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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

24卷

期数:

2018年05期

页码:

381-384

栏目:

基础医学

出版日期:

2018-09-20

文章信息/Info

Title:

Effect of HaoBieYangYinRuanJian on liver fibrosis induced by TGF-β1

作者:

唐诗慧; 张 丽; 方步武

天津医科大学药理学系，天津300070

Author(s):

TANG Shi-hui; Zhang Li; FANG Bu-wu

Department of Pharmacology，Tianjin Medical University，Tianjin 300070，China

关键词:

肝纤维化; 蒿鳖养阴软坚方; TGF-β1; collagen Ι;  α-SMA

Keywords:

hepatic fibrosis;  HaoBieYangYinRuanJian;  TGF-β1; collagen Ι;  α-SMA

分类号:

R96

DOI:

-

文献标志码:

A

摘要:

目的：研究蒿鳖养阴软坚方在体外对LX-2细胞的影响，从而探讨蒿鳖养阴软坚方对TGF-β1诱导的肝纤维化的作用。方法：体外培养人肝星状细胞LX-2，实验分为正常对照组、TGF-β1刺激组和蒿鳖养阴软坚方低、中、高浓度给药组。MTT法检测LX-2细胞增殖情况；比色法检测细胞培养液中乳酸脱氢酶（LDH）的活性；酶消化法检测细胞上清羟脯氨酸（Hyp）的含量；Western blot法检测collagen Ι以及α-SMA蛋白表达量。结果：TGF-β1作用于LX-2能够明显促进细胞的增殖，collagen I、α-SMA的表达量显著升高。蒿鳖养阴软坚方能抑制LX-2细胞增殖并且能够降低collagen Ι和α-SMA的表达。结论：蒿鳖养阴软坚方能够显著抑制LX-2增殖，下调collagen Ι和α-SMA的表达，降低Hyp生成，从而发挥抗肝纤维化的作用。

Abstract:

Objective: To investigate the effect of HBYYRJ on hepatic stellate cells (HSCs, LX-2) in vitro, and explore the effect of HBYYRJ on liver fibrosis induced TGF-β1. Methods: LX-2 cells were cultured in vitro and divided into control group, TGF-β1 stimulation group and HBYYRJ low dose group, middle dose group and high dose group. The proliferation of LX-2 cells was detected by MTT assay; the activity of LDH was detected by colorimetric assay; and the content of Hyp was detected by enzyme digestion. Western blot was used to detect the protein expression of collagen Ι and α-SMA. Results: TGF-β1 significantly promoted the proliferation of LX-2, TGF-β1 significantly increased the level of collagen Ι and α-SMA. HBYYRJ significantly reduced the proliferation of LX-2, and significantly reduced the level of collagen Ι and α-SMA. Conclusion: HBYYRJ can significantly reduce the proliferation of LX-2, down-regulate the expression of collagen Ι and α-SMA, and decrease the synthesis of Hyp to attenuate hepatic fibrosis.

参考文献/References:

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备注/Memo

备注/Memo:

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更新日期/Last Update: 2018-09-30