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[1]白 洁,李光明,王金鑫,等.体外合成JSRV mRNA及其体内表达特异性中和抗体的研究[J].天津医科大学学报,2017,23(03):185-188.
　BAI Jie,LI Guang-ming,WANG Jin-xin,et al.synthesis JSRV mRNA in vitro and its expression of specific antibodies in mouse[J].Journal of Tianjin Medical University,2017,23(03):185-188.
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体外合成JSRV mRNA及其体内表达特异性中和抗体的研究(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
23卷
期数:
2017年03期
页码:
185-188
栏目:
基础医学
出版日期:
2017-05-18

文章信息/Info

Title:
synthesis JSRV mRNA in vitro and its expression of specific antibodies in mouse
文章编号:
1006-8147(2017)03-0185-04
作者:
白 洁1; 李光明1; 王金鑫1; 宋紫暄1; 吴志敏2; 高雁3; 朱 泽1
（1.天津医科大学病原生物学教研室，天津300070；2.武警广西总队医院检验科，南宁530021；3.天津市美联博科技有限公司，天津300221）
Author(s):
BAI Jie1; LI Guang-ming1; WANG Jin-xin1;  SONG Zi-xuan1;  WU Zhi-min2; GaoYan3 ; ZHU Ze1
(1.Department of Microbiology, Tianjin Medical University, Tianjin 300070, China; 2.Clinical Laboratory, Armed Police Corps Hospital of Guangxi, Nanning 530021, China; 3.Tianjin Mei Lian Bo Technology Company, Tianjin 300221，China)
关键词:
中和抗体; 体外合成mRNA; 加亚嘉西科逆转录病毒; 病毒囊膜基因; 小鼠
Keywords:
neutralizingantibody; IVTmRNA; jaagsiekteretrovirus; env;  mouse
分类号:
Q7
DOI:
-
文献标志码:
A
摘要:
目的：应用假病毒中和法检测体外合成JSRV-env mRNA免疫小鼠产生的特异性抗体。方法：优化PT7TS载体序列，插入目的序列经电泳测序验证后，体外转录合成JSRV-env mRNA，将mRNA与鱼精蛋白结合后皮下注射免疫BALB/c小鼠。采用慢病毒包装系统制作JSRV假病毒，测定假病毒滴度，取不同组免疫小鼠血清进行假病毒为基础的抗体中和试验，检测小鼠体内针对JSRV-env mRNA的特异性抗体。结果：成功体外合成具有稳定性的JSRV-env mRNA；重组了基于慢病毒质粒结构的JSRV假病毒,且具有感染能力；假病毒体外中和试验检测出JSRV-env mRNA免疫小鼠体内产生了针对JSRV的特异性抗体。结论：针对JSRV病毒包膜的信使RNA，经皮下注射到小鼠体内，能够表达产生针对JSRV-env的中和抗体。
Abstract:
Objective: To detect the specific antibodies of JSRV-env mRNA immunized mice by pseudovirus neutralization method.Methods: The PT7TS vector was optimized before inserted in the target sequence. The recombinant plasmid of PT7TS-JSRV-env was verified by electrophoretic. After verifying its correctin in vitro， the JSRV-env mRNA wassynthesized. The BALB / c mice were given subcutaneous injection with protamine and JSRV-env mRNA complex. The specific antibodies of JSRV-env mRNA immunized mice was determined by the pseudovirus neutralizing antibody test. The JSRV pseudovirus was produced by the lentivirus packaging system. Results: The JSRV-env mRNA was successfully synthesized in vitro. The JSRV pseudovirus was recombined and presented powerful infection ability. The neutralization test of the sample serum in immunized mice and JSRV pseudovirus verified the JSRV pseudovirus.Conclusion:JSRV-envproducing antibodies can be expressed in mice by subcutaneous injections into JSRV-enveloped messenger RNA.

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相似文献/References:

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备注/Memo

备注/Memo:
基金项目 ?国家自然科学基金资助项目(81672650)

作者简介 白洁（1990-），女，硕士在读，研究方向：转录机制及疾病的生物治疗；

通信作者：朱泽，E-mail:zhuze@tijmu.edu.cn。

更新日期/Last Update: 2017-05-17