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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

24卷

期数:

2018年05期

页码:

409-414

栏目:

基础医学

出版日期:

2018-09-20

文章信息/Info

Title:

Comparison of the effects of different bioactive materials on mesenchymal stem cells

作者:

刘元媛; 曹晓沧

天津医科大学总医院消化内科，天津300052

Author(s):

LIU Yuan-yuan;  CAO Xiao-cang

Department of Hepato-Gastroenterology, General Hospital, Tianjin Medical University, Tianjin 300052, China

关键词:

水凝胶; 人胎盘来源的间充质干细胞（hP-MSCs）; 调节; 增殖; 凋亡

Keywords:

hydrogel;  human placenta-derived mesenchymal stem cells （hP-MSC）;  regulation;  proliferation; apoptosis

分类号:

R34

DOI:

-

文献标志码:

A

摘要:

目的：研究壳聚糖（CS）及壳聚糖负载的一氧化氮（NO）、胰岛素样生长因子-1C（IGF-1C）与人胎盘来源的间充质干细胞（hP-MSCs）共培养后对细胞功能的影响。方法：利用可稳定表达绿色荧光蛋白（GFP）和萤火虫荧光蛋白（Fluc）的hP-MSCs与3种不同的生物活性材料水凝胶共培养，通过MTT、生物发光成像技术以检测水凝胶对细胞增殖的影响，同时利用real time PCR检测水凝胶对细胞增殖、凋亡、抗炎及血管新生相关细胞因子的表达作用。结果：CS-IGF-1C水凝胶可明显促进hP-MSCs的增殖及上调相关基因IGF-1（11.51）、HGF（6.64）、EGF（9.78）的表达（P< 0.05），抑制hP-MSCs的凋亡并下调相关基因BAX/BAD（1.22/1.12）、FAS/FASLG（1.31/1.70）的表达（P< 0.05），同时还可显著上调hP-MSCs对TSG-6（11.61）及PGE2（16.04）的表达（P< 0.05）。而CS-NO可显著促进hP-MSCs对VEGF（13.28）、PDGF（8.49）的表达作用（P< 0.05）。结论：不同水凝胶对MSCs的影响不同，CS-IGF-1C水凝胶与MSCs共移植可用于治疗组织损伤及炎症性疾病，而CS-NO水凝胶与MSCs共移植可治疗缺血性疾病，为临床及实验室研究提供参考。

Abstract:

Objective: To investigate the effect of chitosan （CS） and chitosan-supported nitric oxide （NO） and insulin-like growth factor-1C （IGF-1C） on placenta-derived mesenchymal stem cells （hP-MSCs） by co-culturing. Methods: HP-MSCs which had stable expression of green fluorescent protein （GFP） and fireflies fluorescent protein （Fluc） were co-cultured with three different bioactive material hydrogels, and the proliferation of hP-MSCs was detected through MTT assay and bioluminescence imaging. Real time PCR was used to detect the relative gene expression of proliferation and apoptosis. Results: In the condition of co-culture with hP-MSCsGFP-Fluc, CS-IGF-1C hydrogel significantly promoted the expression of IGF-1（11.51）, HGF （6.64）, EGF （9.78） and inhibited the expression of apoptosis-related genes BAX/BAD （1.22/1.12） and FAS/FASLG（1.31/1.70）（P<0.05）. At the same time, it significantly promoted the expression of TSG-6（11.61） and PGE2（16.04） by hP-MSCs（P< 0.05）. On the other hand, CS-NO significantly promoted the expression of VEGF（13.28） and PDGF（8.49） from hP-MSCs（P<0.05）. Conclusion:Different hydrogels have different effect on MSCs. Therefore, CS-IGF-1C hydrogel co-transplantated with MSCs would be used in the treatment of tissue damage and inflammatory diseases, and CS-NO hydrogel co-transplantated with MSCs may also be used in the treatment of tissue ischemic disease.

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相似文献/References:

备注/Memo

备注/Memo:

作者简介 刘元媛（1990-），女，硕士在读，研究方向：炎症性肠病与干细胞治疗；通信作者：曹晓沧，E-mali:docxccao@163.com。

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更新日期/Last Update: 2018-09-30