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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

24卷

期数:

2018年06期

页码:

517-520

栏目:

临床医学

出版日期:

2018-11-20

文章信息/Info

Title:

Expression and significance of miR-202, AFP and DKK1 in serum of patients with primary hepatic carcinoma

作者:

韩 悦; 魏殿军

（天津医科大学第二医院检验科 ，天津 300211）

Author(s):

HAN Yue ; WEI Dian-jun

（Department of Clinical Laboratory，The Second Hospital, Tianjin Medical University，Tianjin 300211，China）

关键词:

miRNA-202; 甲胎蛋白; 分泌性蛋白Dikkopf-1; 原发性肝癌

Keywords:

miR-202;  alpha fetoprotein;  secretory protein Dikkopf-1;  primary hepatic carcinoma

分类号:

R735.7

DOI:

-

文献标志码:

A

摘要:

目的：研究微小RNA-202（miR-202）、甲胎蛋白（AFP）、分泌性蛋白Dikkopf-1（DKK1）在原发性肝癌（PHC）患者血清中的表达水平及临床价值。方法：选择2013年6月-2014年12月我院肝病科收治的PHC患者（PHC组）43例、良性肝病患者（良性肝病组）37例作为研究对象，选择同期健康体检人员51例作为健康对照组。检测3组受试对象血清中miR-202、AFP及DKK1的表达水平，运用受试者工作特征曲线（ROC曲线）分析miR-202、AFP、DKK1联合检测诊断PHC的诊断效能。结果：（1）PHC患者血清中miR-202水平低于良性肝病组和健康对照组，有显著性差异（P＜0.05）；AFP和DKK1水平均高于良性肝病组和健康对照组，差异具有统计学意义（P＜0.05）。（2）联合检测诊断PHC的效能高于miR-202、AFP及DKK1单独检测。（3）miR-202与AFP呈负相关（r =-0.314，P＜0.05）；miR-202与DKK1呈负相关（r =-0.319，P＜0.05）；AFP与DKK1呈正相关（r=0.556，P＜0.05）。结论：原发性肝癌患者miR-202水平表达水平降低，AFP和DKK1表达水平升高，三者联合检测有助于原发性肝癌的临床诊治。

Abstract:

Objective: To study the expression level and clinical value of tiny RNA-202 (miR-202), alpha fetoprotein (AFP) and secretory protein Dikkopf-1 (DKK1) in serum of patients with primary hepatic carcinoma(PHC). Methods： From June 2013 to December 2014, 43 patients with PHC (PHC group) and 37 patients with benign liver disease (benign liver disease group) were selected as the research subjects. Fifty-one healthy persons during the same period were selected as healthy control group. The levels of serum miR-202, AFP and DKK1 in three groups were detected. Receiver operating characteristic curve (ROC curve) was used to analyze the diagnostic efficiency of miR-202, AFP and DKK1 in the diagnosis of primary hepatic carcinoma. Results：The serum level of miR-202 in PHC group was lower than that in benign liver disease group and healthy control group(P< 0.05). AFP and DKK1 levels were all higher than those in benign liver disease group and healthy control group, and the difference was statistically significant(P< 0.05). The efficacy of joint detection in the diagnosis of PHC was higher than that of miR-202, AFP and DKK1.MiR-202 was negatively correlated with AFP(r=-0.314, P<0.05); miR-202 was negatively correlated with DKK1 (r=-0.319, P< 0.05); AFP was positively correlated with DKK1(r=0.556, P<0.05). Conclusion：The expression levels of miR-202 and AFP and DKK1 could increase in patients with primary hepatic cancer. The combined test of the three may be useful to the clinical diagnosis and treatment of primary hepatic cancer.

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相似文献/References:

[1]徐 飞,牛文彦.甲胎蛋白和甲胎蛋白异质体诊断原发性肝癌的价值[J].天津医科大学学报,2014,20(06):475.

备注/Memo

备注/Memo:

基金项目 天津市卫计委科技基金资助项目（2015KZ101）
作者简介 韩悦（1984-），男，技师，硕士在读，研究方向：临床检验诊断；通信作者：魏殿军，E-mail：weidianjun01@163.com。

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更新日期/Last Update: 2018-11-30