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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

22卷

期数:

2016年06期

页码:

478-482

栏目:

基础医学

出版日期:

2016-11-20

文章信息/Info

Title:

IL-11 enhances expression of PMP22 in cultured Schwann cells via gp130/JAK2/STAT3 signaling pathway

文章编号:

1006-8147（2016）06-0478-5

作者:

郭 巍; 李 岩; 段会全; 孙 超; 冯世庆; 徐云强

（天津医科大学总医院骨科，天津 300052）

Author(s):

GUO Wei;  LI Yan;  DUAN Hui-quan;  SUN Chao;  FENG Shi-qing;  XU Yun-qiang

（Department of Orthopaedics , General Hospital, Tianjin Medical University，Tianjin 300052， China）

关键词:

白介素-11; 雪旺细胞; 周围神经髓鞘蛋白22; JAK2/STAT3信号通路; 大鼠

Keywords:

interleukin-11; Schwann cells; PMP22; JAK2/STAT3 signaling pathway; rat

分类号:

R68

DOI:

-

文献标志码:

A

摘要:

目的：探索白介素-11对大鼠雪旺细胞分泌髓鞘蛋白的调节作用及相关机制。方法：取成年Wistar大鼠坐骨神经，分离纯化培养雪旺细胞。加入重组大鼠白介素-11，分别采用realtime RT-qPCR和Western boltting检测雪旺细胞髓鞘蛋白的表达情况，进一步检测IL-11调控髓鞘蛋白表达的相关通路的激活情况。结果：Realtime RT-qPCR和Western boltting结果均提示白介素-11可以显著提高雪旺细胞中周围神经髓鞘蛋白22的表达水平，而对雪旺细胞中髓鞘碱性蛋白和髓鞘蛋白零的表达水平则没无明显影响，该生理作用可被JAK特异性抑制剂AG490所抑制。在经过IL-11处理的SCs 细胞中可以观察到pJAK2（Y1007＋Y1008）和pSTAT3（Y705）含量明显高于对照组且伴随着SCs中gp130表达的上调。结论：白介素-11可通过调剂gp30/JAK2/STAT3信号通路以促进雪旺细胞中周围神经髓鞘蛋白22的表达。

Abstract:

Objective ：To examine the effect of IL-11 on the expression of myelin proteins in cultured Schwann cells. Methods： Schwann cells were cultured and purified from sciatic nerves of adult Wistar rats. IL-11 was used to treat purified Schwann cells. The expression level of myelin proteins was examined with real time RT-qPCR and western boltting. Results： IL-11 significantly increased the expression level of PMP22 in Schwann cells, but not those of MPZ and MBP. And this effect was significantly inhibited by AG490, anspecificinhibitor of JAK. And the levels of JAk2 and STAT3 phosphorylation were significantly increased in the presence of IL-11 stimulatedSchwann cells compared with the absence of IL-11, which was accompanied with the up-regulation of gp130. Conclusion： IL-11 can enhance the production of PMP22 in Schwann cells via gp130/JAK2/STAT3 signaling pathway and thus may contribute to myelination.

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相似文献/References:

[1]潘大宇,宁广智,冯世庆.雪旺细胞外泌体影响胶质瘢痕形成修复小鼠脊髓损伤的实验研究[J].天津医科大学学报,2020,26(05):397.
　PAN Da-yu,NING Guang-zhi,FENG Shi-qing.Schwann cell-derived exosomes promote functional recovery of spinal cord injury in mice via affecting glial scar formation[J].Journal of Tianjin Medical University,2020,26(06):397.

备注/Memo

备注/Memo:

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作者简介? 郭巍（1989-），男，硕士在读，研究方向：髓鞘再生；通信作者：徐云强，E-mail: docxu@sina.com。

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更新日期/Last Update: 2016-11-25