|本期目录/Table of Contents|

[1]樊保佑,林伟,史桂东,等.探究激活态雪旺细胞与生物可降解聚己内酯支架的生物相容性[J].天津医科大学学报,2017,23(05):398-402.
　FAN Bao-you,LIN Wei,SHI Gui-dong,et al.Study on the biocompatibility of PCL scaffold with activated Schwann cells[J].Journal of Tianjin Medical University,2017,23(05):398-402.
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探究激活态雪旺细胞与生物可降解聚己内酯支架的生物相容性()
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
23卷
期数:
2017年05期
页码:
398-402
栏目:
基础医学
出版日期:
2017-09-20

文章信息/Info

Title:
Study on the biocompatibility of PCL scaffold with activated Schwann cells
文章编号:
1006-8147(2017)05-0398-05
作者:
樊保佑; 林伟; 史桂东; 任一鸣; 魏志坚; 郝岩; 周先虎; 冯世庆
（天津医科大学总医院骨科，天津 300052）

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Author(s):
FAN Bao-you;  LIN Wei;  SHI Gui-dong;  REN Yi-ming;  WEI Zhi-jian;  HAO Yan;  ZHOU Xian-hu;  FENG Shi-qing
(Department of Orthopedics, General Hospital ，Tianjin Medical University,Tianjin, China, 300052)
关键词:
激活态雪旺细胞; PCL支架; 生物相容性
Keywords:
activatedschwann cells;  polycaprolactone scaffold; biocompatibility
分类号:
R68
DOI:
-
文献标志码:
A
摘要:
目的 :探究激活态雪旺细胞（ASC）与非激活态雪旺细胞（SC）与生物可降解聚己内酯（PCL）支架的生物相容性。方法:实验组由结扎7 d Wistar大鼠坐骨神经提取激活态雪旺细胞，对照组由未结扎坐骨神经提取非激活态雪旺细胞。将细胞分别种植在PCL支架上，通过CCK-8检测细胞增殖活性，通过结晶紫观察细胞分布，激光共聚焦显微镜观察细胞形态和功能表达。结果: ASC与SC相比S-100阳性率无明显差异；PCL支架上ASC增殖率明显高于SC；ASC能够贴附PCL纤维生长，可沿纤维长轴分布，进入支架内部，并且表达髓鞘碱性蛋白（MBP）。结论:激活态雪旺细胞比非激活态雪旺细胞能够更好地在PCL支架上生长，PCL支架具有良好的生物相容性。
Abstract:
Objective: To study the biocompatibility of polycaprolactone scaffold with activated Schwann cells(ASC)and normal Schwann cells(SC). Methods:There were two groups: ASC extracted from ligated sciatic nerves experiment group; SC extracted from normal sciatic nerves control group. Proliferation activity was tested through the CCK- 8 test, and neurotrophic factors was dectected by real-time PCR and the distribution and shape of cells was observed by crystal violet staining and confocal laser scanning microscope. Results: There was no difference between ASC and SC in the positive rate of S-100; the proliferation rate of ASC was significantly higher than that of SC in PCL; ASC could attach PCL fiber and move along the long axis of the fiber into the scaffolds, and express myelin basic protein (MBP). Conclusion:Activated Schwann cells can grow better in PCL scaffold than non-activated Schwann cells, and PCL scaffold has good biocompatibility.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目国家自然科学基金重点项目基金资助（81330042）；国家自然科学基金面上项目（81371957）；国家科技部中俄合作专项项目（2014DFR31210）；国家自然科学基金国际合作与交流项目（81620108018）；天津市科委重点项目(14ZCZDSY00044，13RCGFSY19000)

作者简介樊保佑（1990-），男，硕士在读，研究方向：脊髓损伤；

通信作者：冯世庆， E-mail：sqfeng@tmu.edu.cn。

更新日期/Last Update: 2017-09-20