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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

23卷

期数:

2017年04期

页码:

295-299

栏目:

基础医学

出版日期:

2017-07-02

文章信息/Info

Title:

Effects of IL-23 on the herin proliferation，invasion and bi-differentiation? of melanoma cells

文章编号:

1006-8147(2017)04-0295-05

作者:

文明明¹; 马跃美¹; 赵秀兰²; 3; 张丹芳²; 赵 楠²; 李岩磊²; 林 贤²; 董学易²; 刘 芳²

（1.天津医科大学外科手术学教研室，天津 300070；2.天津医科大学病理学教研室，天津 300070；3.天津医科大学总医院病理科，天津 300052 ）

Author(s):

WEN Ming-ming¹; MA Yue-mei¹ ; ZHAO Xiu-lan²; 3;  ZHANG Dan-fang²; ZHAO Nan ²;  LI Yan-lei²;  LIN Xian²;  DONG Xue-yi²;  LIU Fang²

（1.Department of Operative Surgery, Tianjin Medical University, Tianjin 300070, China；2.Department of Pathology, Tianjin Medical University, Tianjin 300070, China； 3.Department of Pathology, General Hospital ， Tianjin Medical University, Tianjin 300052, China）

关键词:

黑色素瘤; IL-23; 增殖; 迁移; 侵袭

Keywords:

malignant melanoma; IL-23;  proliferation; migration;  invasion

分类号:

R36

DOI:

-

文献标志码:

A

摘要:

目的：检测白细胞介素-23（IL-23）在黑色素瘤中的表达并探讨IL-23 对黑色素瘤细胞双向分化及增殖、侵袭能力的影响。方法：将IL-23表达质粒转染至黑色素瘤细胞系MUM-2B中，使MUM-2B中IL-23外源性降表达,观察IL-23转染前、后MUM-2B细胞形态学改变；MTT、细胞划痕和Transwell侵袭实验检测对增殖、迁移及侵袭能力的影响；Western blot检测转染前、后MUM-2B中IL-23、相关蛋白（Vimentin、Snail、N-cadherin和MMP-2）表达变化情况，免疫荧光检测IL-23、Vimentin和MMP-2表达部位，明胶酶谱检测MMP-2，MMP-9活性。结果：转染后MUM-2B的细胞形态发生明显改变。细胞的增殖、迁移及侵袭能力显著减弱。IL-23降表达，N-cad、Vimentin、NF-κβ、Snail和MMP-2表达明显下调,MMP-2，MMP-9活性显著降低。结论：IL-23 可促进黑色素瘤细胞向间叶样表型转变，并促进黑色素瘤的增殖、迁移和侵袭。

Abstract:

Objective:To examine the expression of IL-23 in melanoma cells and its effecton proliferation，invasion and bi-differentiation.

Methods: To establish stable IL-23 knockdown cells, MUM-2B cells were stably transfected with sh-IL-23 and sh-Control vectors.The changes of MUM-2B cells morphology were detected after IL-23transfection by using microscope;The proliferation of MUM-2Bcells was determined by MTT,the migration ofMUM-2B cells was determined by the wound healing assay. And the invasion of MUM-2B cells was detected by Transwellexperiment.The changes of the expression of related protein（Vimentin，Snail，N-cadherin and MMP-2）in MUM-2Bcells were detected by Western blot after transfection ofIL-23;Using immunofluorescence， the sites of the expression of related protein（IL-23,Vimentin and MMP-2） were detected；The expression activities of MMP-2 and MMP-9 were analyzed by Gelatin Zymography. Results:After IL-23 knockdown, the morphology ofMUM-2B cells showed significant changes; IL-23 knockdown significantly decreased the abilities of MUM-2B cells to proliferate ,invade and migrate;Compared with MUM-2B-Control cells, knockdown expression of IL-23 significantly down-regulated N-cad, Vimentin, NF-κβ,Snailand MMP-2. Importantly, MUM-2B cells knockdown IL-23 demonstrated decreasedtheexpressionactivities of MMP-2 and MMP-9.Conclusion: IL-23 contributes to transfection of mesenchymal-like phenotypeand facilitates the proliferation,migration and invasionof melanoma cells.

参考文献/References:

[1] Caini S, Boniol M, Botteri E, et al. The risk of developing a second primary cancer in melanoma patients: a comprehensive review of the literature and meta-analysis[J]. J Dermatol Sci, 2014, 75(1): 3

[2] Zbytek B, Carlson JA, Granese J, et al. Current concepts of metastasis in melanoma[J]. Expert Rev Dermatol, 2008. 3(5): 569

[3] 杨举伦. 双向分化肿瘤的病理诊断[J]. 诊断病理学杂志, 1997, 4(3): 164

[4] Schreiber RD, Old LJ, Smyth MJ. Cancer immunoediting: integrating immunity’s roles in cancer suppression and promotion[J]. Science. 2011, 331(6024): 1565

[5] Feng S, Chen XM, Wang JF, et al. Th17 cells associated cytokines and cancer[J]. Eur Rev Med Pharmacol Sci, 2016, 20(19): 4032

[6] von SB, Leung PS, Yong MC, et al. Combined anti-CD40 and anti-IL-23 monoclonal antibody therapy effectively suppresses tumor growth and metastases[J]. Cancer Res, 2014, 74(9): 2412

[7] Lyakh L, Trinchieri G, Provezza L,et al. Regulation of interleukin-12/interleukin-23 production and the T-helper 17 response in humans[J]. Immunol Rev, 2008, 226: 112

[8] Boniface K, Blom B, Liu YJ, de Waal Malefyt R. From interleukin-23 to T-helper 17 cells: human T-helper cell differentiation revisited[J]. Immunol Rev, 2008, 226: 132

[9] 赵尔增, 岳茂兴. 肛管直肠原发性恶性黑色素瘤临床病理特点[J]. 世界华人消化杂志, 2004, 12(2): 225

[10] 纪小龙, 申明识. 黏膜黑色素瘤的常见临床病理特点[J]. 诊断病理学杂志, 2002 ,9 (2): 50

[11] Keibel A, Singh V, Sharma MC. Inflammation, microenvironment, and the immune system in cancer progression[J]. Curr Pharm Des, 2009, 15(17): 1949

[12] Atsumi T, Singh R, Sabharwal L, et al. Inflammation amplifier, a new paradigm in cancer biology[J]. Cancer Res, 2014, 74(1): 8

[13] Capece D, Fischietti M, Verzella D, et al. The inflammatory microenvironment in hepatocellular carcinoma: a pivotal role for tumor-associated macrophages[J]. Biomed Res Int, 2013, 2013: 187204

[14] Langowski JL, Zhang X, Wu L, et al. IL-23 promotes tumour incidence and growth[J]. Nature, 2006,442(7101): 461

[15] Zhang L, Li J, Li L, et al. IL-23 selectively promotes the metastasis of colorectal carcinoma cells with impaired Socs3 expression via the STAT5 pathway[J]. Carcinogenesis, 2014, 35(6): 1330

[16] Seftor RE, Seftor EA, Koshikawa N, et al. Cooperative interactions of laminin 5 gamma2 chain, matrix metalloproteinase-2, and membrane type-1-matrix/metalloproteinase are required for mimicry of embryonic vasculogenesis by aggressive melanoma[J]. Cancer Res, 2001, 61(17): 637

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备注/Memo

备注/Memo:

基金项目 国家自然科学基金面上项目基金资助(81572872)

作者简介 文明明（1989-），男，硕士在读，研究方向：炎症与肿瘤血管生成关系的研究；

通信作者：马跃美，E-mail:maym@tmu.edu.cn。

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更新日期/Last Update: 2017-06-30