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[1]陈妍妍,宋 巍,曹 阳,等.碳青霉烯耐药克雷伯菌耐药机制研究[J].天津医科大学学报,2017,23(06):537-541.
　CHEN Yan-yan,SONG Wei,CAO Yang,et al.Resistance mechanism of carbapenem resistant Klebsiella[J].Journal of Tianjin Medical University,2017,23(06):537-541.
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
23卷
期数:
2017年06期
页码:
537-541
栏目:
临床医学
出版日期:
2017-11-20

文章信息/Info

Title:
Resistance mechanism of carbapenem resistant Klebsiella
文章编号:
1006-8147(2017)06-0537-05
作者:
陈妍妍1 ; 宋 巍2; 曹 阳1; 宗晓龙1 ; 魏殿军1
(1.天津医科大学第二医院检验科,300211;2.天津医科大学总医院院内感染管理科,300052)
Author(s):
CHEN Yan-yan1;  SONG Wei2;  CAO Yang1; ZONG Xiao-long1;  WEI Dian-jun1

(1.Department of Clinical Laboratory, The Second Hospital ， Tianjin Medical University, Tianjin 300211,China; 2.Nosocomial Infection Management Section , General Hospital ，Tianjin Medical University,Tianjin 300050,China)

 

关键词:
青霉烯耐药; 克雷伯菌; 外膜蛋白; 耐药机制
Keywords:
carbapenem resistance;  klebsiella;  outer membrane protein;  mechanism
分类号:
R446
DOI:
-
文献标志码:
A
摘要:
目的：探讨天津医科大学第二医院分离得到的碳青霉烯耐药克雷伯菌的耐药机制。方法：2013-2014年度临床微生物实验室分离到36株碳青霉烯耐药克雷伯菌，改良Hodge试验、EDTA协同试验及 PCR法检测碳青霉烯酶耐药基因，PCR法检测ESBLs及Ampc酶耐药基因，PCR、实时荧光定量PCR（RT-PCR）法检测外膜蛋白基因Ompk35及Ompk36存在及表达情况。结果：9株改良Hodge试验阳性，EDTA协同试验均阴性，碳青霉烯酶耐药基因均阴性；36株碳青霉烯耐药克雷伯菌至少存在1种β内酰胺酶耐药基因；外膜蛋白基因无缺失，33株存在外膜蛋白表达量下降。结论： 分离的碳青霉烯耐药克雷伯菌耐药机制可能为ESBLs及Ampc酶表达合并外膜蛋白表达下降。
Abstract:
Objective: To investigate the mechanism of carbapenem resistant Klebsiella. Methods: Thirty-six carbapenem resistant Klebsiella were collected from 2013 to 2014. Modified Hodge test, EDTA synergy test and PCR were applied to detect genes of ESBLs and Ampc carbapenemases. PCR and RT-PCR test were conducted to detect gene of outer membrane protein Ompk35 and Ompk36 and their expressions. Results: Nine strains were found by modified Hodge test positive. EDTA co-test results were negative. Carbapenemase resistance genes detection results were all negative. Thirty-six strains of carbapenem resistant Klebsiella showed at least one β-lactamase resistance gene; There was no deletion of membrane protein gene, and 33 cases showed decreased expression of outer membrane protein. Conclusion: The probable drug-resistant mechanism of the Klebsiella isolated is the combined expression of the ESBLs and Ampc enzyme and may decrease expression of out membrane protein.

参考文献/References:

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相似文献/References:

备注/Memo

备注/Memo:

作者简介 陈妍妍（1989-），女，硕士在读，研究方向：医院感染及分子流行病学研究；

通信作者：魏殿军 ，E-mail：weidianjun01@163.com。



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更新日期/Last Update: 2017-11-14