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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

23卷

期数:

2017年05期

页码:

403-407

栏目:

基础医学

出版日期:

2017-09-20

文章信息/Info

Title:

Effects of endothelial progenitor cells on dorsal root ganglion neurites and the expression of Nogo-A and NgR

文章编号:

1006-8147(2017)05-0403-05

作者:

李文辉¹; 张 亮¹; 张孝安¹; 吕小琴¹; 柳 艳¹; 周家强²; 王 凯¹

（1.天津医科大学第二医院骨科，天津300211；2.天津医科大学总医院滨海医院骨科，天津300480）

Author(s):

LI Wen-hui¹;  ZHANG Liang¹;  ZHANG Xiao-an¹;  Lü Xiao-qin¹;  LIU Yan¹;  ZHOU Jia-qiang²;  WANG Kai¹

(1.Department of Orthopedics, The Second Hospital, Tianjin Medical University, Tianjin 300211, China; 2.Department of Orthopedics, Bing Hai Hospital, Tianjin Medical University General Hospital, Tianjin 300480, China)

关键词:

内皮祖细胞; 背根神经节; 共培养; 细胞突起; Nogo-A;  NgR

Keywords:

endothelial progenitor cells;  dorsal root ganglion;  co-culture;  neurite;  Nogo-A;  NgR

分类号:

R651.3

DOI:

-

文献标志码:

A

摘要:

目的 :观察大鼠内皮祖细胞（EPCs）对乳鼠背根神经节（DRG）细胞突起影响，通过Nogo-A和NgR表达变化探究其可能机制。方法:培养DRG和制备EPCs后分别鉴定，实验组DRG和EPCs共培养，对照组2份等量的DRG共培养。第2和4日分别计算两组DRG突起数量,突起最长和平均长度,统计两组DRG细胞纯度和活性，检测Nogo-A和NgR及mRNA表达。结果:实验组DRG细胞突起数量、突起最长和平均长度在共培养第2和4日较对照组均明显增长（P＜0.05）,实验组DRG细胞纯度和活性均高于对照组（P＜0.05），实验组Nogo-A和NgR mRNA表达量在共培养第2和4日均显著降低（P＜0.05），Western Blot结果示实验组Nogo-A和NgR蛋白表达量在第2和4日明显低于对照组（P＜0.05）。结论:EPCs通过介导Nogo-A和NgR表达下调，促进DRG细胞突起生长。

Abstract:

Objective: To explore the effect of endothelial progenitor cells (EPCs) on dorsal root ganglions (DRG) neurites by the expression of Nogo-A and NgR. Methods: DRG and the EPCs were prepared and identified separately. EPCs co-culturing with DRG was designed as the experimental group, and DRG co-culturing with DRG was designed as the control group. The number of neurites, the maximum length of neurites, the average length, the purity and vitality were calculated by Image J software on day 2 and day 4. Correspondingly Nogo-A, NgR mRNA and protein were detected. Results: The number of DRG neurites, the maximum length of DRG neurites and the average length elevated dramatically (P<0.05). The purity and vitality of DRG in experimental group were significantly higher (P<0.05). Nogo-A and NgR mRNA in experimental group were significantly lower on day 2 and day 4 (P<0.05). Corresponding protein expression of Nogo-A and NgR in experimental group were significantly lower (P<0.05). Conclusion: EPCs could restraint the expression of Nogo-A and NgR, which may markedly promote the growth of DRG neurites.

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相似文献/References:

备注/Memo

备注/Memo:

基金项目 天津市卫生局科技基金资助（2014KZ038）

作者简介 李文辉（1987-），男，硕士在读，研究方向：脊髓损伤；

通信作者：王凯，E-mail: wangkaiy48@126.com。

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更新日期/Last Update: 2017-09-20