|本期目录/Table of Contents|

[1]张文晓,张碧丽.阿霉素肾病大鼠血清IL-17、IL-6的动态变化及其意义[J].天津医科大学学报,2016,22(03):204-207.
　ZHANG Wen-xiao,ZHANG Bi-li.Dynamic changes and significance of serum IL-17 and IL-6 in adriamycin-induced nephropathy rats[J].Journal of Tianjin Medical University,2016,22(03):204-207.
点击复制

阿霉素肾病大鼠血清IL-17、IL-6的动态变化及其意义(PDF)
分享到：

《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
22卷
期数:
2016年03期
页码:
204-207
栏目:
基础医学
出版日期:
2016-05-20

文章信息/Info

Title:
Dynamic changes and significance of serum IL-17 and IL-6 in adriamycin-induced nephropathy rats
文章编号:
1006-8147（2016）03-0204-04
作者:
?张文晓 ; 张碧丽
?（天津市儿童医院肾脏科，天津 300134）
Author(s):
?ZHANG Wen-xiao; ZHANG Bi-li
?（Department of Nephrology，Tianjin Children’s Hospital，Tianjin 300134，China）
关键词:
阿霉素肾病; 肾病综合征; Th17细胞; IL-17; IL-6 ; 大鼠
Keywords:
adriamycin-induced nephrosis; nephrotic syndrome; Th17 cells; IL-17; IL-6;  rat
分类号:
R692
DOI:
-
文献标志码:
A
摘要:
?目的： 探究阿霉素肾病大鼠血清白细胞介素（IL）-17、IL-6的动态变化意义及泼尼松通过2种因子对阿霉素肾病的干预机制。方法： 将81只大鼠随机分为空白对照组、模型组、泼尼松干预组，每组27只，通过分次尾静脉注射法建立阿霉素肾病大鼠模型，检测4、6、8周末大鼠血生化、24 h尿蛋白定量及尿肌酐变化，计算内生肌酐清除率；应用 ELISA检测大鼠血清IL-17、IL-6水平的变化。结果： 实验第4周末，与空白对照组比较，模型组和泼尼松干预组大鼠出现大量蛋白尿、低白蛋白血症和高脂血症（P<0.05），提示模型建立成功；模型组和泼尼松干预组大鼠血清IL-17、IL-6水平高于空白对照组。实验第6、8周末，与模型组比较，泼尼松干预组24 h尿蛋白定量、血肌酐、总胆固醇显著降低 （P<0.05），血清总蛋白、白蛋白、内生肌酐清除率显著升高（P<0.05）；泼尼松干预组大鼠血清IL-17、IL-6水平明显低于模型组（P<0.05）。结论： IL-17、IL-6参与了阿霉素肾病的免疫发病机制，泼尼松可能通过降低血清IL-17、IL-6水平参与阿霉素肾病的治疗过程。
Abstract:
Objective ： To explore the significance of dynamic change of serum interleukin (IL) -17,IL-6 in adriamycin(ADR)-induced nephropathy rats and the mechanism of prednisone intervention in ADR-induced nephropathy. Methods： Eighty-one rats were randomly divided into control group, model group, and prednisone intervention group. ADR model was induced by a tail intravenous injection of ADR twice. The blood biochemical indexes and 24 h urinary protein, urine creatinine were measured at the end of 4, 6 and 8 weeks. The values of creatinine clearance (Ccr) were calculated by the formula. The expressions of serum IL-17 and IL-6 were detected by ELISA. Results：, heavy proteinuria, hypoalbuminemia, hyperlipemia were measured in other groups at the end of 4 weeks compared with control group ,indicating that the models were successfully established. The levels of IL-17 and IL-6 in model group and prednisone intervention group were higher than control group at the end of 4 weeks(P < 0.05). At the end of 6 and 8 weeks, compared with model group, 24 h proteinuria, urine creatinine,total cholesterol, serum creatinine, blood urea nitrogen in prednisone intervention group were decreased significantly (P < 0.05), while serum total protein, albumin, endogenous creatinine clearance rate were increased significantly (P < 0.05). The expressions of serum IL-17 and IL-6 in prednisone intervention group werelower than control group(P < 0.05). Conclusion ： IL-17 and IL-6 may be involved in the immune pathogenesis of ADR-induced nephropathy rats. Prednisone could treat ADR-induced nephropathy by reducing the serum levels of IL-17 and IL-6.

参考文献/References:

[1] Pan Q, Wu J, Tao J, et al.Role of basophils in the pathogenesis of minimal change nephrotic syndrome: A literature review[J]. Exp Ther Med. 2014，8(4): 1027

[2] Prasada N ，Jaiswala A K，Agarwalb V ，et al．Differential alteration in peripheral T-regulatory and T-effector cells with change in P-glycoprotein expression in childhood nephrotic syndrome: A longitudinal study[J]．Cytokine，2015，72(2)：190

[3] Harrington L E, Hatton R D, Mangan P R，et al．Interleukin 17-producing CD4 + effector T cells develop via a lineage distinct from the T helper type 1 and 2 lineages[J]．Nat Immunol，2005，6 ( 11 ):1123

[4] Lin P. Targeting interleukin-6 for noninfectious uveitis[J]. Clin Ophthalmol , 2015,11(9):1697

[5] Youn Y S, Lim H H, Lee J H. The clinical characteristics of steroid responsive nephrotic syndrome of children according to the serum immunoglobulin E levels and cytokines[J]. Yonsei Med J, 2012, 53(4): 715

[6] Valentin-Acevedo A, Covey L R, Gurkan S. B lymphocyte cytokine profile in a monozygotic twin pair discordant for nephrotic syndrome[J]. Indian J Pediatr, 2013, 80(10): 875

[7] Hünem?rder S, Treder J, Ahrens S，et al. Th1 and Th17 cells promote crescent formation in experimental autoimmune glomerulonephritis[J]. J Pathol , 2015,237(1):62

[8] Wei P, Hu G H, Kang H Y, et al. An aryl hydrocarbon receptor ligand acts on dendritic cells and T cells to suppress the Th17 response in allergic rhinitis patients[J]. Lab Invest, 2014，94(5):528

[9] Hreggvidsdottir H S, Noordenbos T, Baeten D L.．Inflammatory pathways in spondyloarthritis[J]．Mol Immunol，2014，57 (1):28

[10] Zheng L, Ye P, Liu C. The role of the IL-23/IL-17 axis inthe pathogenesis of Graves’ disease[J]. Endocr J, 2013,60(5):591

[11]盖丰丰,曹鹏,鲍浩,等. Th17细胞及相关细胞因子与肾脏疾病[J]. 肾脏病与透析肾移植杂志, 2012,21(2):170

[12] Wang L，Li Q，Wang L，et al．The role of Th17/IL-17 in the pathogenesis of primary nephrotic syndrome in children[J]．Kidney Blood Press Res，2013，37(4/5):332

[13] Savin V J, Sharma M, Zhou J, et al. Renal and hematological effects of CLCF-1, a B-cell-stimulating cytokine of the IL-6 family[J]. J Immunol Res, 2015, 2015:714

[14] Wang L M，Chi Y J，Wang L N，et al．Expression of interleukin-6 in rat model of doxorubicin-induced nephropathy[J]．CJCP，2010，12(11): 912

[15]杨维娜,于琳华,郭尚温,等. 改良阿霉素肾病大鼠模型的建立[J]. 西安交通大学学报(医学版),2009,30(4):445

[16] 王丽敏,邓李玲,郑婵娟,等. 白介素-18结合蛋白对阿霉素肾病大鼠Fractalkine表达的影响[J]. 临床儿科杂志, 2012,30(7):666

相似文献/References:

[1]刘 艳,张碧丽,王文红,等.儿童肾病综合征合并真菌感染临床分析[J].天津医科大学学报,2013,19(06):496.

备注/Memo

备注/Memo:
基金项目 天津市应用基础与前沿技术研究计划项目（13JCYBJC22300）

作者简介 张文晓（1990-），女，硕士在读，研究方向：儿科学；

通信作者:张碧丽，E-mail：zhangbili218@163.com。

更新日期/Last Update: 2016-05-24