|本期目录/Table of Contents|

[1]刘双庆,李 娟,陈妍妍,等.肠外致病性大肠埃希菌毒力与耐药的相关性分析[J].天津医科大学学报,2017,23(04):349-353.
　LIU Shuang-qing,LI Juan,CHEN Yan-yan,et al.Association of virulence and resistance of extraintestinal pathogenic E.coli[J].Journal of Tianjin Medical University,2017,23(04):349-353.
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肠外致病性大肠埃希菌毒力与耐药的相关性分析(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
23卷
期数:
2017年04期
页码:
349-353
栏目:
临床医学
出版日期:
2017-07-02

文章信息/Info

Title:
Association of virulence and resistance of extraintestinal pathogenic E.coli
文章编号:
1006-8147(2017)04-0349-05
作者:
刘双庆1; 李 娟2; 陈妍妍1; 曹 阳1; 魏殿军1
（1.天津医科大学第二医院检验科，天津 300211；2.中国人民武装警察部队后勤学院附属医院呼吸和重症医学科，天津 300162）
Author(s):
LIU Shuang-qing1;  LI Juan2;  CHEN Yan-yan1; CAO Yang1;  WEI Dian-jun1
(1.Department of Clinical Laboratory, The Second Hospital ， Tianjin Medical University, Tianjin 300211，China; 2.Department of Respiratory and Critical Care Medicine, Affiliated Hospital of Logistics University of Chinese People’s Armed Police Force,Tianjin 300162，China)
关键词:
肠外致病性大肠埃希菌; 毒力因子; Logistic 回归; 适应性代价
Keywords:
extraintestinal pathogenic E.coli;  Virulence factors;  Logistic regression;  fitness cost
分类号:
R446
DOI:
-
文献标志码:
A
摘要:
目的：通过检测不同来源肠外致病性大肠埃希菌（ExPEC）多种毒力因子基因检出情况及耐药情况，初步探讨两者相关性，了解 ExPEC 毒力与耐药相互关系。方法：187 株分离自尿液及其他部位的菌株，分别检测其毒力因子基因的存在情况及耐药情况，χ2 检验比较不同来源菌株毒力因子基因检出率及耐药率有否差异，Logistic 回归模型分析菌株毒力因子检出率与菌株耐药率相关性。结果：ExPEC 中毒力基因 kpsMT II、papC、PapEF、papG allele II (Internal)、papA、cnf1、sfa/focDE、K5 和 rfc 在非尿液来源比尿液来源检出率更高；尿液来源环丙沙星、左旋氧氟沙星和哌拉西林/他唑巴坦的耐药情况均显著高于非尿液来源菌株；Logistic 回归模型分析表明共有 11 种毒力基因与其相对应的抗菌药物耐药有相关性，其中 9 种毒力基因抑制菌株耐药发生，1 种毒力基因促进菌株耐药发生，另有 1 种毒力基因在不同药物中有相反作用。结论：ExPEC 由于受到环境与抗菌药物的选择压力不同，其毒力因子检出情况及耐药情况也会有差异，ExPEC 毒力因子与菌株耐药情况关系密切。
Abstract:
Objective ：To investigate the association of extra-intestinal pathogenic E.coli (ExPEC) clinical isolates and resistance. Methods：A total of 187 ExPEC clinical isolates were investigated. Multiplex PCR and the minimum inhibitory concentrations (MICs) of antimicrobials were performed to detect the virulence factors and resistance among the 187 E. coli strains isolates. The comparisons of proportions between groups were carried out by using χ2 test and Logistic regression. Results：The virulence factor genes kpsMT II, papC, PapEF, papG allele II (Internal), papA, cnf1, sfa/focDE, K5 and rfc had higher detection rates in non-urinary tract group than urinary tract group. The antimicrobial drugs CIP, LVX and TZP had higher resistance rates in urinary tract group than non-urinary tract group. Logistic regression analysis showed 9 kinds of virulence factor genes detected were independent protective factors for resistance of ExPEC, and one was independent risk factors for resistance of ExPEC. And another one had the two functions. Conclusion：Due to the different microenvironment and the selection pressure of antibiotics, the detection rate of virulence factor genes and the resistance rate of ExPEC are different, and the two are closely related.

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备注/Memo

备注/Memo:
作者简介 刘双庆（1988-），男，初级技师，硕士，研究方向：感染性疾病分子流行病学；

通信作者：魏殿军， E-mail：dianjunwei@tmu.edu.cn。

更新日期/Last Update: 2017-07-01