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《天津医科大学学报》[ISSN:1006-8147/CN:12-1259/R]

卷:

20卷

期数:

2014年05期

页码:

350-353

栏目:

基础医学

出版日期:

2014-09-20

文章信息/Info

Title:

Dual antiplatelet drugs-induced small intestinal injury and its possible mechanism in rats

文章编号:

1006-8147(2014)05-0350-04

作者:

田珺琪¹; 张志广¹; 李 熳¹; 刘 霞¹; 张雪莲¹; 陆 伟²

(1.天津医科大学第二医院消化科,天津300211；2.天津市第二人民医院消化科,天津300192)

Author(s):

TIAN Jun-qi¹;  ZHANG Zhi-guang¹;  LI Man¹;  LIU Xia¹;  ZHANG Xue-lian¹; LU Wei²

(1.Department of Gastroenterology, The Second Hospital, Tianjin Medical University, Tianjin 300211, China;2.Department of Gastroenterology, Tianjin Second  Peoples^,s Hospital Tianjin 300192, China)

关键词:

抗血小板药物; 阿司匹林; 氯吡格雷; 小肠损伤; TNF-α; IL-1β; 大鼠

Keywords:

antiplatelet drugs; aspirin; clopidogrel; small intestinal injury; TNF-α; IL-1β; rat

分类号:

R574.5

DOI:

-

文献标志码:

A

摘要:

目的:探讨双联抗血小板药物对大鼠小肠的损伤及其机制。方法：SD大鼠80只随机分为4组，每组20只：阴性对照组、阿司匹林组、氯吡格雷组、阿司匹林联合氯吡格雷组（双抗组），分别予生理盐水、阿司匹林(10.41 mg/kg)、氯吡格雷(7.81 mg/kg)、阿司匹林(10.41 mg/kg)联合氯吡格雷(7.81 mg/kg)灌胃1次/d，共14 d。所有大鼠于末次给药后手术，观察小肠的损伤情况；免疫组化法测定小肠黏膜细胞肿瘤坏死因子-α( TNF-α）及白细胞介素-1β( IL-1β)的表达水平。结果：（1）各实验组大鼠小肠的损伤程度均高于对照组（P<0.01），且阿司匹林组高于氯吡格雷组（P<0.01），双抗组最高（P<0.01)；（2）各实验组大鼠小肠黏膜TNF-α、IL-1β较对照组均呈明显高水平表达（P<0.05)，且阿司匹林组高于氯吡格雷组（P<0.05)，双抗组最高（P<0.05)。结论：常规剂量抗血小板药物可造成大鼠小肠损伤，且联合用药较单一用药损伤程度重，同时其TNF-α、IL-1β表达增强，提示这种变化可能参与了小肠损伤。

Abstract:

Objective：To investigate dual antiplatelet drugs-induced small intestinal injury and its possible mechanism in rats. Methods： Eighty SD rats were randomly divided into four groups, with n=20 in each group: negative control group, aspirin group, clopidogrel group and clopidogrel combined aspirin group. Four groups were given normal saline，aspirin (10.41 mg/kg)，clopidogrel (7.81 mg/kg) and clopidogrel(7.81 mg/kg) combined aspirin(10.41 mg/kg) respectivelyas intragastric administration once a day for 14 days. All rats received operation after the final intragastric administration, then small intestinal lesions were observed , and the expression level of tumor necrosis factor-α（TNF-α）and interleukin-1β（IL-1β）in small intestinal mucosal cells was detected by immunohistochemistry method. Results：（1）Small intestinal lesions in rats of experiment groups were more serious than the control group（P<0.01）, the aspirin group was more serious than the clopidogrel group（P<0.01）, and the double antiplatelet group had the most serious damage(P<0.01);（2）The expression levels of TNF-α and IL-1βon small intestinal mucosa in all experiment group rats were higher than those in the control group（P<0.05), the aspirin group was higher than the clopidogrel group（P<0.05）, and the double antiplatelet group had the highest levels（P<0.05）.Conclusion： Routine dose of antiplatelet drugs can cause small intestinal injury in rats, and drug combination aggravates the small intestinal injury compared with that of single drug. Furthermore, the expression levels of TNF-α and IL-1β were higher, which may lead to small intestinal mucosal injury.

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相似文献/References:

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　TIAN Jun-qi,ZHANG Zhi-guang,LI Man,et al.Dual antiplatelet drugs-induced small intestinal injury and its possible mechanism in rats[J].Journal of Tianjin Medical University,2014,20(05):0.
[3]田珺琪,张志广,李 熳,等.双联抗血小板药物对大鼠小肠的损伤及其机制[J].天津医科大学学报,2014,20(05):0.
　TIAN Jun-qi,ZHANG Zhi-guang,LI Man,et al.Dual antiplatelet drugs-induced small intestinal injury and its possible mechanism in rats[J].Journal of Tianjin Medical University,2014,20(05):0.
[4]田珺琪,张志广,李 熳,等.双联抗血小板药物对大鼠小肠的损伤及其机制[J].天津医科大学学报,2014,20(05):350.
　TIAN Jun-qi,ZHANG Zhi-guang,LI Man,et al.Dual antiplatelet drugs-induced small intestinal injury and its possible mechanism in rats[J].Journal of Tianjin Medical University,2014,20(05):350.

备注/Memo

备注/Memo:

基金项目 天津市卫生局科技基金资助项目（07KG8）
作者简介 田珺琪（1988-），女，硕士在读，研究方向：消化科；

通信作者：张志广，E-mail：zhiguang_zhang@sina.com。

常用功能

更新日期/Last Update: 2014-09-25