 30 min incubation in Tris-HCl buffer (pH=7.4) at room temperature.Conclusion：The proposed method is sensitive and simple and can be used for the determination of gentamicin sulfatein urine.

参考文献/References:

[1] 朱立勤,娄建石,焦建杰, 等. 庆大霉素不同给药方案的药代动力学和肾功能损害的实验研究[J]. 中国药理学通报,2006,22(8):1009

[2] Garneau-Tsodikova S,Labby KJ. Mechanisms of Resistance to Aminoglycoside Antibiotics: Overview and Perspectives[J]. Medchemcomm,2016,7 (1):11

[3] Li J,Woo CW.Finding ways to solve or preventaminoglycoside-inducedototoxicity[J]. Ann Transl Med,2016, 4(24):533

[4]GerlachAT,Stawicki SP,Cook CH,et al. Risk factors for aminoglycoside-associated nephrotoxicity in surgical intensive care unit patients[J]. Int J Crit Illn Inj Sci,2011 ,1(1):17

[5] Touw DJ,WestermanEM,Sprij AJ. Therapeutic drug monitoring of aminoglycosides in neonates[J].ClinPharmacokinet, 2009,48 (2):71

[6]曾萍,杨镒宇,陶建平,等. 危重病患儿口服庆大霉素血药质量浓度监测的意义[J].实用儿科临床杂志,2006,21(6):339

[7] 彭小林,范亚新,张亮,等.氨基糖苷类抗生素治疗药物浓度监测的研究进展[J]. 中国感染与化疗杂志,2017,17(1):104

[8] Mahmoudi L,Mohammadpour AH,Ahmadi A, et al. Influence of sepsis on higher daily dose of amikacin pharmacokinetics in critically ill patients [J]. Eur Rev Med PharmacolSci,2013, 17(3):285

[9] Rodriquez M,Cretoso DS,Euterpio MA,et al. Fast determination of underivatized gentamicin C components and impurities by LC-MS using a porous graphitic carbon stationary phase[J]. Anal BioanalChem, 2015, 407(25):7691

[10] 李玮,胡昌勤,王明娟. 高效液相色谱法测定庆大霉素C组分的不同检测方式测定结果的比较[J]. 色谱, 2007, 25(4):557

[11] 杨利红，常艳，姚尚辰，等. HPLC法同时测定庆大霉素的组分纯度和效价活性[J]. 药学学报，2012,12:105

[12] 潘进,邱凌峰,方圣琼,等. 衍生化紫外分光光度法测定硫酸庆大霉素含量[J]. 环境工程, 2015, 33:295

[13] Tan L,Li Y,Tang Y,et al. Room temperature phosphorescence sensor for Hg2+ based on Mn-doped ZnS quantum dots[J].JNanosciNanotechnol.2012,12(10):7788

[14] Zhao MX,Zeng EZ. Application of functional quantum dot nanoparticles as fluorescence probes in cell labeling and tumor diagnostic imaging[J]. Nanoscale Res Lett, 2015, 10(1):171

[15] Wu P,He Y,Wang HF, et al. Conjugation of glucose oxidase onto Mn-doped ZnS quantum dots for phosphorescent sensing of glucose in biological fluids[J]. Anal Chem, 2010, 82(4):1427

[16] Zhang Z, Miao Y, Zhang Q, et al. Facile and sensitive detection of protamine by enhanced room-temperature phosphorescence of Mn-doped ZnS quantum dots[J]. Anal Biochem, 2015, 478:90

[17] 李瑜婷,苗艳明,闫桂琴. 基于 Mn 掺杂 ZnS QDs室温磷光法检测环丙沙星[J]. 分析试验室, 2015, 34(4): 401

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备注/Memo

备注/Memo:

基金项目 国家自然科学基金青年基金资助项目（21205087）
作者简介 王娅（1989-）女，药师，学士，研究方向：纳米材料在药物检测方面的应用；通信作者：高卫真，E-mail:weizhengao33@163.com。



 

更新日期/Last Update: 2018-01-23