[1]薛茜,陈虹,高颖,等.HPLC测定复方甘草胶囊浸膏中苦参碱、氧化苦参碱含量[J].天津医科大学学报,2017,23(03):277-279.
　XUE Xi,CHEN Hong,GAO Ying,et al.?Determination of contents of matrine and oxymatrine in extraction of compound liquorice capsules by HPLC[J].Journal of Tianjin Medical University,2017,23(03):277-279.
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HPLC测定复方甘草胶囊浸膏中苦参碱、氧化苦参碱含量(PDF)

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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

Title:: ?Determination of contents of matrine and oxymatrine in extraction of compound liquorice capsules by HPLC

作者:: 薛茜¹; 3; 陈虹²; 高颖²; 赵瑞顺³; 张靖婷³; (1.天津医科大学研究生院,天津300070;2.武警后勤学院生药学和药剂学教研室,天津300309;3.武警后勤学院附属医院药材科,天津 300162)

Author(s):: XUE Xi¹; 3; CHEN Hong²; GAO Ying²; ZHAO Rui-shun³; ZHANG Jing-ting³; (1.Graduate School,Tianjin Medical University,Tianjin 300070,China; 2.Department of Pharmacognosy and Pharmacy,Logistics University of People^,s Armed Police Force, Tianjin 300309,China;3.Department of Pharmacy,Affiliated Hospital of Logistics University of Peoples Armed Police Force, Tianjin 300162,China)

Keywords:: HPLC; compound liquorice capsules ; matrine; oxymatrine; content determination

摘要:: 目的：建立HPLC同时测定复方甘草胶囊中间体浸膏中苦参碱和氧化苦参碱的含量，为制备胶囊提供依据。方法：色谱柱为Kromasil-NH₂柱（250 mm×4.6 mm, 5 μm）,流动相为乙腈-无水乙醇-3%磷酸（80：10：10 V/V/V）,流速1.0 mL/min,检测波长220 nm，柱温30 ℃。结果：苦参碱在5.12 ~ 30.72 μg/mL(r=0.999 5),氧化苦参碱在5.16 ~ 30.96 μg/mL（r=0.999 5）浓度范围内与峰面积线性关系良好，苦参碱平均回收率99.63%,RSD为0.62%（n=6）,氧化苦参碱平均回收率99.81%，RSD为0.72%（n=6）。结论：该方法准确，稳定性好，重复性高，可用于复方甘草胶囊中间体浸膏的质量控制，为胶囊制备提供依据。

Abstract:: Objective: To establish an HPLC method for the determination of matrine and oxymatrine in extraction of compound Liquorice capsules，and to provide the foundation to prepare capsules. Methods: The HPLC was carried out on Kromasil-NH₂column(250 mm×4.6 mm, 5 μm）, and mobil phase was acetonitrile-absolute alcohol-3%phosphoric acid(80:10:10,V/V/V).The flow rate was 1.0 mL/min.Detection wavelength was 220 nm.The temperature of column was 30 ℃. Results: The linear rangers of matrine was 5.12 - 30.72 μg/mL(r=0.999 5) ,and oxymatrine was 5.16 - 30.96 μg/mL（r=0.999 5）.The average recovery rate of matrine was 99.63% with RSD 0.62%(n=6),and oxymatrine was 99.81% with RSD 0.72% (n=6).Conclusion：This method is accurate, stable and repeatable. It can be used to control the quality of extraction of compound Liquorice capsules ,and to provide the basis to prepare capsules.

[1] 张少少，安银岭，华燕，等.苦参化学成分和药理研究现状[J].中国民族民间医药，2008,8（2）：8

[2] 王俊学，王国俊.苦参碱及氧化苦参碱的药理作用及临床应用[J].肝脏，2000,5（2）：116

[3] 曾以旺，陈燕瑞，王少平，等.HPLC同时测定复方苦参片中苦参碱、氧化苦参碱含量[J].辽宁中医药大学学报,2014,16(11):40

[4] 刘军玲.复方苦参注射液含量测定方法研究[J].安徽医药，2011,15(12):1516

[5] 刘小琴，范华均，黄晓文，等.微波辅助提取高效液相色谱法测定苦参中5中生物碱的含量[J].理化实验-化学分册，2012（3）：299

[6] 刘羽.不同产地和不同季节苦参中苦参碱和氧化苦参碱的含量测定[J].山东化工，2016，16（45）：72

[7] 洪世忠.复方苦参注射液中苦参碱和氧化苦参碱的总含量测定[J].中医学报，2015，7（30）：1014

[8] 周俊，彭翠香，范茜茜，等.益母妇宁胶囊中苦参碱、氧化苦参碱的含量测定[J].湖北中医药大学学报，2015，3（17）：32

[1]陈妍,邢正英,李继彬,等.HPLC法测定黄丹兰颗粒剂中苦参碱和氧化苦参碱含量[J].天津医科大学学报,2016,22(05):452.

备注/Memo:: 基金项目国家重大新药创新项目基金资助(2012ZX09103201-29)
作者简介薛茜（1984-），女，中药师，硕士在读，研究方向：药物化学；通信作者：陈虹，E-mail:chenhongtian06@163.com。
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更新日期/Last Update: 2017-05-18