|本期目录/Table of Contents|

[1]邹 磊,王玉华,蔡春友,等.PCAF通过调控FOXO1活性参与3T3-L1前脂肪细胞分化[J].天津医科大学学报,2015,21(03):203-207.
　ZOU Lei,WANG Yu-hua,CAI Chun-you,et al.P300/CBP associated factor regulates adipogenesis of 3T3-L1 cells by controlling FOXO1[J].Journal of Tianjin Medical University,2015,21(03):203-207.
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PCAF通过调控FOXO1活性参与3T3-L1前脂肪细胞分化(PDF)
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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:
21卷
期数:
2015年03期
页码:
203-207
栏目:
基础医学
出版日期:
2015-05-20

文章信息/Info

Title:
P300/CBP associated factor  regulates adipogenesis of 3T3-L1 cells by controlling FOXO1
文章编号:
1006-8147（2015）03-0203-05
作者:
邹 磊; 王玉华; 蔡春友; 魏凤江; 杨付花; 焦红肖; 凌 超; 时文涛; 李卫东
(天津医科大学基础医学研究中心,天津300070)
Author(s):

 ZOU Lei;  WANG Yu-hua;  CAI Chun-you;  WEI Feng-jiang;  YANG Fu-hua;  JIAO Hong-xiao;  LING Chao;  SHI Wen-tao;  LI Wei-dong

 (Research Center of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China)
关键词:
PCAF; 3T3-L1细胞; FOXO1; 转录调控
Keywords:
P300/CBP associated factor ;  3T3-L1 cell line;  FOXO1;  transcriptional control
分类号:
Q254
DOI:
-
文献标志码:
A
摘要:
目的：在3T3-L1细胞模型中，探讨组蛋白乙酰转移酶PCAF对脂肪细胞分化调控的分子机制。方法：应用Real-time PCR和Western blot检测PCAF在3T3-L1细胞分化过程中的表达；应用重组慢病毒感染细胞，shRNA干扰或过表达PCAF，通过Oil-Red-O染色法观察PCAF表达量变化对细胞分化的影响；应用ChIP on chip和PCR array的方法筛选PCAF调控的下游基因和通路；应用Western blot和ChIP qPCR对潜在目的基因进行验证。结果：PCAF在3T3-L1细胞分化过程中表达升高；PCAF表达下调会抑制脂肪细胞的分化；PCAF可以在细胞分化不同时间点诱导PDPK1和FOXO1的表达，并间接影响了FOXO1磷酸化。结论：组蛋白乙酰转移酶PCAF通过胰岛素通路（PI3K/PDPK1/AKT/FOXO1）参与对脂肪细胞分化的转录调控。
Abstract:
Objective: To investigate the molecular mechanism of PCAF gene in regulation of adipocytes differentiation. Methods: PCAF expression levels were measured by Real-time PCR and Western blot in 3T3-L1 cells during differentiation; 3T3-L1 cells were transfected by recombinant Lentiviral ORF/shRNA and the effects of PCAF overexpression or knockdown on differentiation were observed by Oil-Red-O staining; ChIP on chip and PCR array were used to screen the signaling pathways regulated by PCAF; Expressions of potential target genes and pathways were detected by Western blot and ChIP qPCR. Results: PCAF expression increased during 3T3-L1 cells differentiation and adipogenesis; the differentiation of 3T3-L1 might be interfered by knockdown of PCAF ; Expression of PDPK1 and FOXO1 were induced by PCAF at various time points during 3T3-L1 differentiation; the phosphorylation of FOXO1 was also regulated by PCAF indirectly. Conclusion: PCAF regulates preadipocytes differentiation through the insulin pathway (PI3K/PDPK1/AKT /FOXO1).

参考文献/References:

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备注/Memo

备注/Memo:
基金项目 国家自然科学基金资助项目（31201029,81070576）；教育部博士点基金资助项目（20111202120002）；天津市应用基础及前沿技术研究计划（12JCZDJC24700）
作者简介 邹磊（1984-），男，硕士在读，研究方向：病理与病理生理学；

通信作者：时文涛，shiwentao@tijmu.edu.cn；李卫东，liweidong98@tijmu.edu.cn。

更新日期/Last Update: 2015-05-27