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[1]陈 爽,赵亚倩,苟 芸,等.大鼠脑缺血再灌注后线粒体损伤及p-STAT3表达[J].天津医科大学学报,2016,22(06):469-473.
　CHEN Shuang,ZHAO Ya-qian,GOU Yun,et al.Mitochondrial damage and p-STAT3 protein expression following focal cerebral ischemia-reperfusion in rats[J].Journal of Tianjin Medical University,2016,22(06):469-473.
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
22卷
期数:
2016年06期
页码:
469-473
栏目:
基础医学
出版日期:
2016-11-20

文章信息/Info

Title:
Mitochondrial damage and p-STAT3 protein expression following focal cerebral ischemia-reperfusion in rats
文章编号:
1006-8147（2016）06-0469-5
作者:
陈 爽; 赵亚倩; 苟 芸; 黄国伟; 张绪梅
(天津医科大学公共卫生学院营养与食品卫生学系,天津300070)
Author(s):

CHEN Shuang;  ZHAO Ya-qian;  GOU Yun;  HUANG Guo-wei; ZHANG Xu-mei

?(Department of Nutrition and Food Hygiene, School of Public Health, Tianjin Medical University, Tianjin 300070, China)
关键词:
缺血再灌注; 脑组织线粒体; 信号转导和转录激活子3; 大鼠
Keywords:
ischemia-reperfusion injury;  cerebral mitochondria;  signal transducer and activator of transcription 3; rat
分类号:
R743.3
DOI:
-
文献标志码:
A
摘要:
目的：研究大鼠脑缺血再灌注后脑组织线粒体中信号转导与激活子3（STAT3）的表达情况，探讨线粒体中磷酸化STAT3（p-STAT3）与脑缺血后线粒体损伤的关系。方法：线栓法制作大鼠脑局部缺血再灌注模型，大鼠随机分为假手术（SHAM）组和大脑中动脉栓塞再灌注（I/R）组。透射电镜观察皮质区神经元内的线粒体损伤情况。Western blotting及免疫荧光双标法检测线粒体中STAT3及p-STAT3表达。结果：与SHAM组比较，大鼠脑缺血再灌注后，皮质区神经元内的线粒体损伤严重；线粒体中存在STAT3表达，且在脑缺血再灌注后表达量无变化,差异没有统计学意义（P>0.05），而p-STAT3表达显著增加，差异具有统计学意义（P<0.05）。结论：STAT3在大鼠脑组织线粒体中表达，脑缺血再灌注后被显著激活，推测脑缺血再灌损伤后，线粒体中STAT3的激活可能参与了脑缺血再灌注病理生理过程中线粒体的损伤和修复。
Abstract:
Objective: To assess the expression and activation of signal transducer and activator of transcription 3 (STAT3) in mitochondria of rat brain cortex, and toexplore the role of phosphorylated STAT3 (p-STAT3) in mitochondrial injury and repair following cerebral ischemia and reperfusion. Methods: The model of focal cerebral ischemia-reperfusion was established by suture method. The rats were randomly divided into the sham-operated (SHAM) group and the middle cerebral occlusion-reperfusion (I/R) group. The ultrastructure of cortex mitochondria was observed under transmission electronic microscope. The expressions of total STAT3 and p-STAT3 were detected by western blotting and immunofluorescent double-staining. Results: A significant mitochondrial ultrastructural injury was found in I/R group, compared with the SHAM group STAT3. Immunoreactivity was observed in the mitochondria of rat brain cortex. No significantdifference in total STAT3 level was detected in both experimental groups (P>0.05), whereas the level of p-STAT3 in the mitochondria of brain cortex was elevated after ischemia injury (P<0.05). Conclusion: STAT3 localizes in mitochondria. The activity of mitochondrial STAT3 is elevated during cerebral ischemia-reperfusion. STAT3 activation may be involved in mitochondrial injury and repair in the pathophysiological process of cerebral ischemia-reperfusion injury.

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备注/Memo

备注/Memo:

基金项目 国家自然科学基金资助项目（81373003）；中国博士后科学基金资助项目（2014M 550148）

作者简介 陈爽（1990-），女，硕士在读，研究方向：营养与神经退行性疾病；通信作者：张绪梅，E-mail：zhangxumei@tijmu.edu.cn。

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更新日期/Last Update: 2016-11-25