|本期目录/Table of Contents|

[1]王丽宁,范晓东,孙二琳,等.重组hIFN-α-2b-BCG抗肿瘤效应的体外研究[J].天津医科大学学报,2016,22(05):377-380.
　WANG Li-ning,FAN Xiao-dong,SUN Er-lin,et al.Study on antitumor effect of recombinant hIFN-α-2b-BCG in vitro[J].Journal of Tianjin Medical University,2016,22(05):377-380.
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重组hIFN-α-2b-BCG抗肿瘤效应的体外研究(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
22卷
期数:
2016年05期
页码:
377-380
栏目:
基础医学
出版日期:
2016-09-20

文章信息/Info

Title:
Study on antitumor effect of recombinant hIFN-α-2b-BCG in vitro
文章编号:
1006-8147（2016）05-0377-04
作者:
王丽宁; 范晓东; 孙二琳; 李 娜; 卢炳新; 雷铭德; 丁 娜; 孙 岩; 韩瑞发
(天津医科大学第二医院泌尿外科,天津市泌尿外科研究所,天津 300211)
Author(s):
?WANG Li-ning;  FAN Xiao-dong;  SUN Er-lin;  LI Na;  LU Bing-xin;  LEI Ming-de;  DING Na;  SUN Yan;  HAN Rui-fa

（Department of Urology，The Second Hospital ， Tianjin Medical University，Tianjin Institute of Urology， Tianjin 300211，China）

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关键词:
重组BCG; IFN-α-2b; 膀胱肿瘤; 免疫疗法
Keywords:
?recombinant BCG' target="_blank" rel="external">">?recombinant BCG;  IFN-α-2b;  bladder tumor; immunotherapy
分类号:
R737.14
DOI:
-
文献标志码:
A
摘要:
目的：了解重组hIFN-α-2b-BCG (rBCG) 体外抗肿瘤的作用效果和作用机制。方法：体外共培养后，透射电镜观察不同时间点rBCG对人膀胱肿瘤EJ细胞的影响。吖啶橙染色观察各组肿瘤细胞形态。MTT法检测rBCG对肿瘤细胞生长抑制率。ELISA检测rBCG作用后淋巴细胞分泌Th1型细胞因子水平。LDH释放试验检测rBCG激活的淋巴细胞对肿瘤细胞杀伤效应。结果：BCG和rBCG作用后的肿瘤细胞在透射电镜下和吖啶橙染色后均有显著变化。MTT显示rBCG的生长抑制率显著高于BCG和BCG+IFN组。rBCG对淋巴细胞分泌Th1型细胞因子有影响，同时rBCG激活的淋巴细胞对膀胱肿瘤细胞的杀伤作用。结论：重组BCG在体外有优于BCG的免疫调节特性、抗肿瘤作用和直接细胞毒作用。
Abstract:

Objective: To study antitumor effect and mechanism of action on recombinant IFN-α-2b-BCG in vitro. Methods: Effect of rBCG on bladder tumor cells was observed through transmission electro microscopy and microscope. Acridine orange staining was used to observe the tumor cellsmorphology by fluorescence microscope. Growth? inhibition rate on tumor cell was detected by MTT assay. Effect of rBCG cultures on secretion of Th1-type cytokines was detected by ELISA assay. LDH release assay was applied to determine the lethal effect of active lymphocyte which was stimulated by BCG on umor cells. Results:The rBCG group showed significant changes. MTT showed rBCG inhibited the proliferation of bladder cancer cell, and more active other groups. RBCG cultures affected the section of Th1 cytokines. And rBCG could enhanced the lethal effect of lymphocyte on tumor cells. Conclusion: The rBCG may have more immunomodulatory properties, anti-tumor effects and cytotoxicity in vitro.

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备注/Memo

备注/Memo:

基金项目 国家自然科学基金资助项目（81402095）；天津市科委抗癌重大科技专项基金资助项目(12zcdzsy16900)；天津市卫计委科技基金资助项目（2014KZ104）

作者简介 王丽宁（1989-），女，研究实习员，学士，研究方向：泌尿外科基础医学；

通信作者：孙二琳，E-mail：irene_sunsun@163.com。

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更新日期/Last Update: 2016-09-19