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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

23卷

期数:

2017年05期

页码:

394-397

栏目:

基础医学

出版日期:

2017-09-20

文章信息/Info

Title:

Knee loading promotes osteointegration in osteoporosis rats

文章编号:

1006-8147(2017)05-0394-04

作者:

张云龙¹; 2; 王 健²; 陈 刚²; 王钊楠¹; 刘大全¹; 李心乐¹; 张 平¹

（1. 天津医科大学人体解剖与组织胚胎学系,天津 300070; 2.天津医科大学口腔医学院口腔颌面外科,天津 300070）

Author(s):

ZHANG Yun-long¹; 2;  WANG Jian²;  CHEN Gang²;  WANG Zhao-nan¹;  LIU Da-quan¹; LI Xin-le¹;  ZHANG Ping¹

(1.Department of Anatomy and Histology, Tianjin Medical University, Tianjin 300070, China; 2.Department of Oral and Maxillofacial Surgery，School of Stomatology, Tianjin Medical University, Tianjin 300070, China)

关键词:

机械加载; 骨质疏松; 骨结合; 骨密度; 生物力学

Keywords:

knee loading;  osteoporosis;  osteointegration;  bone mineral density;  mechanical test

分类号:

R78

DOI:

-

文献标志码:

A

摘要:

目的：利用骨质疏松实验动物模型，探讨机械加载促进种植体周围骨结合的效能。方法：12只SD雌性大鼠（12周龄）卵巢切除建立绝经后骨质疏松动物模型（OVX)，另12只动物设为对照。4周后所有动物均于双侧股骨中段植入直径1.5 mm,长度4.5 mm的纯钛种植体。按术式分别随机分组如下：假手术组（sham)、假手术膝关节加载治疗组(sham+loading)、OVX模型组(OVX)和OVX膝关节加载治疗组(OVX+loading)(n =6)。种植术后第2天对双侧膝关节进行机械加载刺激（5 N，10 Hz，3 min/侧，共6 min/d，加载5 d，休息2 d，往复循环4周）。通过卵巢切除前和机械加载后测量的骨密度（BMD）和骨矿化度（BMC）、生物力学测试对治疗效果进行评估。结果：Sham+loading组与sham组相比，膝关节机械加载显著改善BMD和BMC(P＜0.001)。OVX+loading组与 OVX对照组相比，膝关节机械加载明显增加BMD和BMC(P＜0.001)。生物力学测试表明，Sham+loading组与sham组相比，膝关节机械加载显著改善种植体最大推出力(P＜0.001)。OVX+loading组与 OVX对照组相比，膝关节机械加载明显增加种植体最大推出力(P＜0.001)。结论：机械加载显著改善了骨质疏松动物的骨密度，促进骨质疏松大鼠种植体周围的骨结合。该研究对口腔临床种植修复开辟了新思路。

Abstract:

Objective: To investigate whether mechanical loadings can promote osteointegration for dental implant. Methods: Twenty four female Sprague Dawley rats (12 weeks of age) were randomly assigned to 4 groups: sham operated control group (sham), knee loading treated sham operated group (sham+loading), ovariectomized group (OVX), and knee loading treated ovariectomized group (OVX+ loading) (n=6 for each group). Four weeks after ovariectomy or sham operation, these rats received secondary surgical intervention to insert the titanium implant (1.5 mm in diameter, 4.5 mm in length) into the bilateral femoral diaphysis. Knee loading was applied since the second postoperative day after implantation. The loading frequency was 10 Hz, and the loading force was 5 N. Loadings were applied for 6 mins per day for five consecutive days per week on bilateral knee joint for 4 weeks. The animals’ bone mineral density (BMD) and bone mineral content (BMC) were measured by pDEXA before surgery and sacrifice. The femora then were evaluated by mechanical test. Results: Images with pDEXA revealed that BMD and BMC in the loaded OVX group were significantly increased compared with OVX group (both P<0.001). Mechanical test showed that maximum pullout force of the femora were significantly increased by knee loading (P<0.001). Conclusion: This study demonstrated that knee loading could accelerate osteointegration by the implant in the femoral midshaft in osteoporosis SD rats. Mechanical loading could become a potential therapeutic model for dental implants in osteoporosis patients.

参考文献/References:

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备注/Memo

备注/Memo:

基金项目 国家自然科学基金资助项目（81572100）；天津市应用基础与前沿技术研究计划重点项目（14JCZDJC36500）；国家自然科学基金资助项目（81601863）

作者简介 张云龙（1990-），男，硕士在读，研究方向：种植体骨结合的研究；通信作者：张平，E-mail：pizhang2008@163.com; 陈刚，E-mail：doctorchen@live.cn 。

常用功能

更新日期/Last Update: 2017-09-20