子查询返回的值不止一个。当子查询跟随在 =、!=、<、<=、>、>= 之后,或子查询用作表达式时,这种情况是不允许的。 B7.1/GM-CSF修饰的PC3-DC融合疫苗在体外抗肿瘤免疫效应-《天津医科大学学报》
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[1].B7.1/GM-CSF修饰的PC3-DC融合疫苗在体外抗肿瘤免疫效应[J].天津医科大学学报,2017,23(03):194-198.
 HAO Xiao-dong,LI Chang-ying,BO Zhi-qiang,et al. Antitumor effect of PC3-DC fusion vaccines modified by B7.1 / GM- CSF in vitro[J].Journal of Tianjin Medical University,2017,23(03):194-198.
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B7.1/GM-CSF修饰的PC3-DC融合疫苗在体外抗肿瘤免疫效应(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
23卷
期数:
2017年03期
页码:
194-198
栏目:
基础医学
出版日期:
2017-05-18

文章信息/Info

Title:
 Antitumor effect of PC3-DC fusion vaccines modified by B7.1 / GM- CSF in vitro
文章编号:
1006-8147(2017)03-0194-05
作者:
-
(1天津医科大学第二医院泌尿外科,天津 300211;2.天津市泌尿外科研究所,天津 300211)
Author(s):
HAO Xiao-dong12LI Chang-ying2BO Zhi-qiang12DING Hao12ZHANG Chang-wen12ZHANG Zhi-hong12
(1 . Department of Urology, The Second Hospital, Tianjin Medical University, Tianjin 300211,China; 2: Tianjin Institute of Urology, Tianjin 300211,China )
关键词:
DC细胞前列腺癌细胞融合疫苗B7.1GM-CSF免疫治疗
Keywords:
dendritic cell prostate cancer fusion vaccine B7.1 GM-CSF Immunotherapy
分类号:
R737.25
DOI:
-
文献标志码:
A
摘要:
 目的:制作B7.1/GM-CSF修饰的DC融合疫苗,并观察其在体外的特异性抗肿瘤免疫效应。方法:制备完整表达GM-CSF的PC3细胞,从前列腺癌患者外周血中分离DC,应用电融合法融合DC和GM-CSF-PC3,构建B7.1/GPI蛋白并锚定于融合细胞表面;应用免疫荧光观察融合细胞形态及锚定稳定性;流式细胞进行融合细胞表形测定;ELISA试剂盒检测IFN-γ分泌情况;MTT及LDH试剂盒测定融合细胞刺激同源异体T淋巴细胞增殖和细胞毒性效应。结果:成功制备GM-CSF-PC3细胞及B7.1/GPI蛋白, 成功分离DC并表达DC分子标记;B7.1/GM-CSF融合疫苗高表达DC分子标记及前列腺癌标记;T细胞增值实验证明融合疫苗具有强烈的免疫刺激活性;细胞毒性实验证明了融合细胞具有比对照组更有效的肿瘤杀伤作用。 结论:电融合技术可成功诱导DC与GM-CSF-PC3融合,体外试验中特定修饰的融合疫苗可显著的提升抗肿瘤免疫效应。
Abstract:
 Objective: To construct the fusion vaccine with dendritic cells and prostate cancer cells (PC3) and modify it by B7.1/GM-CSF, and test the anti-tumor immunological reaction of the fusion vaccine in vitro. Methods: Fusion cells was electrofusioned by DC and GM-CSF-PC3, and B7.1/GPI protein was constructed and anchored to the fusion cell surface; Cell morphology and anchoring stability were determined by immunofluorescence, and fusion cell phenotypic were determined by flow cytometry; fusion vaccines stimulated homologous proliferation and cytotoxic effect of allogeneic T lymphocytes were determined by MTT and LDH kit. Results: B7.1/GM-CSF fusion vaccine highly expressed DC molecular markers and prostate cancer markers; B7.1/GM-CSF fusion vaccines strongly promoted the proliferation of T cells and induced a significantly tumor-specific cytotoxic T lymphocyte (CTL) response and killed the PC3 cells effectively in vitro. Conclusion: the DC and GM-CSF-PC3 can be successfully fused by electrical fusion technique, and the specific modified fusion vaccine can significantly enhance the anti-tumor immune effect in vitro.

参考文献/References:

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备注/Memo

备注/Memo:

基金项目 国家自然科学基金资助项目(81301949);天津市自然科学基金资助项目(16jcybjc26500)

作者简介 郝晓东( 1991-), 男 ,硕士在读,研究方向: 泌尿外科疾病 ;

通信作者:张志宏 ,E-mail:zhihongzhang1967@163.com



 

更新日期/Last Update: 2017-05-18