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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

23卷

期数:

2017年01期

页码:

1-04

栏目:

基础医学

出版日期:

2017-01-16

文章信息/Info

Title:

Conditional medium from electric pulse-stimulated C2C12 skeletal muscle cells reverses insulin resistance and endothelial dysfunction

in endothelial cells

文章编号:

1006-8147（2017）01-0001-04

作者:

赵一贺; 张 畅; 牛文彦

（天津医科大学免疫学系，天津300070）

Author(s):

ZHAO Yi-he;  ZHANG Chang;  NIU Wen-yan

(Department of Immunology, Tianjin Medical University, Tianjin 300070, China)

关键词:

内皮功能障碍; 胰岛素抵抗; 炎症; 糖尿病

Keywords:

endothelial dysfunction;  insulin resistance;  inflammation;  diabetes

分类号:

R392.1

DOI:

-

文献标志码:

A

摘要:

目的：探讨电脉冲刺激的C2C12肌管细胞条件培养基对内皮细胞的影响。方法：提取常氧和缺氧培养的3T3-L1脂肪细胞上清液为条件培养基CM-N和CM-H，电脉冲刺激的C2C12肌管细胞上清液为条件培养基CM-EPS，单独或按比例混合分别孵育内皮细胞16h。Western blot 检测Akt、eNOS、IKK和NF-кB的磷酸化。结果：CM-H降低内皮细胞Akt和eNOS的磷酸化，并升高IKK和NF-кB的磷酸化。CM-EPS逆转此作用。结论：CM-EPS逆转CM-H造成的内皮细胞胰岛素抵抗并改善内皮功能。

Abstract:

Objective: To explore the effects of conditional medium from electric pulse-stimulated C2C12 myotube on endothelial insulin resistance and dysfunction. Methods: The supernatant of normoxic and hypoxic 3T3-L1 adipocytes were collected as conditional medium CM-N and CM-H, respectively. The supernatant of pulsed electrical-stimulated C2C12 myotube was collected as conditional medium CM-EPS. Endothelial cells were incubated with CM-N, CM-H and CM-EPS for 16 hours, respectively. The phosphorylation of Akt, eNOS, IKK and NF-кB in endothelial cells were analyzed by Western blot. Results: The phosphorylation of Akt and eNOS were increased significantly, and the phosphorylation of IKK and NF-кB were reduced under CM-H treatment, which were reversed by adding CM-EPS. Conclusion: CM-EPS can reverse insulin resistance in endothelial cells and improve endothelial dysfunction induced by CM-H.

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备注/Memo

备注/Memo:

基金项目 国家自然科学基金资助项目（81170740,81161120545）
作者简介 赵一贺（1990-），男，硕士在读，研究方向：免疫学；通信作者：牛文彦，E-mail: wniu@tmu.edu.cn。

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更新日期/Last Update: 2017-01-15