[1]任梦萌,王伟占,方 涛,等.替米沙坦对胰岛素抵抗大鼠PPAR-?蛋白表达的影响[J].天津医科大学学报,2017,23(02):124-127.
　REN Meng-meng,WANG Wei-zhan,FANG Tao,et al.Effects of telmisartan on the expression of PPAR-? protein in OLETF rats with insulin resistance[J].Journal of Tianjin Medical University,2017,23(02):124-127.

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替米沙坦对胰岛素抵抗大鼠PPAR-?蛋白表达的影响(PDF)

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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

23卷

期数:

2017年02期

页码:

124-127

栏目:

基础医学

出版日期:

2017-03-20

文章信息/Info

Title:

Effects of telmisartan on the expression of PPAR-? protein in OLETF rats with insulin resistance

文章编号:

1006-8147(2017)02-0124-04

作者:

任梦萌¹; 2; 王伟占²; 方 涛³; 田凤石²

（1.天津医科大学研究生院，天津 300070；2.天津市第四中心医院心内科，天津 300140；3. 天津市第四中心医院中心实验室，天津 300140）

Author(s):

REN Meng-meng¹; 2; WANG Wei-zhan²;  FANG Tao³;  TIAN Feng-shi²

(1.Graduate School, Tianjin Medical University, Tianjin 300070, China; 2. Department of Cardiology, The Fourth Center Hospital of Tianjin, Tianjin 300140, China; 3. Center Laboratory, The Fourth Center Hospital of Tianjin, Tianjin 300140, China)

关键词:

胰岛素抵抗; 替米沙坦; 吡格列酮; 过氧化物酶体增殖物激活受体?;  大鼠

Keywords:

insulin resistance;  telmisartan;  pioglitazone;  peroxisome; rat proliferators-activated receptor ? ;  yat

分类号:

R58

DOI:

-

文献标志码:

A

摘要:

目的： 观察替米沙坦对胰岛素抵抗（IR）大鼠肝脏、肾周脂肪和骨骼肌组织PPAR?蛋白表达的影响。方法： 雄性OLETF大鼠40只，高脂饲养20周建立IR模型后，随机分为4组（n=10只），即高脂对照组(O）、吡格列酮组(P）、低剂量替米沙坦组(VT）和替米沙坦组(T），进行药物干预，O组单纯高脂喂养，P组给予高脂加吡格列酮，VT组给予高脂加低剂量替米沙坦，T组给予高脂加替米沙坦。LETO大鼠12只为NC（正常对照）组，单纯予标准饲料。干预20周后测定各组大鼠FBG、FINS、HOMA-IR、ISI、TC、TG、HDL-C、LDL-C水平。Western blot测定各组织PPAR-?蛋白表达。结果： 40只OLETF大鼠均出现IR，造模成功。干预20周后，O组 FBG、 FINS、 HOMAIR、 TC、 TG、和LDL-C水平均高于P、VT和T组， ISI、 HDL-C均低于P、VT和T组，根据Western结果可看出P、VT、T组所取各组织PPAR-?蛋白表达均高于O组（均P＜0.05）；VT组 FBG、 FINS、 HOMAIR、 TC、 TG、和LDL-C水平均高于T组，ISI、 HDL-C水平低于T组，且T组比VT组各组织PPAR-?蛋白表达较高（均 P＜0.05）。结论： 替米沙坦可通过上调肝脏、肾周脂肪及骨骼肌组织PPAR-?蛋白的表达来改善IR，且改善程度可能与替米沙坦剂量有关。

Abstract:

Objective: To observe the effects of telmisartan on the expression of PPAR-? protein in rats tissues including liver, muscle and perirenal fat with IR. Methods: Forty male OLETF rats were fed with high-fat diet for 20 weeks to establish the IR model, then randomly assigned into four groups: the high-fat diet set(O) were given pure high-fat diet, the Pioglitazone group (P) were fed with high-fat diet and Pioglitazone, the low dose Telmisartan group (VT) were fed with high-fat diet and low dose Telmisartan, and rats in the Telmisartan group (T) were fed with high-fat diet and Telmisartan. Twelve LETO rats raised by standard feeding served as normal control group (NC). Following 6 weeks-drug intervention, the plasma levels of FBG, FINS, HOMA-IR, ISI, TC, TG, HDL-C, LDL-C were detected in four groups, and the expression level of PPAR-? protein was measured using Western blot in each tissues . Results: Insulin resistance was observed in all 40 rats and buildings were established. Compared with group P, VT and T, the FBG, FINS, HOMA-IR, TC, TG, and LDL-C in O group were significantly increased. ISI and HDL-C were decreased (P <0.05). The expressions of PPAR? protein in group P, VT and T were higher than group O. And the group T was lower than VT group in biochemical index results except for ISI and HDL-C; and the expression of PPAR? protein was higher. Conclusion: Telmisartan can significantly improve IR through increasing the expression of PPAR-? protein in liver, perirenal fat and skeletal muscle tissues; and the improvement degree may be associated with telmisartan dose.

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备注/Memo

备注/Memo:

基金项目 天津市应用基础与前沿技术研究计划（青年项目）基金资助（15JCQNJC13100）
作者简介 任梦萌（1990-），女，硕士在读，研究方向：心血管内科；

通信作者：田凤石，Email：fengshitian0801@hotmail.com。

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更新日期/Last Update: 2017-03-28